Cargando…
Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis
Cellular microvesicles and nanovesicles (exosomes) are involved in many disease processes and have major potential as biomarkers. However, developments in this area are constrained by limitations in the technology available for their measurement. Here we report on the use of fluorescence nanoparticl...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280380/ https://www.ncbi.nlm.nih.gov/pubmed/21601655 http://dx.doi.org/10.1016/j.nano.2011.04.003 |
_version_ | 1782223822023294976 |
---|---|
author | Dragovic, Rebecca A. Gardiner, Christopher Brooks, Alexandra S. Tannetta, Dionne S. Ferguson, David J.P. Hole, Patrick Carr, Bob Redman, Christopher W.G. Harris, Adrian L. Dobson, Peter J. Harrison, Paul Sargent, Ian L. |
author_facet | Dragovic, Rebecca A. Gardiner, Christopher Brooks, Alexandra S. Tannetta, Dionne S. Ferguson, David J.P. Hole, Patrick Carr, Bob Redman, Christopher W.G. Harris, Adrian L. Dobson, Peter J. Harrison, Paul Sargent, Ian L. |
author_sort | Dragovic, Rebecca A. |
collection | PubMed |
description | Cellular microvesicles and nanovesicles (exosomes) are involved in many disease processes and have major potential as biomarkers. However, developments in this area are constrained by limitations in the technology available for their measurement. Here we report on the use of fluorescence nanoparticle tracking analysis (NTA) to rapidly size and phenotype cellular vesicles. In this system vesicles are visualized by light scattering using a light microscope. A video is taken, and the NTA software tracks the brownian motion of individual vesicles and calculates their size and total concentration. Using human placental vesicles and plasma, we have demonstrated that NTA can measure cellular vesicles as small as ∼50 nm and is far more sensitive than conventional flow cytometry (lower limit ∼300 nm). By combining NTA with fluorescence measurement we have demonstrated that vesicles can be labeled with specific antibody-conjugated quantum dots, allowing their phenotype to be determined. FROM THE CLINICAL EDITOR: The authors of this study utilized fluorescence nanoparticle tracking analysis (NTA) to rapidly size and phenotype cellular vesicles, demonstrating that NTA is far more sensitive than conventional flow cytometry. |
format | Online Article Text |
id | pubmed-3280380 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-32803802012-02-24 Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis Dragovic, Rebecca A. Gardiner, Christopher Brooks, Alexandra S. Tannetta, Dionne S. Ferguson, David J.P. Hole, Patrick Carr, Bob Redman, Christopher W.G. Harris, Adrian L. Dobson, Peter J. Harrison, Paul Sargent, Ian L. Nanomedicine Research Article Cellular microvesicles and nanovesicles (exosomes) are involved in many disease processes and have major potential as biomarkers. However, developments in this area are constrained by limitations in the technology available for their measurement. Here we report on the use of fluorescence nanoparticle tracking analysis (NTA) to rapidly size and phenotype cellular vesicles. In this system vesicles are visualized by light scattering using a light microscope. A video is taken, and the NTA software tracks the brownian motion of individual vesicles and calculates their size and total concentration. Using human placental vesicles and plasma, we have demonstrated that NTA can measure cellular vesicles as small as ∼50 nm and is far more sensitive than conventional flow cytometry (lower limit ∼300 nm). By combining NTA with fluorescence measurement we have demonstrated that vesicles can be labeled with specific antibody-conjugated quantum dots, allowing their phenotype to be determined. FROM THE CLINICAL EDITOR: The authors of this study utilized fluorescence nanoparticle tracking analysis (NTA) to rapidly size and phenotype cellular vesicles, demonstrating that NTA is far more sensitive than conventional flow cytometry. Elsevier 2011-12 /pmc/articles/PMC3280380/ /pubmed/21601655 http://dx.doi.org/10.1016/j.nano.2011.04.003 Text en © 2011 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Research Article Dragovic, Rebecca A. Gardiner, Christopher Brooks, Alexandra S. Tannetta, Dionne S. Ferguson, David J.P. Hole, Patrick Carr, Bob Redman, Christopher W.G. Harris, Adrian L. Dobson, Peter J. Harrison, Paul Sargent, Ian L. Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis |
title | Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis |
title_full | Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis |
title_fullStr | Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis |
title_full_unstemmed | Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis |
title_short | Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis |
title_sort | sizing and phenotyping of cellular vesicles using nanoparticle tracking analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280380/ https://www.ncbi.nlm.nih.gov/pubmed/21601655 http://dx.doi.org/10.1016/j.nano.2011.04.003 |
work_keys_str_mv | AT dragovicrebeccaa sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis AT gardinerchristopher sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis AT brooksalexandras sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis AT tannettadionnes sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis AT fergusondavidjp sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis AT holepatrick sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis AT carrbob sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis AT redmanchristopherwg sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis AT harrisadrianl sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis AT dobsonpeterj sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis AT harrisonpaul sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis AT sargentianl sizingandphenotypingofcellularvesiclesusingnanoparticletrackinganalysis |