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Identification of potential apicoplast associated therapeutic targets in human and animal pathogen Toxoplasma gondii ME49

Toxoplasma gondii ME49 is an obligatory intracellular apicomplexa parasite that causes toxoplasmosis in humans, domesticated and wild animals. Waterborne outbreaks of acute toxoplasmosis worldwide reinforce the transmission of Toxoplasma gondii ME49 to humans through contaminated water and may have...

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Autores principales: Saremy, Sadegh, Boroujeni, Mahdi Eskandarian, Bhattacharjee, Biplab, Mittal, Viditi, Chatterjee, Jhinuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biomedical Informatics 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280436/
https://www.ncbi.nlm.nih.gov/pubmed/22347778
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author Saremy, Sadegh
Boroujeni, Mahdi Eskandarian
Bhattacharjee, Biplab
Mittal, Viditi
Chatterjee, Jhinuk
author_facet Saremy, Sadegh
Boroujeni, Mahdi Eskandarian
Bhattacharjee, Biplab
Mittal, Viditi
Chatterjee, Jhinuk
author_sort Saremy, Sadegh
collection PubMed
description Toxoplasma gondii ME49 is an obligatory intracellular apicomplexa parasite that causes toxoplasmosis in humans, domesticated and wild animals. Waterborne outbreaks of acute toxoplasmosis worldwide reinforce the transmission of Toxoplasma gondii ME49 to humans through contaminated water and may have a greater epidemiological impact than previously believed. In the quest for drug and vaccine target identification subtractive genomics involving subtraction between the host and pathogen genome has been implemented for enlisting essential pathogen specific proteins. Using this approach, our analysis on both human and Toxoplasma gondii ME49 reveals that out of 7987 protein coding sequences of the pathogen, 950 represent essential non human-homologous proteins. Subcellular localization prediction & comparative-biochemical pathway analysis of these essential proteins gives a list of apicoplast-associated proteins having unique pathogen-specific metabolic pathway. These apicoplast-associated enzymes involved in fatty acid biosynthesis pathway of Toxoplasma gondii ME49, may be used as potential drug targets, as the pathway is vital for the protozoan's survival. Structure prediction of drug target proteins was done using fold based recognition method. Screening of the functional inhibitors against these novel targets may result in discovery of novel therapeutic compounds that can be effective against Toxoplasma gondii ME49. ABBREVIATIONS: DEG - Database of Essential Gene, KEGG - Kyoto Encyclopaedia of Genes and Genomes, KAAS - KEGG Automated Annotation Server, PFP - Protein Function Prediction, COG - Cluster of Orthologous Genes.
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spelling pubmed-32804362012-02-17 Identification of potential apicoplast associated therapeutic targets in human and animal pathogen Toxoplasma gondii ME49 Saremy, Sadegh Boroujeni, Mahdi Eskandarian Bhattacharjee, Biplab Mittal, Viditi Chatterjee, Jhinuk Bioinformation Hypothesis Toxoplasma gondii ME49 is an obligatory intracellular apicomplexa parasite that causes toxoplasmosis in humans, domesticated and wild animals. Waterborne outbreaks of acute toxoplasmosis worldwide reinforce the transmission of Toxoplasma gondii ME49 to humans through contaminated water and may have a greater epidemiological impact than previously believed. In the quest for drug and vaccine target identification subtractive genomics involving subtraction between the host and pathogen genome has been implemented for enlisting essential pathogen specific proteins. Using this approach, our analysis on both human and Toxoplasma gondii ME49 reveals that out of 7987 protein coding sequences of the pathogen, 950 represent essential non human-homologous proteins. Subcellular localization prediction & comparative-biochemical pathway analysis of these essential proteins gives a list of apicoplast-associated proteins having unique pathogen-specific metabolic pathway. These apicoplast-associated enzymes involved in fatty acid biosynthesis pathway of Toxoplasma gondii ME49, may be used as potential drug targets, as the pathway is vital for the protozoan's survival. Structure prediction of drug target proteins was done using fold based recognition method. Screening of the functional inhibitors against these novel targets may result in discovery of novel therapeutic compounds that can be effective against Toxoplasma gondii ME49. ABBREVIATIONS: DEG - Database of Essential Gene, KEGG - Kyoto Encyclopaedia of Genes and Genomes, KAAS - KEGG Automated Annotation Server, PFP - Protein Function Prediction, COG - Cluster of Orthologous Genes. Biomedical Informatics 2011-12-21 /pmc/articles/PMC3280436/ /pubmed/22347778 Text en © 2011 Biomedical Informatics This is an open-access article, which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original author and source are credited.
spellingShingle Hypothesis
Saremy, Sadegh
Boroujeni, Mahdi Eskandarian
Bhattacharjee, Biplab
Mittal, Viditi
Chatterjee, Jhinuk
Identification of potential apicoplast associated therapeutic targets in human and animal pathogen Toxoplasma gondii ME49
title Identification of potential apicoplast associated therapeutic targets in human and animal pathogen Toxoplasma gondii ME49
title_full Identification of potential apicoplast associated therapeutic targets in human and animal pathogen Toxoplasma gondii ME49
title_fullStr Identification of potential apicoplast associated therapeutic targets in human and animal pathogen Toxoplasma gondii ME49
title_full_unstemmed Identification of potential apicoplast associated therapeutic targets in human and animal pathogen Toxoplasma gondii ME49
title_short Identification of potential apicoplast associated therapeutic targets in human and animal pathogen Toxoplasma gondii ME49
title_sort identification of potential apicoplast associated therapeutic targets in human and animal pathogen toxoplasma gondii me49
topic Hypothesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280436/
https://www.ncbi.nlm.nih.gov/pubmed/22347778
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