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Analysis of the Genome of the Sexually Transmitted Insect Virus Helicoverpa zea Nudivirus 2

The sexually transmitted insect virus Helicoverpa zea nudivirus 2 (HzNV-2) was determined to have a circular double-stranded DNA genome of 231,621 bp coding for an estimated 113 open reading frames (ORFs). HzNV-2 is most closely related to the nudiviruses, a sister group of the insect baculoviruses....

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Autores principales: Burand, John P., Kim, Woojin, Afonso, Claudio L., Tulman, Edan R., Kutish, Gerald F., Lu, Zhiqiang, Rock, Daniel L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280521/
https://www.ncbi.nlm.nih.gov/pubmed/22355451
http://dx.doi.org/10.3390/v4010028
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author Burand, John P.
Kim, Woojin
Afonso, Claudio L.
Tulman, Edan R.
Kutish, Gerald F.
Lu, Zhiqiang
Rock, Daniel L.
author_facet Burand, John P.
Kim, Woojin
Afonso, Claudio L.
Tulman, Edan R.
Kutish, Gerald F.
Lu, Zhiqiang
Rock, Daniel L.
author_sort Burand, John P.
collection PubMed
description The sexually transmitted insect virus Helicoverpa zea nudivirus 2 (HzNV-2) was determined to have a circular double-stranded DNA genome of 231,621 bp coding for an estimated 113 open reading frames (ORFs). HzNV-2 is most closely related to the nudiviruses, a sister group of the insect baculoviruses. Several putative ORFs that share homology with the baculovirus core genes were identified in the viral genome. However, HzNV-2 lacks several key genetic features of baculoviruses including the late transcriptional regulation factor, LEF-1 and the palindromic hrs, which serve as origins of replication. The HzNV-2 genome was found to code for three ORFs that had significant sequence homology to cellular genes which are not generally found in viral genomes. These included a presumed juvenile hormone esterase gene, a gene coding for a putative zinc-dependent matrix metalloprotease, and a major facilitator superfamily protein gene; all of which are believed to play a role in the cellular proliferation and the tissue hypertrophy observed in the malformation of reproductive organs observed in HzNV-2 infected corn earworm moths, Helicoverpa zea.
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spelling pubmed-32805212012-02-21 Analysis of the Genome of the Sexually Transmitted Insect Virus Helicoverpa zea Nudivirus 2 Burand, John P. Kim, Woojin Afonso, Claudio L. Tulman, Edan R. Kutish, Gerald F. Lu, Zhiqiang Rock, Daniel L. Viruses Article The sexually transmitted insect virus Helicoverpa zea nudivirus 2 (HzNV-2) was determined to have a circular double-stranded DNA genome of 231,621 bp coding for an estimated 113 open reading frames (ORFs). HzNV-2 is most closely related to the nudiviruses, a sister group of the insect baculoviruses. Several putative ORFs that share homology with the baculovirus core genes were identified in the viral genome. However, HzNV-2 lacks several key genetic features of baculoviruses including the late transcriptional regulation factor, LEF-1 and the palindromic hrs, which serve as origins of replication. The HzNV-2 genome was found to code for three ORFs that had significant sequence homology to cellular genes which are not generally found in viral genomes. These included a presumed juvenile hormone esterase gene, a gene coding for a putative zinc-dependent matrix metalloprotease, and a major facilitator superfamily protein gene; all of which are believed to play a role in the cellular proliferation and the tissue hypertrophy observed in the malformation of reproductive organs observed in HzNV-2 infected corn earworm moths, Helicoverpa zea. MDPI 2012-01-06 /pmc/articles/PMC3280521/ /pubmed/22355451 http://dx.doi.org/10.3390/v4010028 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Burand, John P.
Kim, Woojin
Afonso, Claudio L.
Tulman, Edan R.
Kutish, Gerald F.
Lu, Zhiqiang
Rock, Daniel L.
Analysis of the Genome of the Sexually Transmitted Insect Virus Helicoverpa zea Nudivirus 2
title Analysis of the Genome of the Sexually Transmitted Insect Virus Helicoverpa zea Nudivirus 2
title_full Analysis of the Genome of the Sexually Transmitted Insect Virus Helicoverpa zea Nudivirus 2
title_fullStr Analysis of the Genome of the Sexually Transmitted Insect Virus Helicoverpa zea Nudivirus 2
title_full_unstemmed Analysis of the Genome of the Sexually Transmitted Insect Virus Helicoverpa zea Nudivirus 2
title_short Analysis of the Genome of the Sexually Transmitted Insect Virus Helicoverpa zea Nudivirus 2
title_sort analysis of the genome of the sexually transmitted insect virus helicoverpa zea nudivirus 2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280521/
https://www.ncbi.nlm.nih.gov/pubmed/22355451
http://dx.doi.org/10.3390/v4010028
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