Cargando…

Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish

Discovery and development of new antitumor agents from abundant marine fish are attracting an increasing interest. In the present study, we extracted and purified a novel antitumor protein Syngnathusin from the whole body of Syngnathus acus L., a precious marine fish traditionally used for tumors. S...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Mengyue, Nie, Yuxiao, Peng, Ying, He, Fen, Yang, Jingyu, Wu, Chunfu, Li, Xiaobo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280537/
https://www.ncbi.nlm.nih.gov/pubmed/22363219
http://dx.doi.org/10.3390/md10010035
_version_ 1782223843232841728
author Wang, Mengyue
Nie, Yuxiao
Peng, Ying
He, Fen
Yang, Jingyu
Wu, Chunfu
Li, Xiaobo
author_facet Wang, Mengyue
Nie, Yuxiao
Peng, Ying
He, Fen
Yang, Jingyu
Wu, Chunfu
Li, Xiaobo
author_sort Wang, Mengyue
collection PubMed
description Discovery and development of new antitumor agents from abundant marine fish are attracting an increasing interest. In the present study, we extracted and purified a novel antitumor protein Syngnathusin from the whole body of Syngnathus acus L., a precious marine fish traditionally used for tumors. Syngnathusin was comprised of 16 kinds of amino acids, mainly acidic amino acids. Its molecular weight was 67.3 kDa and its isoelectric point was 4.57. The N-terminal amino acid sequence of Syngnathusin was determined to be Lys-Arg-Asp-Leu-Gly-Phe-Val-Asp-Glu-Ile-Ser-Ala-His-Tyr and showed no significant homology with the known proteins. Syngnathusin could significantly inhibit the growth of A549 and CCRF-CEM cells. However, the obvious proliferation inhibition against human non-tumor cell lines was not observed. Flow cytometry, morphologic assessment and comet assay revealed that Syngnathusin could induce apoptosis in A549 and CCRF-CEM cells and strongly cooperated with MTX. Syngnathusin could inhibit the growth of S180 tumor transplanted in mice. Syngnathusin may be developed as a novel, selective and effective antineoplastic agent.
format Online
Article
Text
id pubmed-3280537
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-32805372012-02-23 Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish Wang, Mengyue Nie, Yuxiao Peng, Ying He, Fen Yang, Jingyu Wu, Chunfu Li, Xiaobo Mar Drugs Article Discovery and development of new antitumor agents from abundant marine fish are attracting an increasing interest. In the present study, we extracted and purified a novel antitumor protein Syngnathusin from the whole body of Syngnathus acus L., a precious marine fish traditionally used for tumors. Syngnathusin was comprised of 16 kinds of amino acids, mainly acidic amino acids. Its molecular weight was 67.3 kDa and its isoelectric point was 4.57. The N-terminal amino acid sequence of Syngnathusin was determined to be Lys-Arg-Asp-Leu-Gly-Phe-Val-Asp-Glu-Ile-Ser-Ala-His-Tyr and showed no significant homology with the known proteins. Syngnathusin could significantly inhibit the growth of A549 and CCRF-CEM cells. However, the obvious proliferation inhibition against human non-tumor cell lines was not observed. Flow cytometry, morphologic assessment and comet assay revealed that Syngnathusin could induce apoptosis in A549 and CCRF-CEM cells and strongly cooperated with MTX. Syngnathusin could inhibit the growth of S180 tumor transplanted in mice. Syngnathusin may be developed as a novel, selective and effective antineoplastic agent. MDPI 2011-12-30 /pmc/articles/PMC3280537/ /pubmed/22363219 http://dx.doi.org/10.3390/md10010035 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Mengyue
Nie, Yuxiao
Peng, Ying
He, Fen
Yang, Jingyu
Wu, Chunfu
Li, Xiaobo
Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish
title Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish
title_full Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish
title_fullStr Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish
title_full_unstemmed Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish
title_short Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish
title_sort purification, characterization and antitumor activities of a new protein from syngnathus acus, an officinal marine fish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280537/
https://www.ncbi.nlm.nih.gov/pubmed/22363219
http://dx.doi.org/10.3390/md10010035
work_keys_str_mv AT wangmengyue purificationcharacterizationandantitumoractivitiesofanewproteinfromsyngnathusacusanofficinalmarinefish
AT nieyuxiao purificationcharacterizationandantitumoractivitiesofanewproteinfromsyngnathusacusanofficinalmarinefish
AT pengying purificationcharacterizationandantitumoractivitiesofanewproteinfromsyngnathusacusanofficinalmarinefish
AT hefen purificationcharacterizationandantitumoractivitiesofanewproteinfromsyngnathusacusanofficinalmarinefish
AT yangjingyu purificationcharacterizationandantitumoractivitiesofanewproteinfromsyngnathusacusanofficinalmarinefish
AT wuchunfu purificationcharacterizationandantitumoractivitiesofanewproteinfromsyngnathusacusanofficinalmarinefish
AT lixiaobo purificationcharacterizationandantitumoractivitiesofanewproteinfromsyngnathusacusanofficinalmarinefish