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Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish
Discovery and development of new antitumor agents from abundant marine fish are attracting an increasing interest. In the present study, we extracted and purified a novel antitumor protein Syngnathusin from the whole body of Syngnathus acus L., a precious marine fish traditionally used for tumors. S...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280537/ https://www.ncbi.nlm.nih.gov/pubmed/22363219 http://dx.doi.org/10.3390/md10010035 |
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author | Wang, Mengyue Nie, Yuxiao Peng, Ying He, Fen Yang, Jingyu Wu, Chunfu Li, Xiaobo |
author_facet | Wang, Mengyue Nie, Yuxiao Peng, Ying He, Fen Yang, Jingyu Wu, Chunfu Li, Xiaobo |
author_sort | Wang, Mengyue |
collection | PubMed |
description | Discovery and development of new antitumor agents from abundant marine fish are attracting an increasing interest. In the present study, we extracted and purified a novel antitumor protein Syngnathusin from the whole body of Syngnathus acus L., a precious marine fish traditionally used for tumors. Syngnathusin was comprised of 16 kinds of amino acids, mainly acidic amino acids. Its molecular weight was 67.3 kDa and its isoelectric point was 4.57. The N-terminal amino acid sequence of Syngnathusin was determined to be Lys-Arg-Asp-Leu-Gly-Phe-Val-Asp-Glu-Ile-Ser-Ala-His-Tyr and showed no significant homology with the known proteins. Syngnathusin could significantly inhibit the growth of A549 and CCRF-CEM cells. However, the obvious proliferation inhibition against human non-tumor cell lines was not observed. Flow cytometry, morphologic assessment and comet assay revealed that Syngnathusin could induce apoptosis in A549 and CCRF-CEM cells and strongly cooperated with MTX. Syngnathusin could inhibit the growth of S180 tumor transplanted in mice. Syngnathusin may be developed as a novel, selective and effective antineoplastic agent. |
format | Online Article Text |
id | pubmed-3280537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-32805372012-02-23 Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish Wang, Mengyue Nie, Yuxiao Peng, Ying He, Fen Yang, Jingyu Wu, Chunfu Li, Xiaobo Mar Drugs Article Discovery and development of new antitumor agents from abundant marine fish are attracting an increasing interest. In the present study, we extracted and purified a novel antitumor protein Syngnathusin from the whole body of Syngnathus acus L., a precious marine fish traditionally used for tumors. Syngnathusin was comprised of 16 kinds of amino acids, mainly acidic amino acids. Its molecular weight was 67.3 kDa and its isoelectric point was 4.57. The N-terminal amino acid sequence of Syngnathusin was determined to be Lys-Arg-Asp-Leu-Gly-Phe-Val-Asp-Glu-Ile-Ser-Ala-His-Tyr and showed no significant homology with the known proteins. Syngnathusin could significantly inhibit the growth of A549 and CCRF-CEM cells. However, the obvious proliferation inhibition against human non-tumor cell lines was not observed. Flow cytometry, morphologic assessment and comet assay revealed that Syngnathusin could induce apoptosis in A549 and CCRF-CEM cells and strongly cooperated with MTX. Syngnathusin could inhibit the growth of S180 tumor transplanted in mice. Syngnathusin may be developed as a novel, selective and effective antineoplastic agent. MDPI 2011-12-30 /pmc/articles/PMC3280537/ /pubmed/22363219 http://dx.doi.org/10.3390/md10010035 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Wang, Mengyue Nie, Yuxiao Peng, Ying He, Fen Yang, Jingyu Wu, Chunfu Li, Xiaobo Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish |
title | Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish |
title_full | Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish |
title_fullStr | Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish |
title_full_unstemmed | Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish |
title_short | Purification, Characterization and Antitumor Activities of a New Protein from Syngnathus acus, an Officinal Marine Fish |
title_sort | purification, characterization and antitumor activities of a new protein from syngnathus acus, an officinal marine fish |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280537/ https://www.ncbi.nlm.nih.gov/pubmed/22363219 http://dx.doi.org/10.3390/md10010035 |
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