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Transgenic strategy for identifying synaptic connections in mice by fluorescence complementation (GRASP)
In the “GFP reconstitution across synaptic partners” (GRASP) method, non-fluorescent fragments of GFP are expressed in two different neurons; the fragments self-assemble at synapses between the two to form a fluorophore. GRASP has proven useful for light microscopic identification of synapses in two...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280602/ https://www.ncbi.nlm.nih.gov/pubmed/22355283 http://dx.doi.org/10.3389/fnmol.2012.00018 |