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Borrelia burgdorferi Requires the Alternative Sigma Factor RpoS for Dissemination within the Vector during Tick-to-Mammal Transmission

While the roles of rpoS(Bb) and RpoS-dependent genes have been studied extensively within the mammal, the contribution of the RpoS regulon to the tick-phase of the Borrelia burgdorferi enzootic cycle has not been examined. Herein, we demonstrate that RpoS-dependent gene expression is prerequisite fo...

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Autores principales: Dunham-Ems, Star M., Caimano, Melissa J., Eggers, Christian H., Radolf, Justin D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280991/
https://www.ncbi.nlm.nih.gov/pubmed/22359504
http://dx.doi.org/10.1371/journal.ppat.1002532
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author Dunham-Ems, Star M.
Caimano, Melissa J.
Eggers, Christian H.
Radolf, Justin D.
author_facet Dunham-Ems, Star M.
Caimano, Melissa J.
Eggers, Christian H.
Radolf, Justin D.
author_sort Dunham-Ems, Star M.
collection PubMed
description While the roles of rpoS(Bb) and RpoS-dependent genes have been studied extensively within the mammal, the contribution of the RpoS regulon to the tick-phase of the Borrelia burgdorferi enzootic cycle has not been examined. Herein, we demonstrate that RpoS-dependent gene expression is prerequisite for the transmission of spirochetes by feeding nymphs. RpoS-deficient organisms are confined to the midgut lumen where they transform into an unusual morphotype (round bodies) during the later stages of the blood meal. We show that round body formation is rapidly reversible, and in vitro appears to be attributable, in part, to reduced levels of Coenzyme A disulfide reductase, which among other functions, provides NAD(+) for glycolysis. Our data suggest that spirochetes default to an RpoS-independent program for round body formation upon sensing that the energetics for transmission are unfavorable.
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spelling pubmed-32809912012-02-22 Borrelia burgdorferi Requires the Alternative Sigma Factor RpoS for Dissemination within the Vector during Tick-to-Mammal Transmission Dunham-Ems, Star M. Caimano, Melissa J. Eggers, Christian H. Radolf, Justin D. PLoS Pathog Research Article While the roles of rpoS(Bb) and RpoS-dependent genes have been studied extensively within the mammal, the contribution of the RpoS regulon to the tick-phase of the Borrelia burgdorferi enzootic cycle has not been examined. Herein, we demonstrate that RpoS-dependent gene expression is prerequisite for the transmission of spirochetes by feeding nymphs. RpoS-deficient organisms are confined to the midgut lumen where they transform into an unusual morphotype (round bodies) during the later stages of the blood meal. We show that round body formation is rapidly reversible, and in vitro appears to be attributable, in part, to reduced levels of Coenzyme A disulfide reductase, which among other functions, provides NAD(+) for glycolysis. Our data suggest that spirochetes default to an RpoS-independent program for round body formation upon sensing that the energetics for transmission are unfavorable. Public Library of Science 2012-02-16 /pmc/articles/PMC3280991/ /pubmed/22359504 http://dx.doi.org/10.1371/journal.ppat.1002532 Text en Dunham-Ems et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dunham-Ems, Star M.
Caimano, Melissa J.
Eggers, Christian H.
Radolf, Justin D.
Borrelia burgdorferi Requires the Alternative Sigma Factor RpoS for Dissemination within the Vector during Tick-to-Mammal Transmission
title Borrelia burgdorferi Requires the Alternative Sigma Factor RpoS for Dissemination within the Vector during Tick-to-Mammal Transmission
title_full Borrelia burgdorferi Requires the Alternative Sigma Factor RpoS for Dissemination within the Vector during Tick-to-Mammal Transmission
title_fullStr Borrelia burgdorferi Requires the Alternative Sigma Factor RpoS for Dissemination within the Vector during Tick-to-Mammal Transmission
title_full_unstemmed Borrelia burgdorferi Requires the Alternative Sigma Factor RpoS for Dissemination within the Vector during Tick-to-Mammal Transmission
title_short Borrelia burgdorferi Requires the Alternative Sigma Factor RpoS for Dissemination within the Vector during Tick-to-Mammal Transmission
title_sort borrelia burgdorferi requires the alternative sigma factor rpos for dissemination within the vector during tick-to-mammal transmission
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280991/
https://www.ncbi.nlm.nih.gov/pubmed/22359504
http://dx.doi.org/10.1371/journal.ppat.1002532
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