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ATP-Independent Cooperative Binding of Yeast Isw1a to Bare and Nucleosomal DNA

Among chromatin remodeling factors, the ISWI family displays a nucleosome-enhanced ATPase activity coupled to DNA translocation. While these enzymes are known to bind to DNA, their activity has not been fully characterized. Here we use TEM imaging and single molecule manipulation to investigate the...

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Autores principales: De Cian, Anne, Praly, Elise, Ding, Fangyuan, Singh, Vijender, Lavelle, Christophe, Le Cam, Eric, Croquette, Vincent, Piétrement, Olivier, Bensimon, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281020/
https://www.ncbi.nlm.nih.gov/pubmed/22359636
http://dx.doi.org/10.1371/journal.pone.0031845
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author De Cian, Anne
Praly, Elise
Ding, Fangyuan
Singh, Vijender
Lavelle, Christophe
Le Cam, Eric
Croquette, Vincent
Piétrement, Olivier
Bensimon, David
author_facet De Cian, Anne
Praly, Elise
Ding, Fangyuan
Singh, Vijender
Lavelle, Christophe
Le Cam, Eric
Croquette, Vincent
Piétrement, Olivier
Bensimon, David
author_sort De Cian, Anne
collection PubMed
description Among chromatin remodeling factors, the ISWI family displays a nucleosome-enhanced ATPase activity coupled to DNA translocation. While these enzymes are known to bind to DNA, their activity has not been fully characterized. Here we use TEM imaging and single molecule manipulation to investigate the interaction between DNA and yeast Isw1a. We show that Isw1a displays a highly cooperative ATP-independent binding to and bridging between DNA segments. Under appropriate tension, rare single nucleation events can sometimes be observed and loop DNA with a regular step. These nucleation events are often followed by binding of successive complexes bridging between nearby DNA segments in a zipper-like fashion, as confirmed by TEM observations. On nucleosomal substrates, we show that the specific ATP-dependent remodeling activity occurs in the context of cooperative Isw1a complexes bridging extranucleosomal DNA. Our results are interpreted in the context of the recently published partial structure of Isw1a and support its acting as a “protein ruler” (with possibly more than one tick).
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spelling pubmed-32810202012-02-22 ATP-Independent Cooperative Binding of Yeast Isw1a to Bare and Nucleosomal DNA De Cian, Anne Praly, Elise Ding, Fangyuan Singh, Vijender Lavelle, Christophe Le Cam, Eric Croquette, Vincent Piétrement, Olivier Bensimon, David PLoS One Research Article Among chromatin remodeling factors, the ISWI family displays a nucleosome-enhanced ATPase activity coupled to DNA translocation. While these enzymes are known to bind to DNA, their activity has not been fully characterized. Here we use TEM imaging and single molecule manipulation to investigate the interaction between DNA and yeast Isw1a. We show that Isw1a displays a highly cooperative ATP-independent binding to and bridging between DNA segments. Under appropriate tension, rare single nucleation events can sometimes be observed and loop DNA with a regular step. These nucleation events are often followed by binding of successive complexes bridging between nearby DNA segments in a zipper-like fashion, as confirmed by TEM observations. On nucleosomal substrates, we show that the specific ATP-dependent remodeling activity occurs in the context of cooperative Isw1a complexes bridging extranucleosomal DNA. Our results are interpreted in the context of the recently published partial structure of Isw1a and support its acting as a “protein ruler” (with possibly more than one tick). Public Library of Science 2012-02-16 /pmc/articles/PMC3281020/ /pubmed/22359636 http://dx.doi.org/10.1371/journal.pone.0031845 Text en De Cian et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
De Cian, Anne
Praly, Elise
Ding, Fangyuan
Singh, Vijender
Lavelle, Christophe
Le Cam, Eric
Croquette, Vincent
Piétrement, Olivier
Bensimon, David
ATP-Independent Cooperative Binding of Yeast Isw1a to Bare and Nucleosomal DNA
title ATP-Independent Cooperative Binding of Yeast Isw1a to Bare and Nucleosomal DNA
title_full ATP-Independent Cooperative Binding of Yeast Isw1a to Bare and Nucleosomal DNA
title_fullStr ATP-Independent Cooperative Binding of Yeast Isw1a to Bare and Nucleosomal DNA
title_full_unstemmed ATP-Independent Cooperative Binding of Yeast Isw1a to Bare and Nucleosomal DNA
title_short ATP-Independent Cooperative Binding of Yeast Isw1a to Bare and Nucleosomal DNA
title_sort atp-independent cooperative binding of yeast isw1a to bare and nucleosomal dna
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281020/
https://www.ncbi.nlm.nih.gov/pubmed/22359636
http://dx.doi.org/10.1371/journal.pone.0031845
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