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Small Fragment Homologous Replacement: Evaluation of Factors Influencing Modification Efficiency in an Eukaryotic Assay System

Homologous Replacement is used to modify specific gene sequences of chromosomal DNA in a process referred to as “Small Fragment Homologous Replacement”, where DNA fragments replace genomic target resulting in specific sequence changes. To optimize the efficiency of this process, we developed a repor...

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Detalles Bibliográficos
Autores principales:	Luchetti, Andrea, Filareto, Antonio, Sanchez, Massimo, Ferraguti, Giampiero, Lucarelli, Marco, Novelli, Giuseppe, Sangiuolo, Federica, Malgieri, Arianna
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281040/ https://www.ncbi.nlm.nih.gov/pubmed/22359552 http://dx.doi.org/10.1371/journal.pone.0030851

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