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C. elegans VANG-1 Modulates Life Span via Insulin/IGF-1-Like Signaling

The planar cell polarity (PCP) pathway is highly conserved from Drosophila to humans and a PCP-like pathway has recently been described in the nematode Caenorhabditis elegans. The developmental function of this pathway is to coordinate the orientation of cells or structures within the plane of an ep...

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Autores principales: Honnen, Sebastian J., Büchter, Christian, Schröder, Verena, Hoffmann, Michael, Kohara, Yuji, Kampkötter, Andreas, Bossinger, Olaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281126/
https://www.ncbi.nlm.nih.gov/pubmed/22359667
http://dx.doi.org/10.1371/journal.pone.0032183
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author Honnen, Sebastian J.
Büchter, Christian
Schröder, Verena
Hoffmann, Michael
Kohara, Yuji
Kampkötter, Andreas
Bossinger, Olaf
author_facet Honnen, Sebastian J.
Büchter, Christian
Schröder, Verena
Hoffmann, Michael
Kohara, Yuji
Kampkötter, Andreas
Bossinger, Olaf
author_sort Honnen, Sebastian J.
collection PubMed
description The planar cell polarity (PCP) pathway is highly conserved from Drosophila to humans and a PCP-like pathway has recently been described in the nematode Caenorhabditis elegans. The developmental function of this pathway is to coordinate the orientation of cells or structures within the plane of an epithelium or to organize cell-cell intercalation required for correct morphogenesis. Here, we describe a novel role of VANG-1, the only C. elegans ortholog of the conserved PCP component Strabismus/Van Gogh. We show that two alleles of vang-1 and depletion of the protein by RNAi cause an increase of mean life span up to 40%. Consistent with the longevity phenotype vang-1 animals also show enhanced resistance to thermal- and oxidative stress and decreased lipofuscin accumulation. In addition, vang-1 mutants show defects like reduced brood size, decreased ovulation rate and prolonged reproductive span, which are also related to gerontogenes. The germline, but not the intestine or neurons, seems to be the primary site of vang-1 function. Life span extension in vang-1 mutants depends on the insulin/IGF-1-like receptor DAF-2 and DAF-16/FoxO transcription factor. RNAi against the phase II detoxification transcription factor SKN-1/Nrf2 also reduced vang-1 life span that might be explained by gradual inhibition of insulin/IGF-1-like signaling in vang-1. This is the first time that a key player of the PCP pathway is shown to be involved in the insulin/IGF-1-like signaling dependent modulation of life span in C. elegans.
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spelling pubmed-32811262012-02-22 C. elegans VANG-1 Modulates Life Span via Insulin/IGF-1-Like Signaling Honnen, Sebastian J. Büchter, Christian Schröder, Verena Hoffmann, Michael Kohara, Yuji Kampkötter, Andreas Bossinger, Olaf PLoS One Research Article The planar cell polarity (PCP) pathway is highly conserved from Drosophila to humans and a PCP-like pathway has recently been described in the nematode Caenorhabditis elegans. The developmental function of this pathway is to coordinate the orientation of cells or structures within the plane of an epithelium or to organize cell-cell intercalation required for correct morphogenesis. Here, we describe a novel role of VANG-1, the only C. elegans ortholog of the conserved PCP component Strabismus/Van Gogh. We show that two alleles of vang-1 and depletion of the protein by RNAi cause an increase of mean life span up to 40%. Consistent with the longevity phenotype vang-1 animals also show enhanced resistance to thermal- and oxidative stress and decreased lipofuscin accumulation. In addition, vang-1 mutants show defects like reduced brood size, decreased ovulation rate and prolonged reproductive span, which are also related to gerontogenes. The germline, but not the intestine or neurons, seems to be the primary site of vang-1 function. Life span extension in vang-1 mutants depends on the insulin/IGF-1-like receptor DAF-2 and DAF-16/FoxO transcription factor. RNAi against the phase II detoxification transcription factor SKN-1/Nrf2 also reduced vang-1 life span that might be explained by gradual inhibition of insulin/IGF-1-like signaling in vang-1. This is the first time that a key player of the PCP pathway is shown to be involved in the insulin/IGF-1-like signaling dependent modulation of life span in C. elegans. Public Library of Science 2012-02-16 /pmc/articles/PMC3281126/ /pubmed/22359667 http://dx.doi.org/10.1371/journal.pone.0032183 Text en Honnen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Honnen, Sebastian J.
Büchter, Christian
Schröder, Verena
Hoffmann, Michael
Kohara, Yuji
Kampkötter, Andreas
Bossinger, Olaf
C. elegans VANG-1 Modulates Life Span via Insulin/IGF-1-Like Signaling
title C. elegans VANG-1 Modulates Life Span via Insulin/IGF-1-Like Signaling
title_full C. elegans VANG-1 Modulates Life Span via Insulin/IGF-1-Like Signaling
title_fullStr C. elegans VANG-1 Modulates Life Span via Insulin/IGF-1-Like Signaling
title_full_unstemmed C. elegans VANG-1 Modulates Life Span via Insulin/IGF-1-Like Signaling
title_short C. elegans VANG-1 Modulates Life Span via Insulin/IGF-1-Like Signaling
title_sort c. elegans vang-1 modulates life span via insulin/igf-1-like signaling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281126/
https://www.ncbi.nlm.nih.gov/pubmed/22359667
http://dx.doi.org/10.1371/journal.pone.0032183
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