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The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
Phosphate activation of the mitochondrial permeability transition pore (MPTP) opening is well-documented and could involve the phosphate carrier (PiC) that we have proposed is the pore's cyclophilin-D binding component. However, others have reported that following CyP-D ablation Pi inhibits MPT...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281194/ https://www.ncbi.nlm.nih.gov/pubmed/21586347 http://dx.doi.org/10.1016/j.mito.2011.04.006 |
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author | Varanyuwatana, Pinadda Halestrap, Andrew P. |
author_facet | Varanyuwatana, Pinadda Halestrap, Andrew P. |
author_sort | Varanyuwatana, Pinadda |
collection | PubMed |
description | Phosphate activation of the mitochondrial permeability transition pore (MPTP) opening is well-documented and could involve the phosphate carrier (PiC) that we have proposed is the pore's cyclophilin-D binding component. However, others have reported that following CyP-D ablation Pi inhibits MPTP opening while cyclosporine-A (CsA) inhibits MPTP opening only when Pi is present. Here we demonstrate that Pi activates MPTP opening under all energised and de-energised conditions tested while CsA inhibits pore opening whether or not Pi is present. Using siRNA in HeLa cells we could reduce PiC expression by 65–80% but this inhibited neither mitochondrial calcium accumulation nor MPTP opening. |
format | Online Article Text |
id | pubmed-3281194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Elsevier Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32811942012-02-24 The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore Varanyuwatana, Pinadda Halestrap, Andrew P. Mitochondrion Article Phosphate activation of the mitochondrial permeability transition pore (MPTP) opening is well-documented and could involve the phosphate carrier (PiC) that we have proposed is the pore's cyclophilin-D binding component. However, others have reported that following CyP-D ablation Pi inhibits MPTP opening while cyclosporine-A (CsA) inhibits MPTP opening only when Pi is present. Here we demonstrate that Pi activates MPTP opening under all energised and de-energised conditions tested while CsA inhibits pore opening whether or not Pi is present. Using siRNA in HeLa cells we could reduce PiC expression by 65–80% but this inhibited neither mitochondrial calcium accumulation nor MPTP opening. Elsevier Science 2012-01 /pmc/articles/PMC3281194/ /pubmed/21586347 http://dx.doi.org/10.1016/j.mito.2011.04.006 Text en © 2012 Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license |
spellingShingle | Article Varanyuwatana, Pinadda Halestrap, Andrew P. The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore |
title | The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore |
title_full | The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore |
title_fullStr | The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore |
title_full_unstemmed | The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore |
title_short | The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore |
title_sort | roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281194/ https://www.ncbi.nlm.nih.gov/pubmed/21586347 http://dx.doi.org/10.1016/j.mito.2011.04.006 |
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