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The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore

Phosphate activation of the mitochondrial permeability transition pore (MPTP) opening is well-documented and could involve the phosphate carrier (PiC) that we have proposed is the pore's cyclophilin-D binding component. However, others have reported that following CyP-D ablation Pi inhibits MPT...

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Detalles Bibliográficos
Autores principales: Varanyuwatana, Pinadda, Halestrap, Andrew P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281194/
https://www.ncbi.nlm.nih.gov/pubmed/21586347
http://dx.doi.org/10.1016/j.mito.2011.04.006
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author Varanyuwatana, Pinadda
Halestrap, Andrew P.
author_facet Varanyuwatana, Pinadda
Halestrap, Andrew P.
author_sort Varanyuwatana, Pinadda
collection PubMed
description Phosphate activation of the mitochondrial permeability transition pore (MPTP) opening is well-documented and could involve the phosphate carrier (PiC) that we have proposed is the pore's cyclophilin-D binding component. However, others have reported that following CyP-D ablation Pi inhibits MPTP opening while cyclosporine-A (CsA) inhibits MPTP opening only when Pi is present. Here we demonstrate that Pi activates MPTP opening under all energised and de-energised conditions tested while CsA inhibits pore opening whether or not Pi is present. Using siRNA in HeLa cells we could reduce PiC expression by 65–80% but this inhibited neither mitochondrial calcium accumulation nor MPTP opening.
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spelling pubmed-32811942012-02-24 The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore Varanyuwatana, Pinadda Halestrap, Andrew P. Mitochondrion Article Phosphate activation of the mitochondrial permeability transition pore (MPTP) opening is well-documented and could involve the phosphate carrier (PiC) that we have proposed is the pore's cyclophilin-D binding component. However, others have reported that following CyP-D ablation Pi inhibits MPTP opening while cyclosporine-A (CsA) inhibits MPTP opening only when Pi is present. Here we demonstrate that Pi activates MPTP opening under all energised and de-energised conditions tested while CsA inhibits pore opening whether or not Pi is present. Using siRNA in HeLa cells we could reduce PiC expression by 65–80% but this inhibited neither mitochondrial calcium accumulation nor MPTP opening. Elsevier Science 2012-01 /pmc/articles/PMC3281194/ /pubmed/21586347 http://dx.doi.org/10.1016/j.mito.2011.04.006 Text en © 2012 Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license
spellingShingle Article
Varanyuwatana, Pinadda
Halestrap, Andrew P.
The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
title The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
title_full The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
title_fullStr The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
title_full_unstemmed The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
title_short The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
title_sort roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281194/
https://www.ncbi.nlm.nih.gov/pubmed/21586347
http://dx.doi.org/10.1016/j.mito.2011.04.006
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