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Cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the BMP-2 pathway

INTRODUCTION: Mechanical forces play critical roles in the development and remodelling process of bone. As an alternative cell source for bone engineering, adipose-derived stem cells (ASCs) should be fully investigated for their responses to mechanical stress and the mechanisms responsible for osteo...

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Autores principales: Yang, Xingmei, Gong, Ping, Lin, Yunfeng, Zhang, Lirong, Li, Xiaoyu, Yuan, Quan, Tan, Zhen, Wang, Yongyue, Man, Yi, Tang, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281333/
https://www.ncbi.nlm.nih.gov/pubmed/22371739
http://dx.doi.org/10.5114/aoms.2010.13886
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author Yang, Xingmei
Gong, Ping
Lin, Yunfeng
Zhang, Lirong
Li, Xiaoyu
Yuan, Quan
Tan, Zhen
Wang, Yongyue
Man, Yi
Tang, Hua
author_facet Yang, Xingmei
Gong, Ping
Lin, Yunfeng
Zhang, Lirong
Li, Xiaoyu
Yuan, Quan
Tan, Zhen
Wang, Yongyue
Man, Yi
Tang, Hua
author_sort Yang, Xingmei
collection PubMed
description INTRODUCTION: Mechanical forces play critical roles in the development and remodelling process of bone. As an alternative cell source for bone engineering, adipose-derived stem cells (ASCs) should be fully investigated for their responses to mechanical stress and the mechanisms responsible for osteogenic induction in response to mechanical signals. MATERIAL AND METHODS: We hypothesized that appropriate application of uniaxial cyclic tensile strain to ASCs could increase bone morphogenetic protein-2 (BMP-2) expression and improve osteogenesis of ASCs. To test our hypothesis, ASCs from the same flask of the same donor were subjected to tensile strain with different patterns in order to eliminate the difference of donor site and passage. After surface markers investigation, the osteo-induced ASCs were subjected to uniaxial cyclic tensile stretch with the following two loading patterns: long duration continuous pattern (6 h, 1 HZ, 2000 µɛ) and short duration consecutive pattern (17 min every day for 10 consecutive days, 1 HZ, 2000 µɛ). Then osteogenic related genes were analysed by real-time PCR. RESULTS: The ASCs were positive for the markers STRO-1, CD90 and CD44 and negative for CD34. Cyclic tensile strain of 6 continuous h’ duration significantly increased gene expressions of BMP-2 and Runx2, and depressed OCN mRNA expression. In contrast, mechanical loading of 17 min every day did not significantly affect gene expression of BMP-2, Runx2, OCN or ALP. CONCLUSIONS: We indicate that ASCs may sense mechanical loading in a duration-dependent manner and cyclic tensile stretch may modulate the osteogenic differentiation of ASCs via the BMP-2 signalling pathway.
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spelling pubmed-32813332012-02-27 Cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the BMP-2 pathway Yang, Xingmei Gong, Ping Lin, Yunfeng Zhang, Lirong Li, Xiaoyu Yuan, Quan Tan, Zhen Wang, Yongyue Man, Yi Tang, Hua Arch Med Sci Basic Research INTRODUCTION: Mechanical forces play critical roles in the development and remodelling process of bone. As an alternative cell source for bone engineering, adipose-derived stem cells (ASCs) should be fully investigated for their responses to mechanical stress and the mechanisms responsible for osteogenic induction in response to mechanical signals. MATERIAL AND METHODS: We hypothesized that appropriate application of uniaxial cyclic tensile strain to ASCs could increase bone morphogenetic protein-2 (BMP-2) expression and improve osteogenesis of ASCs. To test our hypothesis, ASCs from the same flask of the same donor were subjected to tensile strain with different patterns in order to eliminate the difference of donor site and passage. After surface markers investigation, the osteo-induced ASCs were subjected to uniaxial cyclic tensile stretch with the following two loading patterns: long duration continuous pattern (6 h, 1 HZ, 2000 µɛ) and short duration consecutive pattern (17 min every day for 10 consecutive days, 1 HZ, 2000 µɛ). Then osteogenic related genes were analysed by real-time PCR. RESULTS: The ASCs were positive for the markers STRO-1, CD90 and CD44 and negative for CD34. Cyclic tensile strain of 6 continuous h’ duration significantly increased gene expressions of BMP-2 and Runx2, and depressed OCN mRNA expression. In contrast, mechanical loading of 17 min every day did not significantly affect gene expression of BMP-2, Runx2, OCN or ALP. CONCLUSIONS: We indicate that ASCs may sense mechanical loading in a duration-dependent manner and cyclic tensile stretch may modulate the osteogenic differentiation of ASCs via the BMP-2 signalling pathway. Termedia Publishing House 2010-04-30 2010-04-30 /pmc/articles/PMC3281333/ /pubmed/22371739 http://dx.doi.org/10.5114/aoms.2010.13886 Text en Copyright © 2010 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Yang, Xingmei
Gong, Ping
Lin, Yunfeng
Zhang, Lirong
Li, Xiaoyu
Yuan, Quan
Tan, Zhen
Wang, Yongyue
Man, Yi
Tang, Hua
Cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the BMP-2 pathway
title Cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the BMP-2 pathway
title_full Cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the BMP-2 pathway
title_fullStr Cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the BMP-2 pathway
title_full_unstemmed Cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the BMP-2 pathway
title_short Cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the BMP-2 pathway
title_sort cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the bmp-2 pathway
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281333/
https://www.ncbi.nlm.nih.gov/pubmed/22371739
http://dx.doi.org/10.5114/aoms.2010.13886
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