Using transcriptome sequencing to identify mechanisms of drug action and resistance

Determining mechanisms of drug action in human cells remains a major challenge. Here we describe an approach in which multiple drug resistant clones are isolated and transcriptome sequencing is used to find mutations in each clone. Further analysis of mutations common to more than one clone can iden...

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Detalles Bibliográficos
Autores principales: Wacker, Sarah A., Houghtaling, Benjamin R., Elemento, Olivier, Kapoor, Tarun M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281560/
https://www.ncbi.nlm.nih.gov/pubmed/22327403
http://dx.doi.org/10.1038/nchembio.779
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author Wacker, Sarah A.
Houghtaling, Benjamin R.
Elemento, Olivier
Kapoor, Tarun M.
author_facet Wacker, Sarah A.
Houghtaling, Benjamin R.
Elemento, Olivier
Kapoor, Tarun M.
author_sort Wacker, Sarah A.
collection PubMed
description Determining mechanisms of drug action in human cells remains a major challenge. Here we describe an approach in which multiple drug resistant clones are isolated and transcriptome sequencing is used to find mutations in each clone. Further analysis of mutations common to more than one clone can identify a drug’s physiological target and indirect resistance mechanisms, as indicated by our proof-of-concept studies of cytotoxic anti-cancer drugs, BI 2536 and bortezomib.
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spelling pubmed-32815602012-09-01 Using transcriptome sequencing to identify mechanisms of drug action and resistance Wacker, Sarah A. Houghtaling, Benjamin R. Elemento, Olivier Kapoor, Tarun M. Nat Chem Biol Article Determining mechanisms of drug action in human cells remains a major challenge. Here we describe an approach in which multiple drug resistant clones are isolated and transcriptome sequencing is used to find mutations in each clone. Further analysis of mutations common to more than one clone can identify a drug’s physiological target and indirect resistance mechanisms, as indicated by our proof-of-concept studies of cytotoxic anti-cancer drugs, BI 2536 and bortezomib. 2012-02-12 /pmc/articles/PMC3281560/ /pubmed/22327403 http://dx.doi.org/10.1038/nchembio.779 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wacker, Sarah A.
Houghtaling, Benjamin R.
Elemento, Olivier
Kapoor, Tarun M.
Using transcriptome sequencing to identify mechanisms of drug action and resistance
title Using transcriptome sequencing to identify mechanisms of drug action and resistance
title_full Using transcriptome sequencing to identify mechanisms of drug action and resistance
title_fullStr Using transcriptome sequencing to identify mechanisms of drug action and resistance
title_full_unstemmed Using transcriptome sequencing to identify mechanisms of drug action and resistance
title_short Using transcriptome sequencing to identify mechanisms of drug action and resistance
title_sort using transcriptome sequencing to identify mechanisms of drug action and resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281560/
https://www.ncbi.nlm.nih.gov/pubmed/22327403
http://dx.doi.org/10.1038/nchembio.779
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