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Hospital and Community Ampicillin-Resistant Enterococcus faecium Are Evolutionarily Closely Linked but Have Diversified through Niche Adaptation

BACKGROUND: Ampicillin-resistant Enterococcus faecium (ARE) has emerged as a nosocomial pathogen. Here, we quantified ARE carriage in different community sources and determined genetic relatedness with hospital ARE. METHODS AND RESULTS: ARE was recovered from rectal swabs of 24 of 79 (30%) dogs, 11...

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Autores principales: de Regt, Marieke J. A., van Schaik, Willem, van Luit-Asbroek, Miranda, Dekker, Huberta A. T., van Duijkeren, Engeline, Koning, Catherina J. M., Bonten, Marc J. M., Willems, Rob J. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281830/
https://www.ncbi.nlm.nih.gov/pubmed/22363425
http://dx.doi.org/10.1371/journal.pone.0030319
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author de Regt, Marieke J. A.
van Schaik, Willem
van Luit-Asbroek, Miranda
Dekker, Huberta A. T.
van Duijkeren, Engeline
Koning, Catherina J. M.
Bonten, Marc J. M.
Willems, Rob J. L.
author_facet de Regt, Marieke J. A.
van Schaik, Willem
van Luit-Asbroek, Miranda
Dekker, Huberta A. T.
van Duijkeren, Engeline
Koning, Catherina J. M.
Bonten, Marc J. M.
Willems, Rob J. L.
author_sort de Regt, Marieke J. A.
collection PubMed
description BACKGROUND: Ampicillin-resistant Enterococcus faecium (ARE) has emerged as a nosocomial pathogen. Here, we quantified ARE carriage in different community sources and determined genetic relatedness with hospital ARE. METHODS AND RESULTS: ARE was recovered from rectal swabs of 24 of 79 (30%) dogs, 11 of 85 (13%) cats and 0 of 42 horses and from 3 of 40 (8%) faecal samples of non-hospitalized humans receiving amoxicillin. Multi-locus Sequence Typing revealed 21 sequence types (STs), including 5 STs frequently associated with hospital-acquired infections. Genes previously found to be enriched in hospital ARE, such as IS16, orf903, orf905, orf907, were highly prevalent in community ARE (≥79%), while genes with a proposed role in pathogenesis, such as esp, hyl and ecbA, were found rarely (≤5%) in community isolates. Comparative genome analysis of 2 representative dog isolates revealed that the dog strain of ST192 was evolutionarily closely linked to two previously sequenced hospital ARE, but had, based on gene content, more genes in common with the other, evolutionarily more distantly related, dog strain (ST266). CONCLUSION: ARE were detected in dogs, cats and sporadically in healthy humans, with evolutionary linkage to hospital ARE. Yet, their accessory genome has diversified, probably as a result of niche adaptation.
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spelling pubmed-32818302012-02-23 Hospital and Community Ampicillin-Resistant Enterococcus faecium Are Evolutionarily Closely Linked but Have Diversified through Niche Adaptation de Regt, Marieke J. A. van Schaik, Willem van Luit-Asbroek, Miranda Dekker, Huberta A. T. van Duijkeren, Engeline Koning, Catherina J. M. Bonten, Marc J. M. Willems, Rob J. L. PLoS One Research Article BACKGROUND: Ampicillin-resistant Enterococcus faecium (ARE) has emerged as a nosocomial pathogen. Here, we quantified ARE carriage in different community sources and determined genetic relatedness with hospital ARE. METHODS AND RESULTS: ARE was recovered from rectal swabs of 24 of 79 (30%) dogs, 11 of 85 (13%) cats and 0 of 42 horses and from 3 of 40 (8%) faecal samples of non-hospitalized humans receiving amoxicillin. Multi-locus Sequence Typing revealed 21 sequence types (STs), including 5 STs frequently associated with hospital-acquired infections. Genes previously found to be enriched in hospital ARE, such as IS16, orf903, orf905, orf907, were highly prevalent in community ARE (≥79%), while genes with a proposed role in pathogenesis, such as esp, hyl and ecbA, were found rarely (≤5%) in community isolates. Comparative genome analysis of 2 representative dog isolates revealed that the dog strain of ST192 was evolutionarily closely linked to two previously sequenced hospital ARE, but had, based on gene content, more genes in common with the other, evolutionarily more distantly related, dog strain (ST266). CONCLUSION: ARE were detected in dogs, cats and sporadically in healthy humans, with evolutionary linkage to hospital ARE. Yet, their accessory genome has diversified, probably as a result of niche adaptation. Public Library of Science 2012-02-17 /pmc/articles/PMC3281830/ /pubmed/22363425 http://dx.doi.org/10.1371/journal.pone.0030319 Text en de Regt et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
de Regt, Marieke J. A.
van Schaik, Willem
van Luit-Asbroek, Miranda
Dekker, Huberta A. T.
van Duijkeren, Engeline
Koning, Catherina J. M.
Bonten, Marc J. M.
Willems, Rob J. L.
Hospital and Community Ampicillin-Resistant Enterococcus faecium Are Evolutionarily Closely Linked but Have Diversified through Niche Adaptation
title Hospital and Community Ampicillin-Resistant Enterococcus faecium Are Evolutionarily Closely Linked but Have Diversified through Niche Adaptation
title_full Hospital and Community Ampicillin-Resistant Enterococcus faecium Are Evolutionarily Closely Linked but Have Diversified through Niche Adaptation
title_fullStr Hospital and Community Ampicillin-Resistant Enterococcus faecium Are Evolutionarily Closely Linked but Have Diversified through Niche Adaptation
title_full_unstemmed Hospital and Community Ampicillin-Resistant Enterococcus faecium Are Evolutionarily Closely Linked but Have Diversified through Niche Adaptation
title_short Hospital and Community Ampicillin-Resistant Enterococcus faecium Are Evolutionarily Closely Linked but Have Diversified through Niche Adaptation
title_sort hospital and community ampicillin-resistant enterococcus faecium are evolutionarily closely linked but have diversified through niche adaptation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281830/
https://www.ncbi.nlm.nih.gov/pubmed/22363425
http://dx.doi.org/10.1371/journal.pone.0030319
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