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Antagonistic Roles of SEPALLATA3, FT and FLC Genes as Targets of the Polycomb Group Gene CURLY LEAF

In Arabidopsis, mutations in the Pc-G gene CURLY LEAF (CLF) give early flowering plants with curled leaves. This phenotype is caused by mis-expression of the floral homeotic gene AGAMOUS (AG) in leaves, so that ag mutations largely suppress the clf phenotype. Here, we identify three mutations that s...

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Autores principales: Lopez-Vernaza, Manuel, Yang, Suxin, Müller, Ralf, Thorpe, Frazer, de Leau, Erica, Goodrich, Justin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281876/
https://www.ncbi.nlm.nih.gov/pubmed/22363474
http://dx.doi.org/10.1371/journal.pone.0030715
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author Lopez-Vernaza, Manuel
Yang, Suxin
Müller, Ralf
Thorpe, Frazer
de Leau, Erica
Goodrich, Justin
author_facet Lopez-Vernaza, Manuel
Yang, Suxin
Müller, Ralf
Thorpe, Frazer
de Leau, Erica
Goodrich, Justin
author_sort Lopez-Vernaza, Manuel
collection PubMed
description In Arabidopsis, mutations in the Pc-G gene CURLY LEAF (CLF) give early flowering plants with curled leaves. This phenotype is caused by mis-expression of the floral homeotic gene AGAMOUS (AG) in leaves, so that ag mutations largely suppress the clf phenotype. Here, we identify three mutations that suppress clf despite maintaining high AG expression. We show that the suppressors correspond to mutations in FPA and FT, two genes promoting flowering, and in SEPALLATA3 (SEP3) which encodes a co-factor for AG protein. The suppression of the clf phenotype is correlated with low SEP3 expression in all case and reveals that SEP3 has a role in promoting flowering in addition to its role in controlling floral organ identity. Genetic analysis of clf ft mutants indicates that CLF promotes flowering by reducing expression of FLC, a repressor of flowering. We conclude that SEP3 is the key target mediating the clf phenotype, and that the antagonistic effects of CLF target genes masks a role for CLF in promoting flowering.
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spelling pubmed-32818762012-02-23 Antagonistic Roles of SEPALLATA3, FT and FLC Genes as Targets of the Polycomb Group Gene CURLY LEAF Lopez-Vernaza, Manuel Yang, Suxin Müller, Ralf Thorpe, Frazer de Leau, Erica Goodrich, Justin PLoS One Research Article In Arabidopsis, mutations in the Pc-G gene CURLY LEAF (CLF) give early flowering plants with curled leaves. This phenotype is caused by mis-expression of the floral homeotic gene AGAMOUS (AG) in leaves, so that ag mutations largely suppress the clf phenotype. Here, we identify three mutations that suppress clf despite maintaining high AG expression. We show that the suppressors correspond to mutations in FPA and FT, two genes promoting flowering, and in SEPALLATA3 (SEP3) which encodes a co-factor for AG protein. The suppression of the clf phenotype is correlated with low SEP3 expression in all case and reveals that SEP3 has a role in promoting flowering in addition to its role in controlling floral organ identity. Genetic analysis of clf ft mutants indicates that CLF promotes flowering by reducing expression of FLC, a repressor of flowering. We conclude that SEP3 is the key target mediating the clf phenotype, and that the antagonistic effects of CLF target genes masks a role for CLF in promoting flowering. Public Library of Science 2012-02-17 /pmc/articles/PMC3281876/ /pubmed/22363474 http://dx.doi.org/10.1371/journal.pone.0030715 Text en Lopez-Vernaza et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lopez-Vernaza, Manuel
Yang, Suxin
Müller, Ralf
Thorpe, Frazer
de Leau, Erica
Goodrich, Justin
Antagonistic Roles of SEPALLATA3, FT and FLC Genes as Targets of the Polycomb Group Gene CURLY LEAF
title Antagonistic Roles of SEPALLATA3, FT and FLC Genes as Targets of the Polycomb Group Gene CURLY LEAF
title_full Antagonistic Roles of SEPALLATA3, FT and FLC Genes as Targets of the Polycomb Group Gene CURLY LEAF
title_fullStr Antagonistic Roles of SEPALLATA3, FT and FLC Genes as Targets of the Polycomb Group Gene CURLY LEAF
title_full_unstemmed Antagonistic Roles of SEPALLATA3, FT and FLC Genes as Targets of the Polycomb Group Gene CURLY LEAF
title_short Antagonistic Roles of SEPALLATA3, FT and FLC Genes as Targets of the Polycomb Group Gene CURLY LEAF
title_sort antagonistic roles of sepallata3, ft and flc genes as targets of the polycomb group gene curly leaf
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281876/
https://www.ncbi.nlm.nih.gov/pubmed/22363474
http://dx.doi.org/10.1371/journal.pone.0030715
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