Cargando…
The Neuroprotective Potential of Cyanidin-3-glucoside Fraction Extracted from Mulberry Following Oxygen-glucose Deprivation
In this study, cyanidin-3-glucoside (C3G) fraction extracted from the mulberry fruit (Morus alba L.) was investigated for its neuroprotective effects against oxygen-glucose deprivation (OGD) and glutamate-induced cell death in rat primary cortical neurons. Cell membrane damage and mitochondrial func...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Physiological Society and The Korean Society of Pharmacology
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282223/ https://www.ncbi.nlm.nih.gov/pubmed/22359473 http://dx.doi.org/10.4196/kjpp.2011.15.6.353 |
_version_ | 1782224049835868160 |
---|---|
author | Bhuiyan, Mohammad Iqbal Hossain Kim, Hyun-Bok Kim, Seong Yun Cho, Kyung-Ok |
author_facet | Bhuiyan, Mohammad Iqbal Hossain Kim, Hyun-Bok Kim, Seong Yun Cho, Kyung-Ok |
author_sort | Bhuiyan, Mohammad Iqbal Hossain |
collection | PubMed |
description | In this study, cyanidin-3-glucoside (C3G) fraction extracted from the mulberry fruit (Morus alba L.) was investigated for its neuroprotective effects against oxygen-glucose deprivation (OGD) and glutamate-induced cell death in rat primary cortical neurons. Cell membrane damage and mitochondrial function were assessed by LDH release and MTT reduction assays, respectively. A time-course study of OGD-induced cell death of primary cortical neurons at 7 days in vitro (DIV) indicated that neuronal death was OGD duration-dependent. It was also demonstrated that OGD for 3.5 h resulted in approximately 50% cell death, as determined by the LDH release assay. Treatments with mulberry C3G fraction prevented membrane damage and preserved the mitochondrial function of the primary cortical neurons exposed to OGD for 3.5 h in a concentration-dependent manner. Glutamate-induced cell death was more pronounced in DIV-9 and DIV-11 cells than that in DIV-7 neurons, and an application of 50µM glutamate was shown to induce approximately 40% cell death in DIV-9 neurons. Interestingly, treatment with mulberry C3G fraction did not provide a protective effect against glutamate-induced cell death in primary cortical neurons. On the other hand, treatment with mulberry C3G fraction maintained the mitochondrial membrane potential (MMP) in primary cortical neurons exposed to OGD as assessed by the intensity of rhodamine-123 fluorescence. These results therefore suggest that the neuroprotective effects of mulberry C3G fraction are mediated by the maintenance of the MMP and mitochondrial function but not by attenuating glutamate-induced excitotoxicity in rat primary cortical neurons. |
format | Online Article Text |
id | pubmed-3282223 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Korean Physiological Society and The Korean Society of Pharmacology |
record_format | MEDLINE/PubMed |
spelling | pubmed-32822232012-02-22 The Neuroprotective Potential of Cyanidin-3-glucoside Fraction Extracted from Mulberry Following Oxygen-glucose Deprivation Bhuiyan, Mohammad Iqbal Hossain Kim, Hyun-Bok Kim, Seong Yun Cho, Kyung-Ok Korean J Physiol Pharmacol Original Article In this study, cyanidin-3-glucoside (C3G) fraction extracted from the mulberry fruit (Morus alba L.) was investigated for its neuroprotective effects against oxygen-glucose deprivation (OGD) and glutamate-induced cell death in rat primary cortical neurons. Cell membrane damage and mitochondrial function were assessed by LDH release and MTT reduction assays, respectively. A time-course study of OGD-induced cell death of primary cortical neurons at 7 days in vitro (DIV) indicated that neuronal death was OGD duration-dependent. It was also demonstrated that OGD for 3.5 h resulted in approximately 50% cell death, as determined by the LDH release assay. Treatments with mulberry C3G fraction prevented membrane damage and preserved the mitochondrial function of the primary cortical neurons exposed to OGD for 3.5 h in a concentration-dependent manner. Glutamate-induced cell death was more pronounced in DIV-9 and DIV-11 cells than that in DIV-7 neurons, and an application of 50µM glutamate was shown to induce approximately 40% cell death in DIV-9 neurons. Interestingly, treatment with mulberry C3G fraction did not provide a protective effect against glutamate-induced cell death in primary cortical neurons. On the other hand, treatment with mulberry C3G fraction maintained the mitochondrial membrane potential (MMP) in primary cortical neurons exposed to OGD as assessed by the intensity of rhodamine-123 fluorescence. These results therefore suggest that the neuroprotective effects of mulberry C3G fraction are mediated by the maintenance of the MMP and mitochondrial function but not by attenuating glutamate-induced excitotoxicity in rat primary cortical neurons. The Korean Physiological Society and The Korean Society of Pharmacology 2011-12 2011-12-27 /pmc/articles/PMC3282223/ /pubmed/22359473 http://dx.doi.org/10.4196/kjpp.2011.15.6.353 Text en Copyright © 2011 The Korean Physiological Society and The Korean Society of Pharmacology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Bhuiyan, Mohammad Iqbal Hossain Kim, Hyun-Bok Kim, Seong Yun Cho, Kyung-Ok The Neuroprotective Potential of Cyanidin-3-glucoside Fraction Extracted from Mulberry Following Oxygen-glucose Deprivation |
title | The Neuroprotective Potential of Cyanidin-3-glucoside Fraction Extracted from Mulberry Following Oxygen-glucose Deprivation |
title_full | The Neuroprotective Potential of Cyanidin-3-glucoside Fraction Extracted from Mulberry Following Oxygen-glucose Deprivation |
title_fullStr | The Neuroprotective Potential of Cyanidin-3-glucoside Fraction Extracted from Mulberry Following Oxygen-glucose Deprivation |
title_full_unstemmed | The Neuroprotective Potential of Cyanidin-3-glucoside Fraction Extracted from Mulberry Following Oxygen-glucose Deprivation |
title_short | The Neuroprotective Potential of Cyanidin-3-glucoside Fraction Extracted from Mulberry Following Oxygen-glucose Deprivation |
title_sort | neuroprotective potential of cyanidin-3-glucoside fraction extracted from mulberry following oxygen-glucose deprivation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282223/ https://www.ncbi.nlm.nih.gov/pubmed/22359473 http://dx.doi.org/10.4196/kjpp.2011.15.6.353 |
work_keys_str_mv | AT bhuiyanmohammadiqbalhossain theneuroprotectivepotentialofcyanidin3glucosidefractionextractedfrommulberryfollowingoxygenglucosedeprivation AT kimhyunbok theneuroprotectivepotentialofcyanidin3glucosidefractionextractedfrommulberryfollowingoxygenglucosedeprivation AT kimseongyun theneuroprotectivepotentialofcyanidin3glucosidefractionextractedfrommulberryfollowingoxygenglucosedeprivation AT chokyungok theneuroprotectivepotentialofcyanidin3glucosidefractionextractedfrommulberryfollowingoxygenglucosedeprivation AT bhuiyanmohammadiqbalhossain neuroprotectivepotentialofcyanidin3glucosidefractionextractedfrommulberryfollowingoxygenglucosedeprivation AT kimhyunbok neuroprotectivepotentialofcyanidin3glucosidefractionextractedfrommulberryfollowingoxygenglucosedeprivation AT kimseongyun neuroprotectivepotentialofcyanidin3glucosidefractionextractedfrommulberryfollowingoxygenglucosedeprivation AT chokyungok neuroprotectivepotentialofcyanidin3glucosidefractionextractedfrommulberryfollowingoxygenglucosedeprivation |