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Role of Regulators of G-Protein Signaling 4 in Ca(2+) Signaling in Mouse Pancreatic Acinar Cells
Regulators of G-protein signaling (RGS) proteins are regulators of Ca(2+) signaling that accelerate the GTPase activity of the G-protein α-subunit. RGS1, RGS2, RGS4, and RGS16 are expressed in the pancreas, and RGS2 regulates G-protein coupled receptor (GPCR)-induced Ca(2+) oscillations. However, th...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Physiological Society and The Korean Society of Pharmacology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282226/ https://www.ncbi.nlm.nih.gov/pubmed/22359476 http://dx.doi.org/10.4196/kjpp.2011.15.6.383 |
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author | Park, Soonhong Lee, Syng-Ill Shin, Dong Min |
author_facet | Park, Soonhong Lee, Syng-Ill Shin, Dong Min |
author_sort | Park, Soonhong |
collection | PubMed |
description | Regulators of G-protein signaling (RGS) proteins are regulators of Ca(2+) signaling that accelerate the GTPase activity of the G-protein α-subunit. RGS1, RGS2, RGS4, and RGS16 are expressed in the pancreas, and RGS2 regulates G-protein coupled receptor (GPCR)-induced Ca(2+) oscillations. However, the role of RGS4 in Ca(2+) signaling in pancreatic acinar cells is unknown. In this study, we investigated the mechanism of GPCR-induced Ca(2+) signaling in pancreatic acinar cells derived from RGS4(-/-) mice. RGS4(-/-) acinar cells showed an enhanced stimulus intensity response to a muscarinic receptor agonist in pancreatic acinar cells. Moreover, deletion of RGS4 increased the frequency of Ca(2+) oscillations. RGS4(-/-) cells also showed increased expression of sarco/endoplasmic reticulum Ca(2+) ATPase type 2. However, there were no significant alterations, such as Ca(2+) signaling in treated high dose of agonist and its related amylase secretion activity, in acinar cells from RGS4(-/-) mice. These results indicate that RGS4 protein regulates Ca(2+) signaling in mouse pancreatic acinar cells. |
format | Online Article Text |
id | pubmed-3282226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Korean Physiological Society and The Korean Society of Pharmacology |
record_format | MEDLINE/PubMed |
spelling | pubmed-32822262012-02-22 Role of Regulators of G-Protein Signaling 4 in Ca(2+) Signaling in Mouse Pancreatic Acinar Cells Park, Soonhong Lee, Syng-Ill Shin, Dong Min Korean J Physiol Pharmacol Original Article Regulators of G-protein signaling (RGS) proteins are regulators of Ca(2+) signaling that accelerate the GTPase activity of the G-protein α-subunit. RGS1, RGS2, RGS4, and RGS16 are expressed in the pancreas, and RGS2 regulates G-protein coupled receptor (GPCR)-induced Ca(2+) oscillations. However, the role of RGS4 in Ca(2+) signaling in pancreatic acinar cells is unknown. In this study, we investigated the mechanism of GPCR-induced Ca(2+) signaling in pancreatic acinar cells derived from RGS4(-/-) mice. RGS4(-/-) acinar cells showed an enhanced stimulus intensity response to a muscarinic receptor agonist in pancreatic acinar cells. Moreover, deletion of RGS4 increased the frequency of Ca(2+) oscillations. RGS4(-/-) cells also showed increased expression of sarco/endoplasmic reticulum Ca(2+) ATPase type 2. However, there were no significant alterations, such as Ca(2+) signaling in treated high dose of agonist and its related amylase secretion activity, in acinar cells from RGS4(-/-) mice. These results indicate that RGS4 protein regulates Ca(2+) signaling in mouse pancreatic acinar cells. The Korean Physiological Society and The Korean Society of Pharmacology 2011-12 2011-12-27 /pmc/articles/PMC3282226/ /pubmed/22359476 http://dx.doi.org/10.4196/kjpp.2011.15.6.383 Text en Copyright © 2011 The Korean Physiological Society and The Korean Society of Pharmacology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Park, Soonhong Lee, Syng-Ill Shin, Dong Min Role of Regulators of G-Protein Signaling 4 in Ca(2+) Signaling in Mouse Pancreatic Acinar Cells |
title | Role of Regulators of G-Protein Signaling 4 in Ca(2+) Signaling in Mouse Pancreatic Acinar Cells |
title_full | Role of Regulators of G-Protein Signaling 4 in Ca(2+) Signaling in Mouse Pancreatic Acinar Cells |
title_fullStr | Role of Regulators of G-Protein Signaling 4 in Ca(2+) Signaling in Mouse Pancreatic Acinar Cells |
title_full_unstemmed | Role of Regulators of G-Protein Signaling 4 in Ca(2+) Signaling in Mouse Pancreatic Acinar Cells |
title_short | Role of Regulators of G-Protein Signaling 4 in Ca(2+) Signaling in Mouse Pancreatic Acinar Cells |
title_sort | role of regulators of g-protein signaling 4 in ca(2+) signaling in mouse pancreatic acinar cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282226/ https://www.ncbi.nlm.nih.gov/pubmed/22359476 http://dx.doi.org/10.4196/kjpp.2011.15.6.383 |
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