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The protection role of heat shock protein 70 (HSP-70) in the testes of cadmium-exposed rats

Cadmium (Cd) is an environmental carcinogenic pollutant known to inactivate several proteins involved in DNA repair systems while at the same time creating an oxidative stress that can result in additional DNA lesions. The testis and the lung are the target organs for cadmium carcinogenesis. Increas...

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Autores principales: Selim, Manar E, Rashed, El Hamidi A, Aleisa, Nadia A, Daghestani, Maha H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biomedical Informatics 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282277/
https://www.ncbi.nlm.nih.gov/pubmed/22359436
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author Selim, Manar E
Rashed, El Hamidi A
Aleisa, Nadia A
Daghestani, Maha H
author_facet Selim, Manar E
Rashed, El Hamidi A
Aleisa, Nadia A
Daghestani, Maha H
author_sort Selim, Manar E
collection PubMed
description Cadmium (Cd) is an environmental carcinogenic pollutant known to inactivate several proteins involved in DNA repair systems while at the same time creating an oxidative stress that can result in additional DNA lesions. The testis and the lung are the target organs for cadmium carcinogenesis. Increased production of oxidants in vivo can cause damage to intracellular macromolecules such as DNA, proteins and lipids, which in turn lead to oxidative injury. So, this investigation aimed to evaluate the protective role of L-Carnitine through up regulation of HSPs against DNA damage induced by cadmium chloride. The current study was carried out on forty adult male rats, each with average weight 220-250g., were divided into 4 equal groups. 1(st) group was received saline solution (0.5 ml/100 g body weight) and kept as control. 2(nd) group was received 500mg / kg body weight L-Carnitine intraperitoneally (IP). 3(rd) group was administered 1.2 mg cadmium chloride IP. 4(th) group was received both cadmium chloride and L-Carnitine simultaneously. The comet assay parameters showed significantly increased HSP70 and DNA damage in testis cells after 10 and 56 days in the third group. Meanwhile, HSP70 showed significantly decreased levels after 10 days and 56 days in the fourth group after L-Carnitine treatment simultaneously with cadmium chloride. The results of the present study demonstrate a damaging effect of cadmium chloride on DNA of the testis cells (with low stress response). This damaging effect increases the synthesis of HSP70 that upregulated by L-Carnitine treatment and showed ameliorative effect of the cells for recovery.
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spelling pubmed-32822772012-02-22 The protection role of heat shock protein 70 (HSP-70) in the testes of cadmium-exposed rats Selim, Manar E Rashed, El Hamidi A Aleisa, Nadia A Daghestani, Maha H Bioinformation Current Trends Cadmium (Cd) is an environmental carcinogenic pollutant known to inactivate several proteins involved in DNA repair systems while at the same time creating an oxidative stress that can result in additional DNA lesions. The testis and the lung are the target organs for cadmium carcinogenesis. Increased production of oxidants in vivo can cause damage to intracellular macromolecules such as DNA, proteins and lipids, which in turn lead to oxidative injury. So, this investigation aimed to evaluate the protective role of L-Carnitine through up regulation of HSPs against DNA damage induced by cadmium chloride. The current study was carried out on forty adult male rats, each with average weight 220-250g., were divided into 4 equal groups. 1(st) group was received saline solution (0.5 ml/100 g body weight) and kept as control. 2(nd) group was received 500mg / kg body weight L-Carnitine intraperitoneally (IP). 3(rd) group was administered 1.2 mg cadmium chloride IP. 4(th) group was received both cadmium chloride and L-Carnitine simultaneously. The comet assay parameters showed significantly increased HSP70 and DNA damage in testis cells after 10 and 56 days in the third group. Meanwhile, HSP70 showed significantly decreased levels after 10 days and 56 days in the fourth group after L-Carnitine treatment simultaneously with cadmium chloride. The results of the present study demonstrate a damaging effect of cadmium chloride on DNA of the testis cells (with low stress response). This damaging effect increases the synthesis of HSP70 that upregulated by L-Carnitine treatment and showed ameliorative effect of the cells for recovery. Biomedical Informatics 2012-01-06 /pmc/articles/PMC3282277/ /pubmed/22359436 Text en © 2012 Biomedical Informatics This is an open-access article, which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original author and source are credited.
spellingShingle Current Trends
Selim, Manar E
Rashed, El Hamidi A
Aleisa, Nadia A
Daghestani, Maha H
The protection role of heat shock protein 70 (HSP-70) in the testes of cadmium-exposed rats
title The protection role of heat shock protein 70 (HSP-70) in the testes of cadmium-exposed rats
title_full The protection role of heat shock protein 70 (HSP-70) in the testes of cadmium-exposed rats
title_fullStr The protection role of heat shock protein 70 (HSP-70) in the testes of cadmium-exposed rats
title_full_unstemmed The protection role of heat shock protein 70 (HSP-70) in the testes of cadmium-exposed rats
title_short The protection role of heat shock protein 70 (HSP-70) in the testes of cadmium-exposed rats
title_sort protection role of heat shock protein 70 (hsp-70) in the testes of cadmium-exposed rats
topic Current Trends
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282277/
https://www.ncbi.nlm.nih.gov/pubmed/22359436
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