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The significance of Y chromosome microdeletion analysis in subfertile men with clinical variocele

INTRODUCTION: The aim of study is determining the cost-effectiveness of detection analysis in the presence of exceptional patients who have mild semen disorders, and beware of unnecessary varicocele repairs; and to ascertain whether patients with clinical varicocele should undergo Y chromosome (Yq)...

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Autores principales: Sagnak, Levent, Ersoy, Hamit, Ozok, Ugur, Eraslan, Asir, Yararbas, Kanay, Goktug, Goksel, Tukun, Ajlan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282516/
https://www.ncbi.nlm.nih.gov/pubmed/22371775
http://dx.doi.org/10.5114/aoms.2010.14259
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author Sagnak, Levent
Ersoy, Hamit
Ozok, Ugur
Eraslan, Asir
Yararbas, Kanay
Goktug, Goksel
Tukun, Ajlan
author_facet Sagnak, Levent
Ersoy, Hamit
Ozok, Ugur
Eraslan, Asir
Yararbas, Kanay
Goktug, Goksel
Tukun, Ajlan
author_sort Sagnak, Levent
collection PubMed
description INTRODUCTION: The aim of study is determining the cost-effectiveness of detection analysis in the presence of exceptional patients who have mild semen disorders, and beware of unnecessary varicocele repairs; and to ascertain whether patients with clinical varicocele should undergo Y chromosome (Yq) microdeletion analysis as a routine procedure. MATERIAL AND METHODS: Varicocele with reflux was diagnosed in 51 male patients with subfertility symptoms upon physical examination (PE), confirmed by scrotal colour-Doppler ultrasound (CDU). After cytogenetic examination, Yq microdeletion analysis was performed on the peripheral blood samples using Promega Y Chromosome Deletion Detection System Version 2. Varicocele repair was performed under general anaesthesia with optical magnification (3-fold) through a subinguinal approach. RESULTS: The mean age of the patients was 27.9. Values of semen concentration ranged from 0 to 72 million/ml, motility from 0 to 65% (A + B) and Kruger from 0% to 18%. The PE revealed normal size and consistency in the bilateral testicles. All patients were cytogenetically normal. However, Yq microdeletion was detected in 2 patients, 1 with mild oligoteratozoospermia and partial AZFb deletion (sY121) and the second patient with severe oligozoospermia and partial AZFc deletion (sY254 and sY255), and they were not subjected to varicocelectomy. CONCLUSIONS: The routine performance of pre-operative Yq microdeletion analysis in patients with clinical varicocele does not seem to be cost-effective but the omission of patients with mild oligozoospermia would have subjected them to an unnecessary varicocelectomy and/or further ICSI applications and also would have caused the failure of referral for genetic counselling.
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spelling pubmed-32825162012-02-27 The significance of Y chromosome microdeletion analysis in subfertile men with clinical variocele Sagnak, Levent Ersoy, Hamit Ozok, Ugur Eraslan, Asir Yararbas, Kanay Goktug, Goksel Tukun, Ajlan Arch Med Sci Clinical Research INTRODUCTION: The aim of study is determining the cost-effectiveness of detection analysis in the presence of exceptional patients who have mild semen disorders, and beware of unnecessary varicocele repairs; and to ascertain whether patients with clinical varicocele should undergo Y chromosome (Yq) microdeletion analysis as a routine procedure. MATERIAL AND METHODS: Varicocele with reflux was diagnosed in 51 male patients with subfertility symptoms upon physical examination (PE), confirmed by scrotal colour-Doppler ultrasound (CDU). After cytogenetic examination, Yq microdeletion analysis was performed on the peripheral blood samples using Promega Y Chromosome Deletion Detection System Version 2. Varicocele repair was performed under general anaesthesia with optical magnification (3-fold) through a subinguinal approach. RESULTS: The mean age of the patients was 27.9. Values of semen concentration ranged from 0 to 72 million/ml, motility from 0 to 65% (A + B) and Kruger from 0% to 18%. The PE revealed normal size and consistency in the bilateral testicles. All patients were cytogenetically normal. However, Yq microdeletion was detected in 2 patients, 1 with mild oligoteratozoospermia and partial AZFb deletion (sY121) and the second patient with severe oligozoospermia and partial AZFc deletion (sY254 and sY255), and they were not subjected to varicocelectomy. CONCLUSIONS: The routine performance of pre-operative Yq microdeletion analysis in patients with clinical varicocele does not seem to be cost-effective but the omission of patients with mild oligozoospermia would have subjected them to an unnecessary varicocelectomy and/or further ICSI applications and also would have caused the failure of referral for genetic counselling. Termedia Publishing House 2010-06-30 2010-06-30 /pmc/articles/PMC3282516/ /pubmed/22371775 http://dx.doi.org/10.5114/aoms.2010.14259 Text en Copyright © 2010 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Research
Sagnak, Levent
Ersoy, Hamit
Ozok, Ugur
Eraslan, Asir
Yararbas, Kanay
Goktug, Goksel
Tukun, Ajlan
The significance of Y chromosome microdeletion analysis in subfertile men with clinical variocele
title The significance of Y chromosome microdeletion analysis in subfertile men with clinical variocele
title_full The significance of Y chromosome microdeletion analysis in subfertile men with clinical variocele
title_fullStr The significance of Y chromosome microdeletion analysis in subfertile men with clinical variocele
title_full_unstemmed The significance of Y chromosome microdeletion analysis in subfertile men with clinical variocele
title_short The significance of Y chromosome microdeletion analysis in subfertile men with clinical variocele
title_sort significance of y chromosome microdeletion analysis in subfertile men with clinical variocele
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282516/
https://www.ncbi.nlm.nih.gov/pubmed/22371775
http://dx.doi.org/10.5114/aoms.2010.14259
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