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Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound
PURPOSE: The purpose of this study was to evaluate the permeability of the blood–brain barrier after sonication by pulsed high-intensity focused ultrasound and to determine if such an approach increases the tumor:ipsilateral brain permeability ratio. MATERIALS AND METHODS: F98 glioma-bearing Fischer...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282611/ https://www.ncbi.nlm.nih.gov/pubmed/22359451 http://dx.doi.org/10.2147/IJN.S28503 |
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author | Yang, Feng-Yi Wang, Hsin-Ell Lin, Guan-Liang Lin, Hui-Hsien Wong, Tai-Tong |
author_facet | Yang, Feng-Yi Wang, Hsin-Ell Lin, Guan-Liang Lin, Hui-Hsien Wong, Tai-Tong |
author_sort | Yang, Feng-Yi |
collection | PubMed |
description | PURPOSE: The purpose of this study was to evaluate the permeability of the blood–brain barrier after sonication by pulsed high-intensity focused ultrasound and to determine if such an approach increases the tumor:ipsilateral brain permeability ratio. MATERIALS AND METHODS: F98 glioma-bearing Fischer 344 rats were injected intravenously with Evans blue with or without blood–tumor barrier disruption induced by transcranial pulsed high-intensity focused ultrasound. Sonication was applied at a frequency of 1 MHz with a 5% duty cycle and a repetition frequency of 1 Hz. The permeability of the blood–brain barrier was assessed by the extravasation of Evans blue. Contrast-enhanced magnetic resonance images were used to monitor the gadolinium deposition path associated with transcranial pulsed high-intensity focused ultrasound, and the influencing size and location was also investigated. In addition, whole brain histological analysis was performed. The results were compared by two-tailed unpaired t-test. RESULTS: The accumulation of Evans blue in brains and the tumor:ipsilateral brain permeability ratio of Evans blue were significantly increased after pulsed high-intensity focused ultrasound exposure. Evans blue injection followed by sonication showed an increase in the tumor:ipsilateral brain ratio of the target tumors (9.14:1) of about 2.23-fold compared with the control tumors (x4.09) on day 6 after tumor implantation. Magnetic resonance images showed that pulsed high-intensity focused ultrasound locally enhances the permeability of the blood–tumor barrier in the glioma-bearing rats. CONCLUSION: This method could allow enhanced synergistic effects with respect to other brain tumor treatment regimens. |
format | Online Article Text |
id | pubmed-3282611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-32826112012-02-22 Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound Yang, Feng-Yi Wang, Hsin-Ell Lin, Guan-Liang Lin, Hui-Hsien Wong, Tai-Tong Int J Nanomedicine Original Research PURPOSE: The purpose of this study was to evaluate the permeability of the blood–brain barrier after sonication by pulsed high-intensity focused ultrasound and to determine if such an approach increases the tumor:ipsilateral brain permeability ratio. MATERIALS AND METHODS: F98 glioma-bearing Fischer 344 rats were injected intravenously with Evans blue with or without blood–tumor barrier disruption induced by transcranial pulsed high-intensity focused ultrasound. Sonication was applied at a frequency of 1 MHz with a 5% duty cycle and a repetition frequency of 1 Hz. The permeability of the blood–brain barrier was assessed by the extravasation of Evans blue. Contrast-enhanced magnetic resonance images were used to monitor the gadolinium deposition path associated with transcranial pulsed high-intensity focused ultrasound, and the influencing size and location was also investigated. In addition, whole brain histological analysis was performed. The results were compared by two-tailed unpaired t-test. RESULTS: The accumulation of Evans blue in brains and the tumor:ipsilateral brain permeability ratio of Evans blue were significantly increased after pulsed high-intensity focused ultrasound exposure. Evans blue injection followed by sonication showed an increase in the tumor:ipsilateral brain ratio of the target tumors (9.14:1) of about 2.23-fold compared with the control tumors (x4.09) on day 6 after tumor implantation. Magnetic resonance images showed that pulsed high-intensity focused ultrasound locally enhances the permeability of the blood–tumor barrier in the glioma-bearing rats. CONCLUSION: This method could allow enhanced synergistic effects with respect to other brain tumor treatment regimens. Dove Medical Press 2012 2012-02-13 /pmc/articles/PMC3282611/ /pubmed/22359451 http://dx.doi.org/10.2147/IJN.S28503 Text en © 2012 Yang et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Yang, Feng-Yi Wang, Hsin-Ell Lin, Guan-Liang Lin, Hui-Hsien Wong, Tai-Tong Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
title | Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
title_full | Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
title_fullStr | Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
title_full_unstemmed | Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
title_short | Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
title_sort | evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282611/ https://www.ncbi.nlm.nih.gov/pubmed/22359451 http://dx.doi.org/10.2147/IJN.S28503 |
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