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Putative Chemosensory Receptors of the Codling Moth, Cydia pomonella, Identified by Antennal Transcriptome Analysis

The codling moth, Cydia pomonella, is an important fruit pest worldwide. As nocturnal animals, adults depend to a large extent on olfactory cues for detection of food and mates, and, for females, oviposition sites. In insects, odor detection is mediated by odorant receptors (ORs) and ionotropic rece...

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Autores principales: Bengtsson, Jonas M., Trona, Federica, Montagné, Nicolas, Anfora, Gianfranco, Ignell, Rickard, Witzgall, Peter, Jacquin-Joly, Emmanuelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282773/
https://www.ncbi.nlm.nih.gov/pubmed/22363688
http://dx.doi.org/10.1371/journal.pone.0031620
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author Bengtsson, Jonas M.
Trona, Federica
Montagné, Nicolas
Anfora, Gianfranco
Ignell, Rickard
Witzgall, Peter
Jacquin-Joly, Emmanuelle
author_facet Bengtsson, Jonas M.
Trona, Federica
Montagné, Nicolas
Anfora, Gianfranco
Ignell, Rickard
Witzgall, Peter
Jacquin-Joly, Emmanuelle
author_sort Bengtsson, Jonas M.
collection PubMed
description The codling moth, Cydia pomonella, is an important fruit pest worldwide. As nocturnal animals, adults depend to a large extent on olfactory cues for detection of food and mates, and, for females, oviposition sites. In insects, odor detection is mediated by odorant receptors (ORs) and ionotropic receptors (IRs), which ensure the specificity of the olfactory sensory neuron responses. In this study, our aim was to identify chemosensory receptors in the codling moth as a means to uncover new targets for behavioral interference. Using next-generation sequencing techniques, we identified a total of 43 candidate ORs, one gustatory receptor and 15 IRs in the antennal transcriptome. Through Blast and sequence similarity analyses we annotated the insect obligatory co-receptor ORco, five genes clustering in a conserved clade containing sex pheromone receptors, one homolog of the Bombyx mori female-enriched receptor BmorOR30 (but no homologs of the other B. mori female-enriched receptors) and one gene clustering in the sugar receptor family. Among the candidate IRs, we identified homologs of the two highly conserved co-receptors IR8a and IR25a, and one homolog of an IR involved in phenylethyl amine detection in Drosophila. Our results open for functional characterization of the chemosensory receptors of C. pomonella, with potential for new or refined applications of semiochemicals for control of this pest insect.
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spelling pubmed-32827732012-02-23 Putative Chemosensory Receptors of the Codling Moth, Cydia pomonella, Identified by Antennal Transcriptome Analysis Bengtsson, Jonas M. Trona, Federica Montagné, Nicolas Anfora, Gianfranco Ignell, Rickard Witzgall, Peter Jacquin-Joly, Emmanuelle PLoS One Research Article The codling moth, Cydia pomonella, is an important fruit pest worldwide. As nocturnal animals, adults depend to a large extent on olfactory cues for detection of food and mates, and, for females, oviposition sites. In insects, odor detection is mediated by odorant receptors (ORs) and ionotropic receptors (IRs), which ensure the specificity of the olfactory sensory neuron responses. In this study, our aim was to identify chemosensory receptors in the codling moth as a means to uncover new targets for behavioral interference. Using next-generation sequencing techniques, we identified a total of 43 candidate ORs, one gustatory receptor and 15 IRs in the antennal transcriptome. Through Blast and sequence similarity analyses we annotated the insect obligatory co-receptor ORco, five genes clustering in a conserved clade containing sex pheromone receptors, one homolog of the Bombyx mori female-enriched receptor BmorOR30 (but no homologs of the other B. mori female-enriched receptors) and one gene clustering in the sugar receptor family. Among the candidate IRs, we identified homologs of the two highly conserved co-receptors IR8a and IR25a, and one homolog of an IR involved in phenylethyl amine detection in Drosophila. Our results open for functional characterization of the chemosensory receptors of C. pomonella, with potential for new or refined applications of semiochemicals for control of this pest insect. Public Library of Science 2012-02-20 /pmc/articles/PMC3282773/ /pubmed/22363688 http://dx.doi.org/10.1371/journal.pone.0031620 Text en Bengtsson et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bengtsson, Jonas M.
Trona, Federica
Montagné, Nicolas
Anfora, Gianfranco
Ignell, Rickard
Witzgall, Peter
Jacquin-Joly, Emmanuelle
Putative Chemosensory Receptors of the Codling Moth, Cydia pomonella, Identified by Antennal Transcriptome Analysis
title Putative Chemosensory Receptors of the Codling Moth, Cydia pomonella, Identified by Antennal Transcriptome Analysis
title_full Putative Chemosensory Receptors of the Codling Moth, Cydia pomonella, Identified by Antennal Transcriptome Analysis
title_fullStr Putative Chemosensory Receptors of the Codling Moth, Cydia pomonella, Identified by Antennal Transcriptome Analysis
title_full_unstemmed Putative Chemosensory Receptors of the Codling Moth, Cydia pomonella, Identified by Antennal Transcriptome Analysis
title_short Putative Chemosensory Receptors of the Codling Moth, Cydia pomonella, Identified by Antennal Transcriptome Analysis
title_sort putative chemosensory receptors of the codling moth, cydia pomonella, identified by antennal transcriptome analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282773/
https://www.ncbi.nlm.nih.gov/pubmed/22363688
http://dx.doi.org/10.1371/journal.pone.0031620
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