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Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin
The Enteritidis and Dublin serovars of Salmonella enterica are closely related, yet they differ significantly in pathogenicity and epidemiology. S. Enteritidis is a broad host range serovar that commonly causes gastroenteritis and infrequently causes invasive disease in humans. S. Dublin mainly colo...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bentham Open
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282883/ https://www.ncbi.nlm.nih.gov/pubmed/22371816 http://dx.doi.org/10.2174/1874285801206010005 |
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author | Betancor, Laura Yim, Lucía Martínez, Arací Fookes, Maria Sasias, Sebastian Schelotto, Felipe Thomson, Nicholas Maskell, Duncan Chabalgoity, José A |
author_facet | Betancor, Laura Yim, Lucía Martínez, Arací Fookes, Maria Sasias, Sebastian Schelotto, Felipe Thomson, Nicholas Maskell, Duncan Chabalgoity, José A |
author_sort | Betancor, Laura |
collection | PubMed |
description | The Enteritidis and Dublin serovars of Salmonella enterica are closely related, yet they differ significantly in pathogenicity and epidemiology. S. Enteritidis is a broad host range serovar that commonly causes gastroenteritis and infrequently causes invasive disease in humans. S. Dublin mainly colonizes cattle but upon infecting humans often results in invasive disease.To gain a broader view of the extent of these differences we conducted microarray-based comparative genomics between several field isolates from each serovar. Genome degradation has been correlated with host adaptation in Salmonella, thus we also compared at whole genome scale the available genomic sequences of them to evaluate pseudogene composition within each serovar. Microarray analysis revealed 3771 CDS shared by both serovars while 33 were only present in Enteritidis and 87 were exclusive to Dublin. Pseudogene evaluation showed 177 inactive CDS in S. Dublin which correspond to active genes in S. Enteritidis, nine of which are also inactive in the host adapted S. Gallinarum and S. Choleraesuis serovars. Sequencing of these 9 CDS in several S. Dublin clinical isolates revealed that they are pseudogenes in all of them, indicating that this feature is not peculiar to the sequenced strain. Among these CDS, shdA (Peyer´s patch colonization factor) and mglA (galactoside transport ATP binding protein), appear also to be inactive in the human adapted S. Typhi and S. Paratyphi A, suggesting that functionality of these genes may be relevant for the capacity of certain Salmonella serovars to infect a broad range of hosts. |
format | Online Article Text |
id | pubmed-3282883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Bentham Open |
record_format | MEDLINE/PubMed |
spelling | pubmed-32828832012-02-27 Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin Betancor, Laura Yim, Lucía Martínez, Arací Fookes, Maria Sasias, Sebastian Schelotto, Felipe Thomson, Nicholas Maskell, Duncan Chabalgoity, José A Open Microbiol J Article The Enteritidis and Dublin serovars of Salmonella enterica are closely related, yet they differ significantly in pathogenicity and epidemiology. S. Enteritidis is a broad host range serovar that commonly causes gastroenteritis and infrequently causes invasive disease in humans. S. Dublin mainly colonizes cattle but upon infecting humans often results in invasive disease.To gain a broader view of the extent of these differences we conducted microarray-based comparative genomics between several field isolates from each serovar. Genome degradation has been correlated with host adaptation in Salmonella, thus we also compared at whole genome scale the available genomic sequences of them to evaluate pseudogene composition within each serovar. Microarray analysis revealed 3771 CDS shared by both serovars while 33 were only present in Enteritidis and 87 were exclusive to Dublin. Pseudogene evaluation showed 177 inactive CDS in S. Dublin which correspond to active genes in S. Enteritidis, nine of which are also inactive in the host adapted S. Gallinarum and S. Choleraesuis serovars. Sequencing of these 9 CDS in several S. Dublin clinical isolates revealed that they are pseudogenes in all of them, indicating that this feature is not peculiar to the sequenced strain. Among these CDS, shdA (Peyer´s patch colonization factor) and mglA (galactoside transport ATP binding protein), appear also to be inactive in the human adapted S. Typhi and S. Paratyphi A, suggesting that functionality of these genes may be relevant for the capacity of certain Salmonella serovars to infect a broad range of hosts. Bentham Open 2012-02-10 /pmc/articles/PMC3282883/ /pubmed/22371816 http://dx.doi.org/10.2174/1874285801206010005 Text en © Betancor et al.; Licensee Bentham Open. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited. |
spellingShingle | Article Betancor, Laura Yim, Lucía Martínez, Arací Fookes, Maria Sasias, Sebastian Schelotto, Felipe Thomson, Nicholas Maskell, Duncan Chabalgoity, José A Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin |
title | Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin |
title_full | Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin |
title_fullStr | Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin |
title_full_unstemmed | Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin |
title_short | Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin |
title_sort | genomic comparison of the closely related salmonella enterica serovars enteritidis and dublin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282883/ https://www.ncbi.nlm.nih.gov/pubmed/22371816 http://dx.doi.org/10.2174/1874285801206010005 |
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