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De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids

Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome con...

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Autores principales: Li, Haiyan, Dong, Yuanyuan, Yang, Jing, Liu, Xiuming, Wang, Yanfang, Yao, Na, Guan, Lili, Wang, Nan, Wu, Jinyu, Li, Xiaokun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283594/
https://www.ncbi.nlm.nih.gov/pubmed/22363528
http://dx.doi.org/10.1371/journal.pone.0030987
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author Li, Haiyan
Dong, Yuanyuan
Yang, Jing
Liu, Xiuming
Wang, Yanfang
Yao, Na
Guan, Lili
Wang, Nan
Wu, Jinyu
Li, Xiaokun
author_facet Li, Haiyan
Dong, Yuanyuan
Yang, Jing
Liu, Xiuming
Wang, Yanfang
Yao, Na
Guan, Lili
Wang, Nan
Wu, Jinyu
Li, Xiaokun
author_sort Li, Haiyan
collection PubMed
description Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome consisting of 153,769 unigenes. We annotated 82,916 of the unigenes with gene annotation and assigned functional terms and specific pathways to a subset of them. Metabolic pathway analysis revealed that 23 unigenes were predicted to be responsible for the biosynthesis of flavonoids and 8 were characterized as seed-specific oleosins. In addition, a large number of differentially expressed unigenes, for example, those annotated as participating in anthocyanin and chalcone synthesis, were predicted to be involved in flavonoid biosynthesis pathways. In conclusion, the de novo transcriptome investigation of the unique transcripts provided candidate gene resources for studying oleosin-coding genes and for investigating genes related to flavonoid biosynthesis and metabolism in safflower.
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spelling pubmed-32835942012-02-23 De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids Li, Haiyan Dong, Yuanyuan Yang, Jing Liu, Xiuming Wang, Yanfang Yao, Na Guan, Lili Wang, Nan Wu, Jinyu Li, Xiaokun PLoS One Research Article Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome consisting of 153,769 unigenes. We annotated 82,916 of the unigenes with gene annotation and assigned functional terms and specific pathways to a subset of them. Metabolic pathway analysis revealed that 23 unigenes were predicted to be responsible for the biosynthesis of flavonoids and 8 were characterized as seed-specific oleosins. In addition, a large number of differentially expressed unigenes, for example, those annotated as participating in anthocyanin and chalcone synthesis, were predicted to be involved in flavonoid biosynthesis pathways. In conclusion, the de novo transcriptome investigation of the unique transcripts provided candidate gene resources for studying oleosin-coding genes and for investigating genes related to flavonoid biosynthesis and metabolism in safflower. Public Library of Science 2012-02-21 /pmc/articles/PMC3283594/ /pubmed/22363528 http://dx.doi.org/10.1371/journal.pone.0030987 Text en Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Haiyan
Dong, Yuanyuan
Yang, Jing
Liu, Xiuming
Wang, Yanfang
Yao, Na
Guan, Lili
Wang, Nan
Wu, Jinyu
Li, Xiaokun
De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids
title De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids
title_full De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids
title_fullStr De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids
title_full_unstemmed De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids
title_short De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids
title_sort de novo transcriptome of safflower and the identification of putative genes for oleosin and the biosynthesis of flavonoids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283594/
https://www.ncbi.nlm.nih.gov/pubmed/22363528
http://dx.doi.org/10.1371/journal.pone.0030987
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