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De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids
Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome con...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283594/ https://www.ncbi.nlm.nih.gov/pubmed/22363528 http://dx.doi.org/10.1371/journal.pone.0030987 |
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author | Li, Haiyan Dong, Yuanyuan Yang, Jing Liu, Xiuming Wang, Yanfang Yao, Na Guan, Lili Wang, Nan Wu, Jinyu Li, Xiaokun |
author_facet | Li, Haiyan Dong, Yuanyuan Yang, Jing Liu, Xiuming Wang, Yanfang Yao, Na Guan, Lili Wang, Nan Wu, Jinyu Li, Xiaokun |
author_sort | Li, Haiyan |
collection | PubMed |
description | Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome consisting of 153,769 unigenes. We annotated 82,916 of the unigenes with gene annotation and assigned functional terms and specific pathways to a subset of them. Metabolic pathway analysis revealed that 23 unigenes were predicted to be responsible for the biosynthesis of flavonoids and 8 were characterized as seed-specific oleosins. In addition, a large number of differentially expressed unigenes, for example, those annotated as participating in anthocyanin and chalcone synthesis, were predicted to be involved in flavonoid biosynthesis pathways. In conclusion, the de novo transcriptome investigation of the unique transcripts provided candidate gene resources for studying oleosin-coding genes and for investigating genes related to flavonoid biosynthesis and metabolism in safflower. |
format | Online Article Text |
id | pubmed-3283594 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32835942012-02-23 De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids Li, Haiyan Dong, Yuanyuan Yang, Jing Liu, Xiuming Wang, Yanfang Yao, Na Guan, Lili Wang, Nan Wu, Jinyu Li, Xiaokun PLoS One Research Article Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome consisting of 153,769 unigenes. We annotated 82,916 of the unigenes with gene annotation and assigned functional terms and specific pathways to a subset of them. Metabolic pathway analysis revealed that 23 unigenes were predicted to be responsible for the biosynthesis of flavonoids and 8 were characterized as seed-specific oleosins. In addition, a large number of differentially expressed unigenes, for example, those annotated as participating in anthocyanin and chalcone synthesis, were predicted to be involved in flavonoid biosynthesis pathways. In conclusion, the de novo transcriptome investigation of the unique transcripts provided candidate gene resources for studying oleosin-coding genes and for investigating genes related to flavonoid biosynthesis and metabolism in safflower. Public Library of Science 2012-02-21 /pmc/articles/PMC3283594/ /pubmed/22363528 http://dx.doi.org/10.1371/journal.pone.0030987 Text en Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Haiyan Dong, Yuanyuan Yang, Jing Liu, Xiuming Wang, Yanfang Yao, Na Guan, Lili Wang, Nan Wu, Jinyu Li, Xiaokun De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids |
title |
De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids |
title_full |
De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids |
title_fullStr |
De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids |
title_full_unstemmed |
De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids |
title_short |
De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids |
title_sort | de novo transcriptome of safflower and the identification of putative genes for oleosin and the biosynthesis of flavonoids |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283594/ https://www.ncbi.nlm.nih.gov/pubmed/22363528 http://dx.doi.org/10.1371/journal.pone.0030987 |
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