The Clathrin Assembly Protein PICALM Is Required for Erythroid Maturation and Transferrin Internalization in Mice

Phosphatidylinositol binding clathrin assembly protein (PICALM), also known as clathrin assembly lymphoid myeloid leukemia protein (CALM), was originally isolated as part of the fusion gene CALM/AF10, which results from the chromosomal translocation t(10;11)(p13;q14). CALM is sufficient to drive cla...

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Autores principales: Suzuki, Mai, Tanaka, Hirokazu, Tanimura, Akira, Tanabe, Kenji, Oe, Natsuko, Rai, Shinya, Kon, Syunsuke, Fukumoto, Manabu, Takei, Kohji, Abe, Takaya, Matsumura, Itaru, Kanakura, Yuzuru, Watanabe, Toshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283694/
https://www.ncbi.nlm.nih.gov/pubmed/22363754
http://dx.doi.org/10.1371/journal.pone.0031854
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author Suzuki, Mai
Tanaka, Hirokazu
Tanimura, Akira
Tanabe, Kenji
Oe, Natsuko
Rai, Shinya
Kon, Syunsuke
Fukumoto, Manabu
Takei, Kohji
Abe, Takaya
Matsumura, Itaru
Kanakura, Yuzuru
Watanabe, Toshio
author_facet Suzuki, Mai
Tanaka, Hirokazu
Tanimura, Akira
Tanabe, Kenji
Oe, Natsuko
Rai, Shinya
Kon, Syunsuke
Fukumoto, Manabu
Takei, Kohji
Abe, Takaya
Matsumura, Itaru
Kanakura, Yuzuru
Watanabe, Toshio
author_sort Suzuki, Mai
collection PubMed
description Phosphatidylinositol binding clathrin assembly protein (PICALM), also known as clathrin assembly lymphoid myeloid leukemia protein (CALM), was originally isolated as part of the fusion gene CALM/AF10, which results from the chromosomal translocation t(10;11)(p13;q14). CALM is sufficient to drive clathrin assembly in vitro on lipid monolayers and regulates clathrin-coated budding and the size and shape of the vesicles at the plasma membrane. However, the physiological role of CALM has yet to be elucidated. Here, the role of CALM in vivo was investigated using CALM-deficient mice. CALM-deficient mice exhibited retarded growth in utero and were dwarfed throughout their shortened life-spans. Moreover, CALM-deficient mice suffered from severe anemia, and the maturation and iron content in erythroid precursors were severely impaired. CALM-deficient erythroid cells and embryonic fibroblasts exhibited impaired clathrin-mediated endocytosis of transferrin. These results indicate that CALM is required for erythroid maturation and transferrin internalization in mice.
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spelling pubmed-32836942012-02-23 The Clathrin Assembly Protein PICALM Is Required for Erythroid Maturation and Transferrin Internalization in Mice Suzuki, Mai Tanaka, Hirokazu Tanimura, Akira Tanabe, Kenji Oe, Natsuko Rai, Shinya Kon, Syunsuke Fukumoto, Manabu Takei, Kohji Abe, Takaya Matsumura, Itaru Kanakura, Yuzuru Watanabe, Toshio PLoS One Research Article Phosphatidylinositol binding clathrin assembly protein (PICALM), also known as clathrin assembly lymphoid myeloid leukemia protein (CALM), was originally isolated as part of the fusion gene CALM/AF10, which results from the chromosomal translocation t(10;11)(p13;q14). CALM is sufficient to drive clathrin assembly in vitro on lipid monolayers and regulates clathrin-coated budding and the size and shape of the vesicles at the plasma membrane. However, the physiological role of CALM has yet to be elucidated. Here, the role of CALM in vivo was investigated using CALM-deficient mice. CALM-deficient mice exhibited retarded growth in utero and were dwarfed throughout their shortened life-spans. Moreover, CALM-deficient mice suffered from severe anemia, and the maturation and iron content in erythroid precursors were severely impaired. CALM-deficient erythroid cells and embryonic fibroblasts exhibited impaired clathrin-mediated endocytosis of transferrin. These results indicate that CALM is required for erythroid maturation and transferrin internalization in mice. Public Library of Science 2012-02-21 /pmc/articles/PMC3283694/ /pubmed/22363754 http://dx.doi.org/10.1371/journal.pone.0031854 Text en Suzuki et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Suzuki, Mai
Tanaka, Hirokazu
Tanimura, Akira
Tanabe, Kenji
Oe, Natsuko
Rai, Shinya
Kon, Syunsuke
Fukumoto, Manabu
Takei, Kohji
Abe, Takaya
Matsumura, Itaru
Kanakura, Yuzuru
Watanabe, Toshio
The Clathrin Assembly Protein PICALM Is Required for Erythroid Maturation and Transferrin Internalization in Mice
title The Clathrin Assembly Protein PICALM Is Required for Erythroid Maturation and Transferrin Internalization in Mice
title_full The Clathrin Assembly Protein PICALM Is Required for Erythroid Maturation and Transferrin Internalization in Mice
title_fullStr The Clathrin Assembly Protein PICALM Is Required for Erythroid Maturation and Transferrin Internalization in Mice
title_full_unstemmed The Clathrin Assembly Protein PICALM Is Required for Erythroid Maturation and Transferrin Internalization in Mice
title_short The Clathrin Assembly Protein PICALM Is Required for Erythroid Maturation and Transferrin Internalization in Mice
title_sort clathrin assembly protein picalm is required for erythroid maturation and transferrin internalization in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283694/
https://www.ncbi.nlm.nih.gov/pubmed/22363754
http://dx.doi.org/10.1371/journal.pone.0031854
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