Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes

Vaccinia Tian Tan (VTT) was attenuated by deletion of the TC7L-TK2L and TA35R genes to generate MVTT3. The mutant was generated by replacing the open reading frames by a gene encoding enhanced green fluorescent protein (EGFP) flanked by loxP sites. Viruses expressing EGFP were then screened for and...

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Detalles Bibliográficos
Autores principales: Kan, Shifu, Wang, Yuhang, Sun, Lili, Jia, Peng, Qi, Yanxin, Su, Jiaqiang, Liu, Lei, Yang, Guohua, Liu, Liming, Wang, Zhuoyue, Wang, Jinhui, Liu, Guangchen, Jin, Ningyi, Li, Xiao, Ding, Zhuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283712/
https://www.ncbi.nlm.nih.gov/pubmed/22363781
http://dx.doi.org/10.1371/journal.pone.0031979
Descripción
Sumario:Vaccinia Tian Tan (VTT) was attenuated by deletion of the TC7L-TK2L and TA35R genes to generate MVTT3. The mutant was generated by replacing the open reading frames by a gene encoding enhanced green fluorescent protein (EGFP) flanked by loxP sites. Viruses expressing EGFP were then screened for and purified by serial plaque formation. In a second step the marker EGFP gene was removed by transfecting cells with a plasmid encoding cre recombinase and selecting for viruses that had lost the EGFP phenotype. The MVTT3 mutant was shown to be avirulent and immunogenic. These results support the conclusion that TC7L-TK2L and TA35R deletion mutants can be used as safe viral vectors or as platform for vaccines.

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