A Quick, Cost-Free Method of Purification of DNA Fragments from Agarose Gel

In this short communication we report a quick, cost-free method of purification of DNA fragments from agarose gel. Unlike those procedures that involve commercial kits, this method uses glass wool or absorbent cotton to filter agarose gel during a quick spinning-down of DNA, thus significantly simpl...

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Detalles Bibliográficos
Autores principales: Sun, Yuan, Sriramajayam, Kannappan, Luo, Dianzhong, Liao, D. Joshua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2012
Materias:
Short Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283835/
https://www.ncbi.nlm.nih.gov/pubmed/22359530
http://dx.doi.org/10.7150/jca.4163
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author Sun, Yuan
Sriramajayam, Kannappan
Luo, Dianzhong
Liao, D. Joshua
author_facet Sun, Yuan
Sriramajayam, Kannappan
Luo, Dianzhong
Liao, D. Joshua
author_sort Sun, Yuan
collection PubMed
description In this short communication we report a quick, cost-free method of purification of DNA fragments from agarose gel. Unlike those procedures that involve commercial kits, this method uses glass wool or absorbent cotton to filter agarose gel during a quick spinning-down of DNA, thus significantly simplifying the routine practice of many molecular biologists and decreasing the cost.
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spelling pubmed-32838352012-02-22 A Quick, Cost-Free Method of Purification of DNA Fragments from Agarose Gel Sun, Yuan Sriramajayam, Kannappan Luo, Dianzhong Liao, D. Joshua J Cancer Short Research Communication In this short communication we report a quick, cost-free method of purification of DNA fragments from agarose gel. Unlike those procedures that involve commercial kits, this method uses glass wool or absorbent cotton to filter agarose gel during a quick spinning-down of DNA, thus significantly simplifying the routine practice of many molecular biologists and decreasing the cost. Ivyspring International Publisher 2012-02-13 /pmc/articles/PMC3283835/ /pubmed/22359530 http://dx.doi.org/10.7150/jca.4163 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Short Research Communication
Sun, Yuan
Sriramajayam, Kannappan
Luo, Dianzhong
Liao, D. Joshua
A Quick, Cost-Free Method of Purification of DNA Fragments from Agarose Gel
title A Quick, Cost-Free Method of Purification of DNA Fragments from Agarose Gel
title_full A Quick, Cost-Free Method of Purification of DNA Fragments from Agarose Gel
title_fullStr A Quick, Cost-Free Method of Purification of DNA Fragments from Agarose Gel
title_full_unstemmed A Quick, Cost-Free Method of Purification of DNA Fragments from Agarose Gel
title_short A Quick, Cost-Free Method of Purification of DNA Fragments from Agarose Gel
title_sort quick, cost-free method of purification of dna fragments from agarose gel
topic Short Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283835/
https://www.ncbi.nlm.nih.gov/pubmed/22359530
http://dx.doi.org/10.7150/jca.4163
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