A Simple Assay Method for Melanosome Transfer

Pigmentation is induced by production of melanin in specialized organelles termed melanosomes and by transfer of these organelles from melanocytes to surrounding keratinocytes. The chemical basis of melanogenesis is relatively well known but the mechanism of melanosome transfer is not well studied....

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Detalles Bibliográficos
Autores principales: Choi, Hye Ryung, Park, So-Hee, Choi, Jae Woo, Kim, Dong-Seok, Park, Kyung Chan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Dermatological Association; The Korean Society for Investigative Dermatology 2012
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283861/
https://www.ncbi.nlm.nih.gov/pubmed/22363165
http://dx.doi.org/10.5021/ad.2012.24.1.90
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author Choi, Hye Ryung
Park, So-Hee
Choi, Jae Woo
Kim, Dong-Seok
Park, Kyung Chan
author_facet Choi, Hye Ryung
Park, So-Hee
Choi, Jae Woo
Kim, Dong-Seok
Park, Kyung Chan
author_sort Choi, Hye Ryung
collection PubMed
description Pigmentation is induced by production of melanin in specialized organelles termed melanosomes and by transfer of these organelles from melanocytes to surrounding keratinocytes. The chemical basis of melanogenesis is relatively well known but the mechanism of melanosome transfer is not well studied. Various pigmentary disorders and cosmetic applications require the use of depigmenting agents. Currently available topical agents used for the reduction of pigmentation mainly include tyrosinase inhibitors and/or melanocyte-cytotoxic agents. Recently, several agents have been introduced to inhibit melanosome transfer from melanocytes to keratinocytes. However, an experimental model for melanosome transfer is not well established. In this study, a simple assay method using flow cytometry is described.
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spelling pubmed-32838612012-02-23 A Simple Assay Method for Melanosome Transfer Choi, Hye Ryung Park, So-Hee Choi, Jae Woo Kim, Dong-Seok Park, Kyung Chan Ann Dermatol Short Report Pigmentation is induced by production of melanin in specialized organelles termed melanosomes and by transfer of these organelles from melanocytes to surrounding keratinocytes. The chemical basis of melanogenesis is relatively well known but the mechanism of melanosome transfer is not well studied. Various pigmentary disorders and cosmetic applications require the use of depigmenting agents. Currently available topical agents used for the reduction of pigmentation mainly include tyrosinase inhibitors and/or melanocyte-cytotoxic agents. Recently, several agents have been introduced to inhibit melanosome transfer from melanocytes to keratinocytes. However, an experimental model for melanosome transfer is not well established. In this study, a simple assay method using flow cytometry is described. Korean Dermatological Association; The Korean Society for Investigative Dermatology 2012-02 2012-02-02 /pmc/articles/PMC3283861/ /pubmed/22363165 http://dx.doi.org/10.5021/ad.2012.24.1.90 Text en Copyright © 2012 Korean Dermatological Association; The Korean Society for Investigative Dermatology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Choi, Hye Ryung
Park, So-Hee
Choi, Jae Woo
Kim, Dong-Seok
Park, Kyung Chan
A Simple Assay Method for Melanosome Transfer
title A Simple Assay Method for Melanosome Transfer
title_full A Simple Assay Method for Melanosome Transfer
title_fullStr A Simple Assay Method for Melanosome Transfer
title_full_unstemmed A Simple Assay Method for Melanosome Transfer
title_short A Simple Assay Method for Melanosome Transfer
title_sort simple assay method for melanosome transfer
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283861/
https://www.ncbi.nlm.nih.gov/pubmed/22363165
http://dx.doi.org/10.5021/ad.2012.24.1.90
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