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Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals
AIM: The aim of this study is to evaluate the bactericidal effect of 908 nm diode laser in conjunction with various irrigation regimes in disinfection of apical third of root dentin. MATERIALS AND METHODS: Sixty prepared teeth with single canals were contaminated with Enterococcus faecalis. The spec...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284013/ https://www.ncbi.nlm.nih.gov/pubmed/22368335 http://dx.doi.org/10.4103/0972-0707.92606 |
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author | Preethee, Thomas Kandaswamy, Deivanayagam Arathi, Ganesh Hannah, Rosaline |
author_facet | Preethee, Thomas Kandaswamy, Deivanayagam Arathi, Ganesh Hannah, Rosaline |
author_sort | Preethee, Thomas |
collection | PubMed |
description | AIM: The aim of this study is to evaluate the bactericidal effect of 908 nm diode laser in conjunction with various irrigation regimes in disinfection of apical third of root dentin. MATERIALS AND METHODS: Sixty prepared teeth with single canals were contaminated with Enterococcus faecalis. The specimens were divided into 6 groups (n = 10): Group 1 and 3 and 5 were subjected to chemo-mechanical preparation using 5.25% sodium hypochlorite (NaOCl), 17% Ethylenediaminetetraacetic acid (EDTA); 1.3% NaOCl, MTAD (mixture of doxycycline, citric acid and a detergent (Tween 80); and, 8.5% saline, respectively followed by 908 nm diode laser irradiation; Group 2 and 4, followed the same procedure as Group1 and 3, however without laser irradiation; and, Group 6, rinsed with saline solution (control). Dentin shavings from apical third were analyzed for the presence of E. faecalis using culture method and Polymerase Chain reaction (PCR). RESULTS: One-way Analysis of variance showed statistically significant differences between the laser irradiated groups, non irradiated groups and the control group. CONCLUSION: 908 nm diode used in conjunction with conventional chemomechanical techniques demonstrated a significant elimination of E. faecalis in the apical third of root dentin. |
format | Online Article Text |
id | pubmed-3284013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-32840132012-02-24 Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals Preethee, Thomas Kandaswamy, Deivanayagam Arathi, Ganesh Hannah, Rosaline J Conserv Dent Original Article AIM: The aim of this study is to evaluate the bactericidal effect of 908 nm diode laser in conjunction with various irrigation regimes in disinfection of apical third of root dentin. MATERIALS AND METHODS: Sixty prepared teeth with single canals were contaminated with Enterococcus faecalis. The specimens were divided into 6 groups (n = 10): Group 1 and 3 and 5 were subjected to chemo-mechanical preparation using 5.25% sodium hypochlorite (NaOCl), 17% Ethylenediaminetetraacetic acid (EDTA); 1.3% NaOCl, MTAD (mixture of doxycycline, citric acid and a detergent (Tween 80); and, 8.5% saline, respectively followed by 908 nm diode laser irradiation; Group 2 and 4, followed the same procedure as Group1 and 3, however without laser irradiation; and, Group 6, rinsed with saline solution (control). Dentin shavings from apical third were analyzed for the presence of E. faecalis using culture method and Polymerase Chain reaction (PCR). RESULTS: One-way Analysis of variance showed statistically significant differences between the laser irradiated groups, non irradiated groups and the control group. CONCLUSION: 908 nm diode used in conjunction with conventional chemomechanical techniques demonstrated a significant elimination of E. faecalis in the apical third of root dentin. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3284013/ /pubmed/22368335 http://dx.doi.org/10.4103/0972-0707.92606 Text en Copyright: © Journal of Conservative Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Preethee, Thomas Kandaswamy, Deivanayagam Arathi, Ganesh Hannah, Rosaline Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals |
title | Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals |
title_full | Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals |
title_fullStr | Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals |
title_full_unstemmed | Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals |
title_short | Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals |
title_sort | bactericidal effect of the 908 nm diode laser on enterococcus faecalis in infected root canals |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284013/ https://www.ncbi.nlm.nih.gov/pubmed/22368335 http://dx.doi.org/10.4103/0972-0707.92606 |
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