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Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals

AIM: The aim of this study is to evaluate the bactericidal effect of 908 nm diode laser in conjunction with various irrigation regimes in disinfection of apical third of root dentin. MATERIALS AND METHODS: Sixty prepared teeth with single canals were contaminated with Enterococcus faecalis. The spec...

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Autores principales: Preethee, Thomas, Kandaswamy, Deivanayagam, Arathi, Ganesh, Hannah, Rosaline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284013/
https://www.ncbi.nlm.nih.gov/pubmed/22368335
http://dx.doi.org/10.4103/0972-0707.92606
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author Preethee, Thomas
Kandaswamy, Deivanayagam
Arathi, Ganesh
Hannah, Rosaline
author_facet Preethee, Thomas
Kandaswamy, Deivanayagam
Arathi, Ganesh
Hannah, Rosaline
author_sort Preethee, Thomas
collection PubMed
description AIM: The aim of this study is to evaluate the bactericidal effect of 908 nm diode laser in conjunction with various irrigation regimes in disinfection of apical third of root dentin. MATERIALS AND METHODS: Sixty prepared teeth with single canals were contaminated with Enterococcus faecalis. The specimens were divided into 6 groups (n = 10): Group 1 and 3 and 5 were subjected to chemo-mechanical preparation using 5.25% sodium hypochlorite (NaOCl), 17% Ethylenediaminetetraacetic acid (EDTA); 1.3% NaOCl, MTAD (mixture of doxycycline, citric acid and a detergent (Tween 80); and, 8.5% saline, respectively followed by 908 nm diode laser irradiation; Group 2 and 4, followed the same procedure as Group1 and 3, however without laser irradiation; and, Group 6, rinsed with saline solution (control). Dentin shavings from apical third were analyzed for the presence of E. faecalis using culture method and Polymerase Chain reaction (PCR). RESULTS: One-way Analysis of variance showed statistically significant differences between the laser irradiated groups, non irradiated groups and the control group. CONCLUSION: 908 nm diode used in conjunction with conventional chemomechanical techniques demonstrated a significant elimination of E. faecalis in the apical third of root dentin.
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spelling pubmed-32840132012-02-24 Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals Preethee, Thomas Kandaswamy, Deivanayagam Arathi, Ganesh Hannah, Rosaline J Conserv Dent Original Article AIM: The aim of this study is to evaluate the bactericidal effect of 908 nm diode laser in conjunction with various irrigation regimes in disinfection of apical third of root dentin. MATERIALS AND METHODS: Sixty prepared teeth with single canals were contaminated with Enterococcus faecalis. The specimens were divided into 6 groups (n = 10): Group 1 and 3 and 5 were subjected to chemo-mechanical preparation using 5.25% sodium hypochlorite (NaOCl), 17% Ethylenediaminetetraacetic acid (EDTA); 1.3% NaOCl, MTAD (mixture of doxycycline, citric acid and a detergent (Tween 80); and, 8.5% saline, respectively followed by 908 nm diode laser irradiation; Group 2 and 4, followed the same procedure as Group1 and 3, however without laser irradiation; and, Group 6, rinsed with saline solution (control). Dentin shavings from apical third were analyzed for the presence of E. faecalis using culture method and Polymerase Chain reaction (PCR). RESULTS: One-way Analysis of variance showed statistically significant differences between the laser irradiated groups, non irradiated groups and the control group. CONCLUSION: 908 nm diode used in conjunction with conventional chemomechanical techniques demonstrated a significant elimination of E. faecalis in the apical third of root dentin. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3284013/ /pubmed/22368335 http://dx.doi.org/10.4103/0972-0707.92606 Text en Copyright: © Journal of Conservative Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Preethee, Thomas
Kandaswamy, Deivanayagam
Arathi, Ganesh
Hannah, Rosaline
Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals
title Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals
title_full Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals
title_fullStr Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals
title_full_unstemmed Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals
title_short Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals
title_sort bactericidal effect of the 908 nm diode laser on enterococcus faecalis in infected root canals
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284013/
https://www.ncbi.nlm.nih.gov/pubmed/22368335
http://dx.doi.org/10.4103/0972-0707.92606
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