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Delayed maturation and altered proliferation within the rat rostral migratory stream following maternal deprivation

The objective of this study was to investigate whether stressful experience during early postnatal period may influence morphological characteristics of the rat neurogenic pathway – the rostral migratory stream (RMS) and proliferation of neuronal precursors in three successive areas of the RMS: in t...

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Autores principales: Lievajová, K., Blaško, J., Martončíková, M., Cigánková, V., Račeková, E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284235/
https://www.ncbi.nlm.nih.gov/pubmed/22297439
http://dx.doi.org/10.4081/ejh.2011.e33
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author Lievajová, K.
Blaško, J.
Martončíková, M.
Cigánková, V.
Račeková, E.
author_facet Lievajová, K.
Blaško, J.
Martončíková, M.
Cigánková, V.
Račeková, E.
author_sort Lievajová, K.
collection PubMed
description The objective of this study was to investigate whether stressful experience during early postnatal period may influence morphological characteristics of the rat neurogenic pathway – the rostral migratory stream (RMS) and proliferation of neuronal precursors in three successive areas of the RMS: in the vertical arm, the elbow and the horizontal arm. To induce stress, the pups were subjected to repeated maternal deprivation during the first postnatal week after birth. Brains were analyzed at the seventh postnatal day. The controls matched the age of maternally deprived animals. Observation of hematoxylin-eosin stained sections showed that maternal deprivation did not affect the general morphological appearance of the RMS. The shape of the RMS of maternally deprived rats resembles the RMS of control animals. Maternal deprivation caused slight, not significant increase in the RMS thickness in comparison with control rats. Significant difference between the control and maternally deprived rats concerns the olfactory ventricle. While in seven days old control rats the olfactory ventricle is completely closed, in maternally deprived rats of the same age the olfactory ventricle was regularly visible as a narrow lumen at the axis of the RMS horizontal arm. This finding indicates delayed maturation of the migratory pathway as a consequence of stress. Proliferation activity has been assessed by immunoreactivity of the endogenous cell cycle protein Ki-67. The results of Ki-67 immunohistochemistry showed that seven days' maternal separation for 3 h daily induces significant quantitative changes in the number of proliferating cells within the RMS. The response of Ki-67-positive cells to stress differed in individual part of the RMS, with a marked decrease in the vertical arm and a significant increase in the elbow, suggesting heterogeneity of neural stem cells along the RMS; while in the RMS vertical arm the number of dividing cells significantly decreased, there was a marked increase of Ki-67-positive cells in the RMS elbow. This suggests heterogeneity of neural stem cells along the RMS.
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spelling pubmed-32842352012-02-23 Delayed maturation and altered proliferation within the rat rostral migratory stream following maternal deprivation Lievajová, K. Blaško, J. Martončíková, M. Cigánková, V. Račeková, E. Eur J Histochem Original Paper The objective of this study was to investigate whether stressful experience during early postnatal period may influence morphological characteristics of the rat neurogenic pathway – the rostral migratory stream (RMS) and proliferation of neuronal precursors in three successive areas of the RMS: in the vertical arm, the elbow and the horizontal arm. To induce stress, the pups were subjected to repeated maternal deprivation during the first postnatal week after birth. Brains were analyzed at the seventh postnatal day. The controls matched the age of maternally deprived animals. Observation of hematoxylin-eosin stained sections showed that maternal deprivation did not affect the general morphological appearance of the RMS. The shape of the RMS of maternally deprived rats resembles the RMS of control animals. Maternal deprivation caused slight, not significant increase in the RMS thickness in comparison with control rats. Significant difference between the control and maternally deprived rats concerns the olfactory ventricle. While in seven days old control rats the olfactory ventricle is completely closed, in maternally deprived rats of the same age the olfactory ventricle was regularly visible as a narrow lumen at the axis of the RMS horizontal arm. This finding indicates delayed maturation of the migratory pathway as a consequence of stress. Proliferation activity has been assessed by immunoreactivity of the endogenous cell cycle protein Ki-67. The results of Ki-67 immunohistochemistry showed that seven days' maternal separation for 3 h daily induces significant quantitative changes in the number of proliferating cells within the RMS. The response of Ki-67-positive cells to stress differed in individual part of the RMS, with a marked decrease in the vertical arm and a significant increase in the elbow, suggesting heterogeneity of neural stem cells along the RMS; while in the RMS vertical arm the number of dividing cells significantly decreased, there was a marked increase of Ki-67-positive cells in the RMS elbow. This suggests heterogeneity of neural stem cells along the RMS. PAGEPress Publications 2011-10-19 /pmc/articles/PMC3284235/ /pubmed/22297439 http://dx.doi.org/10.4081/ejh.2011.e33 Text en ©Copyright K. Lievajová et al., 2011 This work is licensed under a Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0). Licensee PAGEPress, Italy
spellingShingle Original Paper
Lievajová, K.
Blaško, J.
Martončíková, M.
Cigánková, V.
Račeková, E.
Delayed maturation and altered proliferation within the rat rostral migratory stream following maternal deprivation
title Delayed maturation and altered proliferation within the rat rostral migratory stream following maternal deprivation
title_full Delayed maturation and altered proliferation within the rat rostral migratory stream following maternal deprivation
title_fullStr Delayed maturation and altered proliferation within the rat rostral migratory stream following maternal deprivation
title_full_unstemmed Delayed maturation and altered proliferation within the rat rostral migratory stream following maternal deprivation
title_short Delayed maturation and altered proliferation within the rat rostral migratory stream following maternal deprivation
title_sort delayed maturation and altered proliferation within the rat rostral migratory stream following maternal deprivation
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284235/
https://www.ncbi.nlm.nih.gov/pubmed/22297439
http://dx.doi.org/10.4081/ejh.2011.e33
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