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Accuracy of a Commercial IgM ELISA for the Diagnosis of Human Leptospirosis in Thailand

The Leptospira immunoglobulin M enzyme-linked immunosorbent assay (IgM ELISA) has been recommended for the rapid diagnosis of leptospirosis in endemic areas. We conducted a retrospective case-control study of 218 patients (109 leptospirosis cases confirmed by Leptospira culture and/or microscopic ag...

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Autores principales: Desakorn, Varunee, Wuthiekanun, Vanaporn, Thanachartwet, Vipa, Sahassananda, Duangjai, Chierakul, Wirongrong, Apiwattanaporn, Apichat, Day, Nicholas P., Limmathurotsakul, Direk, Peacock, Sharon J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society of Tropical Medicine and Hygiene 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284374/
https://www.ncbi.nlm.nih.gov/pubmed/22403329
http://dx.doi.org/10.4269/ajtmh.2012.11-0423
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author Desakorn, Varunee
Wuthiekanun, Vanaporn
Thanachartwet, Vipa
Sahassananda, Duangjai
Chierakul, Wirongrong
Apiwattanaporn, Apichat
Day, Nicholas P.
Limmathurotsakul, Direk
Peacock, Sharon J.
author_facet Desakorn, Varunee
Wuthiekanun, Vanaporn
Thanachartwet, Vipa
Sahassananda, Duangjai
Chierakul, Wirongrong
Apiwattanaporn, Apichat
Day, Nicholas P.
Limmathurotsakul, Direk
Peacock, Sharon J.
author_sort Desakorn, Varunee
collection PubMed
description The Leptospira immunoglobulin M enzyme-linked immunosorbent assay (IgM ELISA) has been recommended for the rapid diagnosis of leptospirosis in endemic areas. We conducted a retrospective case-control study of 218 patients (109 leptospirosis cases confirmed by Leptospira culture and/or microscopic agglutination test and 109 control patients with acute febrile illness) to evaluate the diagnostic accuracy of a commercial IgM ELISA (Panbio) in northeast Thailand. Paired serum samples taken on admission and at least 10 days after the onset of symptoms were tested. Using the cutoff value recommended by the manufacturer (11 Panbio units), sensitivity and specificity of IgM ELISA on paired sera were 90.8% and 55.1%. A receiver operating characteristic curve was used to determine the optimal cutoff value. This was 20 Panbio units, which gave a sensitivity and specificity of 76.1% and 82.6%, respectively, on paired sera. We conclude that using either cutoff value, the accuracy of IgM ELISA is limited in our setting.
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spelling pubmed-32843742012-03-23 Accuracy of a Commercial IgM ELISA for the Diagnosis of Human Leptospirosis in Thailand Desakorn, Varunee Wuthiekanun, Vanaporn Thanachartwet, Vipa Sahassananda, Duangjai Chierakul, Wirongrong Apiwattanaporn, Apichat Day, Nicholas P. Limmathurotsakul, Direk Peacock, Sharon J. Am J Trop Med Hyg Articles The Leptospira immunoglobulin M enzyme-linked immunosorbent assay (IgM ELISA) has been recommended for the rapid diagnosis of leptospirosis in endemic areas. We conducted a retrospective case-control study of 218 patients (109 leptospirosis cases confirmed by Leptospira culture and/or microscopic agglutination test and 109 control patients with acute febrile illness) to evaluate the diagnostic accuracy of a commercial IgM ELISA (Panbio) in northeast Thailand. Paired serum samples taken on admission and at least 10 days after the onset of symptoms were tested. Using the cutoff value recommended by the manufacturer (11 Panbio units), sensitivity and specificity of IgM ELISA on paired sera were 90.8% and 55.1%. A receiver operating characteristic curve was used to determine the optimal cutoff value. This was 20 Panbio units, which gave a sensitivity and specificity of 76.1% and 82.6%, respectively, on paired sera. We conclude that using either cutoff value, the accuracy of IgM ELISA is limited in our setting. The American Society of Tropical Medicine and Hygiene 2012-03-01 /pmc/articles/PMC3284374/ /pubmed/22403329 http://dx.doi.org/10.4269/ajtmh.2012.11-0423 Text en ©The American Society of Tropical Medicine and Hygiene This is an Open Access article distributed under the terms and of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Desakorn, Varunee
Wuthiekanun, Vanaporn
Thanachartwet, Vipa
Sahassananda, Duangjai
Chierakul, Wirongrong
Apiwattanaporn, Apichat
Day, Nicholas P.
Limmathurotsakul, Direk
Peacock, Sharon J.
Accuracy of a Commercial IgM ELISA for the Diagnosis of Human Leptospirosis in Thailand
title Accuracy of a Commercial IgM ELISA for the Diagnosis of Human Leptospirosis in Thailand
title_full Accuracy of a Commercial IgM ELISA for the Diagnosis of Human Leptospirosis in Thailand
title_fullStr Accuracy of a Commercial IgM ELISA for the Diagnosis of Human Leptospirosis in Thailand
title_full_unstemmed Accuracy of a Commercial IgM ELISA for the Diagnosis of Human Leptospirosis in Thailand
title_short Accuracy of a Commercial IgM ELISA for the Diagnosis of Human Leptospirosis in Thailand
title_sort accuracy of a commercial igm elisa for the diagnosis of human leptospirosis in thailand
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284374/
https://www.ncbi.nlm.nih.gov/pubmed/22403329
http://dx.doi.org/10.4269/ajtmh.2012.11-0423
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