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Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells

BACKGROUND: Elevated systemic levels of pro-inflammatory cytokines cause hypotension during septic shock and induce capillary leakage in acute lung injury. Manassantin B has anti-inflammatory and anti-plasmoidal properties. This study examined the effects of manassantin B on lipopolysaccharide (LPS)...

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Autores principales: Park, Hwan Chul, Bae, Hong-Beom, Jeong, Cheol-Won, Lee, Seong Heon, Jeung, Hye Jin, Kwak, Sang-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Anesthesiologists 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284739/
https://www.ncbi.nlm.nih.gov/pubmed/22379572
http://dx.doi.org/10.4097/kjae.2012.62.2.161
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author Park, Hwan Chul
Bae, Hong-Beom
Jeong, Cheol-Won
Lee, Seong Heon
Jeung, Hye Jin
Kwak, Sang-Hyun
author_facet Park, Hwan Chul
Bae, Hong-Beom
Jeong, Cheol-Won
Lee, Seong Heon
Jeung, Hye Jin
Kwak, Sang-Hyun
author_sort Park, Hwan Chul
collection PubMed
description BACKGROUND: Elevated systemic levels of pro-inflammatory cytokines cause hypotension during septic shock and induce capillary leakage in acute lung injury. Manassantin B has anti-inflammatory and anti-plasmoidal properties. This study examined the effects of manassantin B on lipopolysaccharide (LPS)-induced inflammatory response in murine macrophages. METHODS: RAW 264.7 macrophage cells were incubated without or with (1, 3 and 10 µM) manassantin B and without or with (100 ng/ml) LPS. Manassantin B dissolved in phosphate buffered saline was added to the medium 1 h prior to the addition of LPS. The degree of activation of mitogen-activated protein kinase (MAPK) including extracellular signal-regulated kinases 1 and 2 (ERK1/2), c-Jun amino terminal kinases (JNK) and p38 MAPK, and the level of interleukin (IL)-1β were determined 30 min and 24 h after the addition of LPS respectively. RESULTS: Manassantin B inhibited the production of IL-1β and attenuated the phosphorylations of ERK1/2 and p38 MAPK, but not that of JNK, in RAW 264.7 cells treated with LPS. CONCLUSIONS: Manassantin B reduces LPS-induced IL-1β expression through effects on ERK1/2- and p38 MAPK-mediated pathways. Manassantin B has potential as a potent anti-inflammatory drug for use in pathological processes such as sepsis or acute lung injury.
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spelling pubmed-32847392012-02-29 Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells Park, Hwan Chul Bae, Hong-Beom Jeong, Cheol-Won Lee, Seong Heon Jeung, Hye Jin Kwak, Sang-Hyun Korean J Anesthesiol Experimental Research Article BACKGROUND: Elevated systemic levels of pro-inflammatory cytokines cause hypotension during septic shock and induce capillary leakage in acute lung injury. Manassantin B has anti-inflammatory and anti-plasmoidal properties. This study examined the effects of manassantin B on lipopolysaccharide (LPS)-induced inflammatory response in murine macrophages. METHODS: RAW 264.7 macrophage cells were incubated without or with (1, 3 and 10 µM) manassantin B and without or with (100 ng/ml) LPS. Manassantin B dissolved in phosphate buffered saline was added to the medium 1 h prior to the addition of LPS. The degree of activation of mitogen-activated protein kinase (MAPK) including extracellular signal-regulated kinases 1 and 2 (ERK1/2), c-Jun amino terminal kinases (JNK) and p38 MAPK, and the level of interleukin (IL)-1β were determined 30 min and 24 h after the addition of LPS respectively. RESULTS: Manassantin B inhibited the production of IL-1β and attenuated the phosphorylations of ERK1/2 and p38 MAPK, but not that of JNK, in RAW 264.7 cells treated with LPS. CONCLUSIONS: Manassantin B reduces LPS-induced IL-1β expression through effects on ERK1/2- and p38 MAPK-mediated pathways. Manassantin B has potential as a potent anti-inflammatory drug for use in pathological processes such as sepsis or acute lung injury. The Korean Society of Anesthesiologists 2012-02 2012-02-20 /pmc/articles/PMC3284739/ /pubmed/22379572 http://dx.doi.org/10.4097/kjae.2012.62.2.161 Text en Copyright © the Korean Society of Anesthesiologists, 2012 http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Experimental Research Article
Park, Hwan Chul
Bae, Hong-Beom
Jeong, Cheol-Won
Lee, Seong Heon
Jeung, Hye Jin
Kwak, Sang-Hyun
Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells
title Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells
title_full Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells
title_fullStr Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells
title_full_unstemmed Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells
title_short Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells
title_sort effect of manassantin b, a lignan isolated from saururus chinensis, on lipopolysaccharide-induced interleukin-1β in raw 264.7 cells
topic Experimental Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284739/
https://www.ncbi.nlm.nih.gov/pubmed/22379572
http://dx.doi.org/10.4097/kjae.2012.62.2.161
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