Cargando…

Retagging Identifies Dendritic Cell-specific Intercellular Adhesion Molecule-3 (ICAM3)-grabbing Non-integrin (DC-SIGN) Protein as a Novel Receptor for a Major Allergen from House Dust Mite

Dendritic cells (DCs) have been shown to play a key role in the initiation and maintenance of immune responses to microbial pathogens as well as to allergens, but the exact mechanisms of their involvement in allergic responses and Th2 cell differentiation have remained elusive. Using retagging, we i...

Descripción completa

Detalles Bibliográficos
Autores principales: Emara, Mohamed, Royer, Pierre-Joseph, Mahdavi, Jafar, Shakib, Farouk, Ghaemmaghami, Amir M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285347/
https://www.ncbi.nlm.nih.gov/pubmed/22205703
http://dx.doi.org/10.1074/jbc.M111.312520
_version_ 1782224476609445888
author Emara, Mohamed
Royer, Pierre-Joseph
Mahdavi, Jafar
Shakib, Farouk
Ghaemmaghami, Amir M.
author_facet Emara, Mohamed
Royer, Pierre-Joseph
Mahdavi, Jafar
Shakib, Farouk
Ghaemmaghami, Amir M.
author_sort Emara, Mohamed
collection PubMed
description Dendritic cells (DCs) have been shown to play a key role in the initiation and maintenance of immune responses to microbial pathogens as well as to allergens, but the exact mechanisms of their involvement in allergic responses and Th2 cell differentiation have remained elusive. Using retagging, we identified DC-SIGN as a novel receptor involved in the initial recognition and uptake of the major house dust mite and dog allergens Der p 1 and Can f 1, respectively. To confirm this, we used gene silencing to specifically inhibit DC-SIGN expression by DCs followed by allergen uptake studies. Binding and uptake of Der p 1 and Can f 1 allergens was assessed by ELISA and flow cytometry. Intriguingly, our data showed that silencing DC-SIGN on DCs promotes a Th2 phenotype in DC/T cell co-cultures. These findings should lead to better understanding of the molecular basis of allergen-induced Th2 cell polarization and in doing so paves the way for the rational design of novel intervention strategies by targeting allergen receptors on innate immune cells or their carbohydrate counterstructures on allergens.
format Online
Article
Text
id pubmed-3285347
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher American Society for Biochemistry and Molecular Biology
record_format MEDLINE/PubMed
spelling pubmed-32853472012-02-29 Retagging Identifies Dendritic Cell-specific Intercellular Adhesion Molecule-3 (ICAM3)-grabbing Non-integrin (DC-SIGN) Protein as a Novel Receptor for a Major Allergen from House Dust Mite Emara, Mohamed Royer, Pierre-Joseph Mahdavi, Jafar Shakib, Farouk Ghaemmaghami, Amir M. J Biol Chem Immunology Dendritic cells (DCs) have been shown to play a key role in the initiation and maintenance of immune responses to microbial pathogens as well as to allergens, but the exact mechanisms of their involvement in allergic responses and Th2 cell differentiation have remained elusive. Using retagging, we identified DC-SIGN as a novel receptor involved in the initial recognition and uptake of the major house dust mite and dog allergens Der p 1 and Can f 1, respectively. To confirm this, we used gene silencing to specifically inhibit DC-SIGN expression by DCs followed by allergen uptake studies. Binding and uptake of Der p 1 and Can f 1 allergens was assessed by ELISA and flow cytometry. Intriguingly, our data showed that silencing DC-SIGN on DCs promotes a Th2 phenotype in DC/T cell co-cultures. These findings should lead to better understanding of the molecular basis of allergen-induced Th2 cell polarization and in doing so paves the way for the rational design of novel intervention strategies by targeting allergen receptors on innate immune cells or their carbohydrate counterstructures on allergens. American Society for Biochemistry and Molecular Biology 2012-02-17 2011-12-28 /pmc/articles/PMC3285347/ /pubmed/22205703 http://dx.doi.org/10.1074/jbc.M111.312520 Text en © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articles
spellingShingle Immunology
Emara, Mohamed
Royer, Pierre-Joseph
Mahdavi, Jafar
Shakib, Farouk
Ghaemmaghami, Amir M.
Retagging Identifies Dendritic Cell-specific Intercellular Adhesion Molecule-3 (ICAM3)-grabbing Non-integrin (DC-SIGN) Protein as a Novel Receptor for a Major Allergen from House Dust Mite
title Retagging Identifies Dendritic Cell-specific Intercellular Adhesion Molecule-3 (ICAM3)-grabbing Non-integrin (DC-SIGN) Protein as a Novel Receptor for a Major Allergen from House Dust Mite
title_full Retagging Identifies Dendritic Cell-specific Intercellular Adhesion Molecule-3 (ICAM3)-grabbing Non-integrin (DC-SIGN) Protein as a Novel Receptor for a Major Allergen from House Dust Mite
title_fullStr Retagging Identifies Dendritic Cell-specific Intercellular Adhesion Molecule-3 (ICAM3)-grabbing Non-integrin (DC-SIGN) Protein as a Novel Receptor for a Major Allergen from House Dust Mite
title_full_unstemmed Retagging Identifies Dendritic Cell-specific Intercellular Adhesion Molecule-3 (ICAM3)-grabbing Non-integrin (DC-SIGN) Protein as a Novel Receptor for a Major Allergen from House Dust Mite
title_short Retagging Identifies Dendritic Cell-specific Intercellular Adhesion Molecule-3 (ICAM3)-grabbing Non-integrin (DC-SIGN) Protein as a Novel Receptor for a Major Allergen from House Dust Mite
title_sort retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (icam3)-grabbing non-integrin (dc-sign) protein as a novel receptor for a major allergen from house dust mite
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285347/
https://www.ncbi.nlm.nih.gov/pubmed/22205703
http://dx.doi.org/10.1074/jbc.M111.312520
work_keys_str_mv AT emaramohamed retaggingidentifiesdendriticcellspecificintercellularadhesionmolecule3icam3grabbingnonintegrindcsignproteinasanovelreceptorforamajorallergenfromhousedustmite
AT royerpierrejoseph retaggingidentifiesdendriticcellspecificintercellularadhesionmolecule3icam3grabbingnonintegrindcsignproteinasanovelreceptorforamajorallergenfromhousedustmite
AT mahdavijafar retaggingidentifiesdendriticcellspecificintercellularadhesionmolecule3icam3grabbingnonintegrindcsignproteinasanovelreceptorforamajorallergenfromhousedustmite
AT shakibfarouk retaggingidentifiesdendriticcellspecificintercellularadhesionmolecule3icam3grabbingnonintegrindcsignproteinasanovelreceptorforamajorallergenfromhousedustmite
AT ghaemmaghamiamirm retaggingidentifiesdendriticcellspecificintercellularadhesionmolecule3icam3grabbingnonintegrindcsignproteinasanovelreceptorforamajorallergenfromhousedustmite