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A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass
The conventional biochemical platform featuring enzymatic hydrolysis involves five key steps: pretreatment, cellulase production, enzymatic hydrolysis, fermentation, and product recovery. Sugars are produced as reactive intermediates for subsequent fermentation to fuels and chemicals. Herein, an alt...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285643/ https://www.ncbi.nlm.nih.gov/pubmed/22384058 http://dx.doi.org/10.1371/journal.pone.0031693 |
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author | Fan, Zhiliang Wu, Weihua Hildebrand, Amanda Kasuga, Takao Zhang, Ruifu Xiong, Xiaochao |
author_facet | Fan, Zhiliang Wu, Weihua Hildebrand, Amanda Kasuga, Takao Zhang, Ruifu Xiong, Xiaochao |
author_sort | Fan, Zhiliang |
collection | PubMed |
description | The conventional biochemical platform featuring enzymatic hydrolysis involves five key steps: pretreatment, cellulase production, enzymatic hydrolysis, fermentation, and product recovery. Sugars are produced as reactive intermediates for subsequent fermentation to fuels and chemicals. Herein, an alternative biochemical route is proposed. Pretreatment, enzymatic hydrolysis and cellulase production is consolidated into one single step, referred to as consolidated aerobic processing, and sugar aldonates are produced as the reactive intermediates for biofuels production by fermentation. In this study, we demonstrate the viability of consolidation of the enzymatic hydrolysis and cellulase production steps in the new route using Neurospora crassa as the model microorganism and the conversion of cellulose to ethanol as the model system. We intended to prove the two hypotheses: 1) cellulose can be directed to produce cellobionate by reducing β-glucosidase production and by enhancing cellobiose dehydrogenase production; and 2) both of the two hydrolysis products of cellobionate—glucose and gluconate—can be used as carbon sources for ethanol and other chemical production. Our results showed that knocking out multiple copies of β-glucosidase genes led to cellobionate production from cellulose, without jeopardizing the cellulose hydrolysis rate. Simulating cellobiose dehydrogenase over-expression by addition of exogenous cellobiose dehydrogenase led to more cellobionate production. Both of the two hydrolysis products of cellobionate: glucose and gluconate can be used by Escherichia coli KO 11 for efficient ethanol production. They were utilized simultaneously in glucose and gluconate co-fermentation. Gluconate was used even faster than glucose. The results support the viability of the two hypotheses that lay the foundation for the proposed new route. |
format | Online Article Text |
id | pubmed-3285643 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32856432012-03-01 A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass Fan, Zhiliang Wu, Weihua Hildebrand, Amanda Kasuga, Takao Zhang, Ruifu Xiong, Xiaochao PLoS One Research Article The conventional biochemical platform featuring enzymatic hydrolysis involves five key steps: pretreatment, cellulase production, enzymatic hydrolysis, fermentation, and product recovery. Sugars are produced as reactive intermediates for subsequent fermentation to fuels and chemicals. Herein, an alternative biochemical route is proposed. Pretreatment, enzymatic hydrolysis and cellulase production is consolidated into one single step, referred to as consolidated aerobic processing, and sugar aldonates are produced as the reactive intermediates for biofuels production by fermentation. In this study, we demonstrate the viability of consolidation of the enzymatic hydrolysis and cellulase production steps in the new route using Neurospora crassa as the model microorganism and the conversion of cellulose to ethanol as the model system. We intended to prove the two hypotheses: 1) cellulose can be directed to produce cellobionate by reducing β-glucosidase production and by enhancing cellobiose dehydrogenase production; and 2) both of the two hydrolysis products of cellobionate—glucose and gluconate—can be used as carbon sources for ethanol and other chemical production. Our results showed that knocking out multiple copies of β-glucosidase genes led to cellobionate production from cellulose, without jeopardizing the cellulose hydrolysis rate. Simulating cellobiose dehydrogenase over-expression by addition of exogenous cellobiose dehydrogenase led to more cellobionate production. Both of the two hydrolysis products of cellobionate: glucose and gluconate can be used by Escherichia coli KO 11 for efficient ethanol production. They were utilized simultaneously in glucose and gluconate co-fermentation. Gluconate was used even faster than glucose. The results support the viability of the two hypotheses that lay the foundation for the proposed new route. Public Library of Science 2012-02-23 /pmc/articles/PMC3285643/ /pubmed/22384058 http://dx.doi.org/10.1371/journal.pone.0031693 Text en Fan et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Fan, Zhiliang Wu, Weihua Hildebrand, Amanda Kasuga, Takao Zhang, Ruifu Xiong, Xiaochao A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass |
title | A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass |
title_full | A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass |
title_fullStr | A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass |
title_full_unstemmed | A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass |
title_short | A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass |
title_sort | novel biochemical route for fuels and chemicals production from cellulosic biomass |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285643/ https://www.ncbi.nlm.nih.gov/pubmed/22384058 http://dx.doi.org/10.1371/journal.pone.0031693 |
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