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GluA2-lacking AMPA receptors in hippocampal CA1 cell synapses: evidence from gene-targeted mice
The GluA2 subunit in heteromeric alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor channels restricts Ca(2+) permeability and block by polyamines, rendering linear the current-voltage relationship of these glutamate-gated cation channels. Although GluA2-lacking synaptic AMPA r...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285882/ https://www.ncbi.nlm.nih.gov/pubmed/22375105 http://dx.doi.org/10.3389/fnmol.2012.00022 |
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author | Rozov, Andrei Sprengel, Rolf Seeburg, Peter H. |
author_facet | Rozov, Andrei Sprengel, Rolf Seeburg, Peter H. |
author_sort | Rozov, Andrei |
collection | PubMed |
description | The GluA2 subunit in heteromeric alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor channels restricts Ca(2+) permeability and block by polyamines, rendering linear the current-voltage relationship of these glutamate-gated cation channels. Although GluA2-lacking synaptic AMPA receptors occur in GABA-ergic inhibitory neurons, hippocampal CA1 pyramidal cell synapses are widely held to feature only GluA2 containing AMPA receptors. A controversy has arisen from reports of GluA2-lacking AMPA receptors at hippocampal CA3-to-CA1 cell synapses and a study contesting these findings. Here we sought independent evidence for the presence of GluA2-lacking AMPA receptors in CA1 pyramidal cell synapses by probing the sensitivity of their gated cation channels in wild-type (WT) mice and gene-targeted mouse mutants to philanthotoxin, a specific blocker of GluA2-lacking AMPA receptors. The mutants either lacked GluA2 for maximal philanthotoxin sensitivity, or, for minimal sensitivity, expressed GluA1 solely in a Q/R site-edited version or not at all. Our comparative electrophysiological analyses provide incontrovertible evidence for the presence in wild-type CA1 pyramidal cell synapses of GluA2-less AMPA receptor channels. This article is part of a Special Issue entitled “Calcium permeable AMPARs in synaptic plasticity and disease.” |
format | Online Article Text |
id | pubmed-3285882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-32858822012-02-28 GluA2-lacking AMPA receptors in hippocampal CA1 cell synapses: evidence from gene-targeted mice Rozov, Andrei Sprengel, Rolf Seeburg, Peter H. Front Mol Neurosci Neuroscience The GluA2 subunit in heteromeric alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor channels restricts Ca(2+) permeability and block by polyamines, rendering linear the current-voltage relationship of these glutamate-gated cation channels. Although GluA2-lacking synaptic AMPA receptors occur in GABA-ergic inhibitory neurons, hippocampal CA1 pyramidal cell synapses are widely held to feature only GluA2 containing AMPA receptors. A controversy has arisen from reports of GluA2-lacking AMPA receptors at hippocampal CA3-to-CA1 cell synapses and a study contesting these findings. Here we sought independent evidence for the presence of GluA2-lacking AMPA receptors in CA1 pyramidal cell synapses by probing the sensitivity of their gated cation channels in wild-type (WT) mice and gene-targeted mouse mutants to philanthotoxin, a specific blocker of GluA2-lacking AMPA receptors. The mutants either lacked GluA2 for maximal philanthotoxin sensitivity, or, for minimal sensitivity, expressed GluA1 solely in a Q/R site-edited version or not at all. Our comparative electrophysiological analyses provide incontrovertible evidence for the presence in wild-type CA1 pyramidal cell synapses of GluA2-less AMPA receptor channels. This article is part of a Special Issue entitled “Calcium permeable AMPARs in synaptic plasticity and disease.” Frontiers Media S.A. 2012-02-24 /pmc/articles/PMC3285882/ /pubmed/22375105 http://dx.doi.org/10.3389/fnmol.2012.00022 Text en Copyright © 2012 Rozov, Sprengel and Seeburg. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited. |
spellingShingle | Neuroscience Rozov, Andrei Sprengel, Rolf Seeburg, Peter H. GluA2-lacking AMPA receptors in hippocampal CA1 cell synapses: evidence from gene-targeted mice |
title | GluA2-lacking AMPA receptors in hippocampal CA1 cell synapses: evidence from gene-targeted mice |
title_full | GluA2-lacking AMPA receptors in hippocampal CA1 cell synapses: evidence from gene-targeted mice |
title_fullStr | GluA2-lacking AMPA receptors in hippocampal CA1 cell synapses: evidence from gene-targeted mice |
title_full_unstemmed | GluA2-lacking AMPA receptors in hippocampal CA1 cell synapses: evidence from gene-targeted mice |
title_short | GluA2-lacking AMPA receptors in hippocampal CA1 cell synapses: evidence from gene-targeted mice |
title_sort | glua2-lacking ampa receptors in hippocampal ca1 cell synapses: evidence from gene-targeted mice |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285882/ https://www.ncbi.nlm.nih.gov/pubmed/22375105 http://dx.doi.org/10.3389/fnmol.2012.00022 |
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