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High-level ribosomal frameshifting directs the synthesis of IS150 gene products.
IS150 contains two tandem, out-of-phase, overlapping genes, ins150A and ins150B, which are controlled by the same promoter. These genes encode proteins of 19 and 31 kD, respectively. A third protein of 49 kD is a transframe gene product consisting of domains encoded by both genes. Specific -1 riboso...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
1991
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC328623/ https://www.ncbi.nlm.nih.gov/pubmed/1653413 |
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author | Vögele, K Schwartz, E Welz, C Schiltz, E Rak, B |
author_facet | Vögele, K Schwartz, E Welz, C Schiltz, E Rak, B |
author_sort | Vögele, K |
collection | PubMed |
description | IS150 contains two tandem, out-of-phase, overlapping genes, ins150A and ins150B, which are controlled by the same promoter. These genes encode proteins of 19 and 31 kD, respectively. A third protein of 49 kD is a transframe gene product consisting of domains encoded by both genes. Specific -1 ribosomal frameshifting is responsible for the synthesis of the large protein. Expression of ins150B also involves frameshifting. The IS150 frameshifting signals operate with a remarkably high efficiency, causing about one third of the ribosomes to switch frame. All of the signals required for this process are encoded in a 83-bp segment of the element. The heptanucleotide A AAA AAG and a potential stem-loop-forming sequence mark the frameshifting site. Similar sequence elements are found in -1 frameshifting regions of bacterial and retroviral genes. A mutation within the stem-loop sequence reduces the rate of frameshifting by about 80%. Artificial transposons carrying this mutation transpose at a normal frequency, but form cointegrates at a approximately 100-fold reduced rate. |
format | Text |
id | pubmed-328623 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1991 |
record_format | MEDLINE/PubMed |
spelling | pubmed-3286232004-02-10 High-level ribosomal frameshifting directs the synthesis of IS150 gene products. Vögele, K Schwartz, E Welz, C Schiltz, E Rak, B Nucleic Acids Res IS150 contains two tandem, out-of-phase, overlapping genes, ins150A and ins150B, which are controlled by the same promoter. These genes encode proteins of 19 and 31 kD, respectively. A third protein of 49 kD is a transframe gene product consisting of domains encoded by both genes. Specific -1 ribosomal frameshifting is responsible for the synthesis of the large protein. Expression of ins150B also involves frameshifting. The IS150 frameshifting signals operate with a remarkably high efficiency, causing about one third of the ribosomes to switch frame. All of the signals required for this process are encoded in a 83-bp segment of the element. The heptanucleotide A AAA AAG and a potential stem-loop-forming sequence mark the frameshifting site. Similar sequence elements are found in -1 frameshifting regions of bacterial and retroviral genes. A mutation within the stem-loop sequence reduces the rate of frameshifting by about 80%. Artificial transposons carrying this mutation transpose at a normal frequency, but form cointegrates at a approximately 100-fold reduced rate. 1991-08-25 /pmc/articles/PMC328623/ /pubmed/1653413 Text en |
spellingShingle | Vögele, K Schwartz, E Welz, C Schiltz, E Rak, B High-level ribosomal frameshifting directs the synthesis of IS150 gene products. |
title | High-level ribosomal frameshifting directs the synthesis of IS150 gene products. |
title_full | High-level ribosomal frameshifting directs the synthesis of IS150 gene products. |
title_fullStr | High-level ribosomal frameshifting directs the synthesis of IS150 gene products. |
title_full_unstemmed | High-level ribosomal frameshifting directs the synthesis of IS150 gene products. |
title_short | High-level ribosomal frameshifting directs the synthesis of IS150 gene products. |
title_sort | high-level ribosomal frameshifting directs the synthesis of is150 gene products. |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC328623/ https://www.ncbi.nlm.nih.gov/pubmed/1653413 |
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