Cargando…

Effect of Turkish propolis extracts on proteome of prostate cancer cell line

BACKGROUND: Propolis is a natural, resinous hive product that has several pharmacological activities. Its composition varies depending on the vegetation, climate, season and environmental conditions of the area from where it was collected. Surface enhanced laser desorption ionization time of flight...

Descripción completa

Detalles Bibliográficos
Autores principales: Barlak, Yaşam, Değer, Orhan, Çolak, Meltem, Karataylı, Senem Ceren, Bozdayı, Abdurrahman Mithat, Yücesan, Fulya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286392/
https://www.ncbi.nlm.nih.gov/pubmed/22152088
http://dx.doi.org/10.1186/1477-5956-9-74
_version_ 1782224547596992512
author Barlak, Yaşam
Değer, Orhan
Çolak, Meltem
Karataylı, Senem Ceren
Bozdayı, Abdurrahman Mithat
Yücesan, Fulya
author_facet Barlak, Yaşam
Değer, Orhan
Çolak, Meltem
Karataylı, Senem Ceren
Bozdayı, Abdurrahman Mithat
Yücesan, Fulya
author_sort Barlak, Yaşam
collection PubMed
description BACKGROUND: Propolis is a natural, resinous hive product that has several pharmacological activities. Its composition varies depending on the vegetation, climate, season and environmental conditions of the area from where it was collected. Surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF MS) is a proteomic approach which has been used in cancer proteomics studies. Prostate cancer is one of the most commonly diagnosed cancers in men. It has shown that nutritional supplements rich in polyphenolic compounds such as propolis play a significant role in prostate cancer chemoprevention. The aim of this study is to evaluate if protein expression profile in PC-3 prostate cancer cell lines could be differentiated when incubated with dimethyl sulfoxide and water extracts of Turkish propolis. RESULTS: The antioxidant potentials of dimethyl sulfoxide and water extracts of propolis were found in correlation with the amount of total phenolic compounds of them. Dimethyl sulfoxide and water extracts of propolis of 20 μg/mL reduced the cell viability to 24.5% and 17.7%, respectively. Statistically significant discriminatory peaks between control PC-3 cells and dimethyl sulfoxide extract of propolis-treated PC-3 cells were found to be the proteomic features at m/z 5143, 8703, 12661, 20184 and 32794, detected by CM10 ProteinChip, and the peak at m/z 3772, detected by Q10 ProteinChip. Between control PC-3 cells and water extract of propolis-treated PC-3 cells, statistically significant discriminatory peaks were found to be the proteomic features at m/z 15846, 16052 and 24658, detected by CM10 ProteinChip and the peaks at m/z 10348, 10899 and 11603, detected by Q10 ProteinChip. CONCLUSIONS: It was concluded that dimethyl sulfoxide and water extracts of Turkish propolis may have anti-proliferative activity through differentiating protein expression profile in PC-3 prostate cancer cell lines along with their antioxidant capacity.
format Online
Article
Text
id pubmed-3286392
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-32863922012-02-25 Effect of Turkish propolis extracts on proteome of prostate cancer cell line Barlak, Yaşam Değer, Orhan Çolak, Meltem Karataylı, Senem Ceren Bozdayı, Abdurrahman Mithat Yücesan, Fulya Proteome Sci Research BACKGROUND: Propolis is a natural, resinous hive product that has several pharmacological activities. Its composition varies depending on the vegetation, climate, season and environmental conditions of the area from where it was collected. Surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF MS) is a proteomic approach which has been used in cancer proteomics studies. Prostate cancer is one of the most commonly diagnosed cancers in men. It has shown that nutritional supplements rich in polyphenolic compounds such as propolis play a significant role in prostate cancer chemoprevention. The aim of this study is to evaluate if protein expression profile in PC-3 prostate cancer cell lines could be differentiated when incubated with dimethyl sulfoxide and water extracts of Turkish propolis. RESULTS: The antioxidant potentials of dimethyl sulfoxide and water extracts of propolis were found in correlation with the amount of total phenolic compounds of them. Dimethyl sulfoxide and water extracts of propolis of 20 μg/mL reduced the cell viability to 24.5% and 17.7%, respectively. Statistically significant discriminatory peaks between control PC-3 cells and dimethyl sulfoxide extract of propolis-treated PC-3 cells were found to be the proteomic features at m/z 5143, 8703, 12661, 20184 and 32794, detected by CM10 ProteinChip, and the peak at m/z 3772, detected by Q10 ProteinChip. Between control PC-3 cells and water extract of propolis-treated PC-3 cells, statistically significant discriminatory peaks were found to be the proteomic features at m/z 15846, 16052 and 24658, detected by CM10 ProteinChip and the peaks at m/z 10348, 10899 and 11603, detected by Q10 ProteinChip. CONCLUSIONS: It was concluded that dimethyl sulfoxide and water extracts of Turkish propolis may have anti-proliferative activity through differentiating protein expression profile in PC-3 prostate cancer cell lines along with their antioxidant capacity. BioMed Central 2011-12-07 /pmc/articles/PMC3286392/ /pubmed/22152088 http://dx.doi.org/10.1186/1477-5956-9-74 Text en Copyright ©2011 Barlak et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Barlak, Yaşam
Değer, Orhan
Çolak, Meltem
Karataylı, Senem Ceren
Bozdayı, Abdurrahman Mithat
Yücesan, Fulya
Effect of Turkish propolis extracts on proteome of prostate cancer cell line
title Effect of Turkish propolis extracts on proteome of prostate cancer cell line
title_full Effect of Turkish propolis extracts on proteome of prostate cancer cell line
title_fullStr Effect of Turkish propolis extracts on proteome of prostate cancer cell line
title_full_unstemmed Effect of Turkish propolis extracts on proteome of prostate cancer cell line
title_short Effect of Turkish propolis extracts on proteome of prostate cancer cell line
title_sort effect of turkish propolis extracts on proteome of prostate cancer cell line
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286392/
https://www.ncbi.nlm.nih.gov/pubmed/22152088
http://dx.doi.org/10.1186/1477-5956-9-74
work_keys_str_mv AT barlakyasam effectofturkishpropolisextractsonproteomeofprostatecancercellline
AT degerorhan effectofturkishpropolisextractsonproteomeofprostatecancercellline
AT colakmeltem effectofturkishpropolisextractsonproteomeofprostatecancercellline
AT karataylısenemceren effectofturkishpropolisextractsonproteomeofprostatecancercellline
AT bozdayıabdurrahmanmithat effectofturkishpropolisextractsonproteomeofprostatecancercellline
AT yucesanfulya effectofturkishpropolisextractsonproteomeofprostatecancercellline