The order of expression is a key factor in the production of active transglutaminase in Escherichia coli by co-expression with its pro-peptide

BACKGROUND: Streptomyces transglutaminase (TGase) is naturally synthesized as zymogen (pro-TGase), which is then processed to produce active enzyme by the removal of its N-terminal pro-peptide. This pro-peptide is found to be essential for overexpression of soluble TGase in E. coli. However, express...

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Detalles Bibliográficos
Autores principales: Liu, Song, Zhang, Dongxu, Wang, Miao, Cui, Wenjing, Chen, Kangkang, Du, Guocheng, Chen, Jian, Zhou, Zhemin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286405/
https://www.ncbi.nlm.nih.gov/pubmed/22196373
http://dx.doi.org/10.1186/1475-2859-10-112

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