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Comparison of Functional Proteomic Analyses of Human Breast Cancer Cell Lines T47D and MCF7

T47D and MCF7 are two human hormone-dependent breast cancer cell lines which are widely used as experimental models for in vitro and in vivo (tumor xenografts) breast cancer studies. Several proteins involved in cancer development were identified in these cell lines by proteomic analyses. Although t...

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Autores principales: Adjo Aka, Juliette, Lin, Sheng-Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286449/
https://www.ncbi.nlm.nih.gov/pubmed/22384035
http://dx.doi.org/10.1371/journal.pone.0031532
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author Adjo Aka, Juliette
Lin, Sheng-Xiang
author_facet Adjo Aka, Juliette
Lin, Sheng-Xiang
author_sort Adjo Aka, Juliette
collection PubMed
description T47D and MCF7 are two human hormone-dependent breast cancer cell lines which are widely used as experimental models for in vitro and in vivo (tumor xenografts) breast cancer studies. Several proteins involved in cancer development were identified in these cell lines by proteomic analyses. Although these studies reported the proteomic profiles of each cell line, until now, their differential protein expression profiles have not been established. Here, we used two-dimensional gel and mass spectrometry analyses to compare the proteomic profiles of the two cell lines, T47D and MCF7. Our data revealed that more than 164 proteins are differentially expressed between them. According to their biological functions, the results showed that proteins involved in cell growth stimulation, anti-apoptosis mechanisms and cancerogenesis are more strongly expressed in T47D than in MCF7. These proteins include G1/S-specific cyclin-D3 and prohibitin. Proteins implicated in transcription repression and apoptosis regulation, including transcriptional repressor NF-X1, nitrilase homolog 2 and interleukin-10, are, on the contrary, more strongly expressed in MCF7 as compared to T47D. Five proteins that were previously described as breast cancer biomarkers, namely cathepsin D, cathepsin B, protein S100-A14, heat shock protein beta-1 (HSP27) and proliferating cell nuclear antigen (PCNA), are found to be differentially expressed in the two cell lines. A list of differentially expressed proteins between T47D and MCF7 was generated, providing useful information for further studies of breast cancer mechanisms with these cell lines as models.
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spelling pubmed-32864492012-03-01 Comparison of Functional Proteomic Analyses of Human Breast Cancer Cell Lines T47D and MCF7 Adjo Aka, Juliette Lin, Sheng-Xiang PLoS One Research Article T47D and MCF7 are two human hormone-dependent breast cancer cell lines which are widely used as experimental models for in vitro and in vivo (tumor xenografts) breast cancer studies. Several proteins involved in cancer development were identified in these cell lines by proteomic analyses. Although these studies reported the proteomic profiles of each cell line, until now, their differential protein expression profiles have not been established. Here, we used two-dimensional gel and mass spectrometry analyses to compare the proteomic profiles of the two cell lines, T47D and MCF7. Our data revealed that more than 164 proteins are differentially expressed between them. According to their biological functions, the results showed that proteins involved in cell growth stimulation, anti-apoptosis mechanisms and cancerogenesis are more strongly expressed in T47D than in MCF7. These proteins include G1/S-specific cyclin-D3 and prohibitin. Proteins implicated in transcription repression and apoptosis regulation, including transcriptional repressor NF-X1, nitrilase homolog 2 and interleukin-10, are, on the contrary, more strongly expressed in MCF7 as compared to T47D. Five proteins that were previously described as breast cancer biomarkers, namely cathepsin D, cathepsin B, protein S100-A14, heat shock protein beta-1 (HSP27) and proliferating cell nuclear antigen (PCNA), are found to be differentially expressed in the two cell lines. A list of differentially expressed proteins between T47D and MCF7 was generated, providing useful information for further studies of breast cancer mechanisms with these cell lines as models. Public Library of Science 2012-02-24 /pmc/articles/PMC3286449/ /pubmed/22384035 http://dx.doi.org/10.1371/journal.pone.0031532 Text en Adjo Aka, Lin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Adjo Aka, Juliette
Lin, Sheng-Xiang
Comparison of Functional Proteomic Analyses of Human Breast Cancer Cell Lines T47D and MCF7
title Comparison of Functional Proteomic Analyses of Human Breast Cancer Cell Lines T47D and MCF7
title_full Comparison of Functional Proteomic Analyses of Human Breast Cancer Cell Lines T47D and MCF7
title_fullStr Comparison of Functional Proteomic Analyses of Human Breast Cancer Cell Lines T47D and MCF7
title_full_unstemmed Comparison of Functional Proteomic Analyses of Human Breast Cancer Cell Lines T47D and MCF7
title_short Comparison of Functional Proteomic Analyses of Human Breast Cancer Cell Lines T47D and MCF7
title_sort comparison of functional proteomic analyses of human breast cancer cell lines t47d and mcf7
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286449/
https://www.ncbi.nlm.nih.gov/pubmed/22384035
http://dx.doi.org/10.1371/journal.pone.0031532
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