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Disentangling the Intertwined Genetic Bases of Root and Shoot Growth in Arabidopsis

Root growth and architecture are major components of plant nutrient and water use efficiencies and these traits are the matter of extensive genetic analysis in several crop species. Because root growth relies on exported assimilate from the shoot, and changes in assimilate supply are known to alter...

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Autores principales: Bouteillé, Marie, Rolland, Gaëlle, Balsera, Crispulo, Loudet, Olivier, Muller, Bertrand
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286473/
https://www.ncbi.nlm.nih.gov/pubmed/22384215
http://dx.doi.org/10.1371/journal.pone.0032319
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author Bouteillé, Marie
Rolland, Gaëlle
Balsera, Crispulo
Loudet, Olivier
Muller, Bertrand
author_facet Bouteillé, Marie
Rolland, Gaëlle
Balsera, Crispulo
Loudet, Olivier
Muller, Bertrand
author_sort Bouteillé, Marie
collection PubMed
description Root growth and architecture are major components of plant nutrient and water use efficiencies and these traits are the matter of extensive genetic analysis in several crop species. Because root growth relies on exported assimilate from the shoot, and changes in assimilate supply are known to alter root architecture, we hypothesized (i) that the genetic bases of root growth could be intertwined with the genetic bases of shoot growth and (ii) that the link could be either positive, with alleles favouring shoot growth also favouring root growth, or negative, because of competition for assimilates. We tested these hypotheses using a quantitative genetics approach in the model species Arabidopsis thaliana and the Bay-0×Shahdara recombinant inbred lines population. In accordance with our hypothesis, root and shoot growth traits were strongly correlated and most root growth quantitative trait loci (QTLs) colocalized with shoot growth QTLs with positive alleles originating from either the same or the opposite parent. In order to identify regions that could be responsible for root growth independently of the shoot, we generated new variables either based on root to shoot ratios, residuals of root to shoot correlations or coordinates of principal component analysis. These variables showed high heritability allowing genetic analysis. They essentially all yielded similar results pointing towards two regions involved in the root – shoot balance. Using Heterogeneous Inbred Families (a kind of near-isogenic lines), we validated part of the QTLs present in these two regions for different traits. Our study thus highlights the difficulty of disentangling intertwined genetic bases of root and shoot growth and shows that this difficulty can be overcome by using simple statistical tools.
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spelling pubmed-32864732012-03-01 Disentangling the Intertwined Genetic Bases of Root and Shoot Growth in Arabidopsis Bouteillé, Marie Rolland, Gaëlle Balsera, Crispulo Loudet, Olivier Muller, Bertrand PLoS One Research Article Root growth and architecture are major components of plant nutrient and water use efficiencies and these traits are the matter of extensive genetic analysis in several crop species. Because root growth relies on exported assimilate from the shoot, and changes in assimilate supply are known to alter root architecture, we hypothesized (i) that the genetic bases of root growth could be intertwined with the genetic bases of shoot growth and (ii) that the link could be either positive, with alleles favouring shoot growth also favouring root growth, or negative, because of competition for assimilates. We tested these hypotheses using a quantitative genetics approach in the model species Arabidopsis thaliana and the Bay-0×Shahdara recombinant inbred lines population. In accordance with our hypothesis, root and shoot growth traits were strongly correlated and most root growth quantitative trait loci (QTLs) colocalized with shoot growth QTLs with positive alleles originating from either the same or the opposite parent. In order to identify regions that could be responsible for root growth independently of the shoot, we generated new variables either based on root to shoot ratios, residuals of root to shoot correlations or coordinates of principal component analysis. These variables showed high heritability allowing genetic analysis. They essentially all yielded similar results pointing towards two regions involved in the root – shoot balance. Using Heterogeneous Inbred Families (a kind of near-isogenic lines), we validated part of the QTLs present in these two regions for different traits. Our study thus highlights the difficulty of disentangling intertwined genetic bases of root and shoot growth and shows that this difficulty can be overcome by using simple statistical tools. Public Library of Science 2012-02-24 /pmc/articles/PMC3286473/ /pubmed/22384215 http://dx.doi.org/10.1371/journal.pone.0032319 Text en Bouteillé et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bouteillé, Marie
Rolland, Gaëlle
Balsera, Crispulo
Loudet, Olivier
Muller, Bertrand
Disentangling the Intertwined Genetic Bases of Root and Shoot Growth in Arabidopsis
title Disentangling the Intertwined Genetic Bases of Root and Shoot Growth in Arabidopsis
title_full Disentangling the Intertwined Genetic Bases of Root and Shoot Growth in Arabidopsis
title_fullStr Disentangling the Intertwined Genetic Bases of Root and Shoot Growth in Arabidopsis
title_full_unstemmed Disentangling the Intertwined Genetic Bases of Root and Shoot Growth in Arabidopsis
title_short Disentangling the Intertwined Genetic Bases of Root and Shoot Growth in Arabidopsis
title_sort disentangling the intertwined genetic bases of root and shoot growth in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286473/
https://www.ncbi.nlm.nih.gov/pubmed/22384215
http://dx.doi.org/10.1371/journal.pone.0032319
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