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TLR4 dependent heparan sulphate-induced pancreatic inflammatory response is IRF3-mediated

BACKGROUND: Degraded extracellular matrix can stimulate the innate immune system via the Toll-Like Receptor-4 (TLR4). In the pancreas, syndecan-anchored heparan sulphate (HS) on the ductal epithelium can be cleaved off its protein cores by the proteases (trypsin and elastase) and potentially activat...

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Autores principales: Akbarshahi, Hamid, Axelsson, Jakob BF, Said, Katarzyna, Malmström, Anders, Fischer, Hans, Andersson, Roland
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286488/
https://www.ncbi.nlm.nih.gov/pubmed/22188870
http://dx.doi.org/10.1186/1479-5876-9-219
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author Akbarshahi, Hamid
Axelsson, Jakob BF
Said, Katarzyna
Malmström, Anders
Fischer, Hans
Andersson, Roland
author_facet Akbarshahi, Hamid
Axelsson, Jakob BF
Said, Katarzyna
Malmström, Anders
Fischer, Hans
Andersson, Roland
author_sort Akbarshahi, Hamid
collection PubMed
description BACKGROUND: Degraded extracellular matrix can stimulate the innate immune system via the Toll-Like Receptor-4 (TLR4). In the pancreas, syndecan-anchored heparan sulphate (HS) on the ductal epithelium can be cleaved off its protein cores by the proteases (trypsin and elastase) and potentially activate TLR4 signalling. METHODS: To investigate this signalling event, a low sulphated HS (500 μg/ml) was infused into the biliary-pancreatic duct of C57BL/6J wild-type mice. Phosphate buffered saline (PBS) and lipopolysaccharide (LPS) were used as negative and positive controls, respectively. Mice were sacrificed after 1, 3, 6, 9, and 48 hours and tissues were analysed for neutrophil and cytokine contents. In order to study the TLR4 signalling pathway of HS in the pancreas, genetically engineered mice lacking TLR4, Myeloid Differentiation primary response gene (88) (MyD88) or Interferon Regulatory Factor 3 (IRF3) were subjected to pancreatic infusion of HS. RESULTS: Neutrophil sequestration and corresponding myeloperoxidase (MPO) activity in the pancreas were increased 9 hours following HS challenge. In wild-type mice, the monocyte chemoattractant protein-1(MCP-1) increased at 3 hours after infusion, while RANTES increased after 9 hours. TLR4, MyD88, and IRF3 knockout mice showed an abrogated neutrophil recruitment and myeloperoxidase activity in the HS group, while the LPS response was only abolished in TLR4 and MyD88 knockouts. CONCLUSIONS: The results of this study show that HS is capable of initiating a TLR4-dependent innate immune response in the pancreas which is distinctly different from that induced by LPS. This inflammatory response was mediated predominantly through IRF3- dependent pathway. Release of HS into the pancreatic duct may be one important mediator in the pancreatic ductal defence.
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spelling pubmed-32864882012-02-26 TLR4 dependent heparan sulphate-induced pancreatic inflammatory response is IRF3-mediated Akbarshahi, Hamid Axelsson, Jakob BF Said, Katarzyna Malmström, Anders Fischer, Hans Andersson, Roland J Transl Med Research BACKGROUND: Degraded extracellular matrix can stimulate the innate immune system via the Toll-Like Receptor-4 (TLR4). In the pancreas, syndecan-anchored heparan sulphate (HS) on the ductal epithelium can be cleaved off its protein cores by the proteases (trypsin and elastase) and potentially activate TLR4 signalling. METHODS: To investigate this signalling event, a low sulphated HS (500 μg/ml) was infused into the biliary-pancreatic duct of C57BL/6J wild-type mice. Phosphate buffered saline (PBS) and lipopolysaccharide (LPS) were used as negative and positive controls, respectively. Mice were sacrificed after 1, 3, 6, 9, and 48 hours and tissues were analysed for neutrophil and cytokine contents. In order to study the TLR4 signalling pathway of HS in the pancreas, genetically engineered mice lacking TLR4, Myeloid Differentiation primary response gene (88) (MyD88) or Interferon Regulatory Factor 3 (IRF3) were subjected to pancreatic infusion of HS. RESULTS: Neutrophil sequestration and corresponding myeloperoxidase (MPO) activity in the pancreas were increased 9 hours following HS challenge. In wild-type mice, the monocyte chemoattractant protein-1(MCP-1) increased at 3 hours after infusion, while RANTES increased after 9 hours. TLR4, MyD88, and IRF3 knockout mice showed an abrogated neutrophil recruitment and myeloperoxidase activity in the HS group, while the LPS response was only abolished in TLR4 and MyD88 knockouts. CONCLUSIONS: The results of this study show that HS is capable of initiating a TLR4-dependent innate immune response in the pancreas which is distinctly different from that induced by LPS. This inflammatory response was mediated predominantly through IRF3- dependent pathway. Release of HS into the pancreatic duct may be one important mediator in the pancreatic ductal defence. BioMed Central 2011-12-21 /pmc/articles/PMC3286488/ /pubmed/22188870 http://dx.doi.org/10.1186/1479-5876-9-219 Text en Copyright ©2011 Akbarshahi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Akbarshahi, Hamid
Axelsson, Jakob BF
Said, Katarzyna
Malmström, Anders
Fischer, Hans
Andersson, Roland
TLR4 dependent heparan sulphate-induced pancreatic inflammatory response is IRF3-mediated
title TLR4 dependent heparan sulphate-induced pancreatic inflammatory response is IRF3-mediated
title_full TLR4 dependent heparan sulphate-induced pancreatic inflammatory response is IRF3-mediated
title_fullStr TLR4 dependent heparan sulphate-induced pancreatic inflammatory response is IRF3-mediated
title_full_unstemmed TLR4 dependent heparan sulphate-induced pancreatic inflammatory response is IRF3-mediated
title_short TLR4 dependent heparan sulphate-induced pancreatic inflammatory response is IRF3-mediated
title_sort tlr4 dependent heparan sulphate-induced pancreatic inflammatory response is irf3-mediated
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286488/
https://www.ncbi.nlm.nih.gov/pubmed/22188870
http://dx.doi.org/10.1186/1479-5876-9-219
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