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Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum
We compared biological functions of two acetylcholinesterase genes (TcAce1 and TcAce2) in Tribolium castaneum, a globally distributed major pest of stored grain products and an emerging model organism, by using RNA interference. Although both genes expressed at all developmental stages and mainly in...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286809/ https://www.ncbi.nlm.nih.gov/pubmed/22371826 http://dx.doi.org/10.1038/srep00288 |
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author | Lu, Yanhui Park, Yoonseong Gao, Xiwu Zhang, Xin Yao, Jianxiu Pang, Yuan-Ping Jiang, Haobo Zhu, Kun Yan |
author_facet | Lu, Yanhui Park, Yoonseong Gao, Xiwu Zhang, Xin Yao, Jianxiu Pang, Yuan-Ping Jiang, Haobo Zhu, Kun Yan |
author_sort | Lu, Yanhui |
collection | PubMed |
description | We compared biological functions of two acetylcholinesterase genes (TcAce1 and TcAce2) in Tribolium castaneum, a globally distributed major pest of stored grain products and an emerging model organism, by using RNA interference. Although both genes expressed at all developmental stages and mainly in the brain, the transcript level of TcAce1 was 1.2- to 8.7-fold higher than that of TcAce2, depending on developmental stages. Silencing TcAce1 in 20-day larvae led to 100% mortality within two weeks after eclosion and increased larval susceptibilities to anticholinesterase insecticides. In contrast, silencing TcAce2 did not show insect mortality and significantly affect insecticide susceptibility, but delayed insect development and reduced female egg-laying and egg hatching. These results demonstrate for the first time that TcAce1 plays a major role in cholinergic functions and is the target of anticholinesterase insecticides, whereas TcAce2 plays an important, non-cholinergic role in female reproduction, embryo development, and growth of offspring. |
format | Online Article Text |
id | pubmed-3286809 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-32868092012-02-27 Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum Lu, Yanhui Park, Yoonseong Gao, Xiwu Zhang, Xin Yao, Jianxiu Pang, Yuan-Ping Jiang, Haobo Zhu, Kun Yan Sci Rep Article We compared biological functions of two acetylcholinesterase genes (TcAce1 and TcAce2) in Tribolium castaneum, a globally distributed major pest of stored grain products and an emerging model organism, by using RNA interference. Although both genes expressed at all developmental stages and mainly in the brain, the transcript level of TcAce1 was 1.2- to 8.7-fold higher than that of TcAce2, depending on developmental stages. Silencing TcAce1 in 20-day larvae led to 100% mortality within two weeks after eclosion and increased larval susceptibilities to anticholinesterase insecticides. In contrast, silencing TcAce2 did not show insect mortality and significantly affect insecticide susceptibility, but delayed insect development and reduced female egg-laying and egg hatching. These results demonstrate for the first time that TcAce1 plays a major role in cholinergic functions and is the target of anticholinesterase insecticides, whereas TcAce2 plays an important, non-cholinergic role in female reproduction, embryo development, and growth of offspring. Nature Publishing Group 2012-02-27 /pmc/articles/PMC3286809/ /pubmed/22371826 http://dx.doi.org/10.1038/srep00288 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
spellingShingle | Article Lu, Yanhui Park, Yoonseong Gao, Xiwu Zhang, Xin Yao, Jianxiu Pang, Yuan-Ping Jiang, Haobo Zhu, Kun Yan Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum |
title | Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum |
title_full | Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum |
title_fullStr | Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum |
title_full_unstemmed | Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum |
title_short | Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum |
title_sort | cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in tribolium castaneum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286809/ https://www.ncbi.nlm.nih.gov/pubmed/22371826 http://dx.doi.org/10.1038/srep00288 |
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