Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae

We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto sol...

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Detalles Bibliográficos
Autores principales: Pereira, Hugo, Barreira, Luísa, Mozes, André, Florindo, Cláudia, Polo, Cristina, Duarte, Catarina V, Custódio, Luísa, Varela, João
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287250/
https://www.ncbi.nlm.nih.gov/pubmed/22192119
http://dx.doi.org/10.1186/1754-6834-4-61
Descripción
Sumario:We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.

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