Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae

We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto sol...

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Detalles Bibliográficos
Autores principales: Pereira, Hugo, Barreira, Luísa, Mozes, André, Florindo, Cláudia, Polo, Cristina, Duarte, Catarina V, Custódio, Luísa, Varela, João
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287250/
https://www.ncbi.nlm.nih.gov/pubmed/22192119
http://dx.doi.org/10.1186/1754-6834-4-61
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author Pereira, Hugo
Barreira, Luísa
Mozes, André
Florindo, Cláudia
Polo, Cristina
Duarte, Catarina V
Custódio, Luísa
Varela, João
author_facet Pereira, Hugo
Barreira, Luísa
Mozes, André
Florindo, Cláudia
Polo, Cristina
Duarte, Catarina V
Custódio, Luísa
Varela, João
author_sort Pereira, Hugo
collection PubMed
description We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.
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spelling pubmed-32872502012-02-28 Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae Pereira, Hugo Barreira, Luísa Mozes, André Florindo, Cláudia Polo, Cristina Duarte, Catarina V Custódio, Luísa Varela, João Biotechnol Biofuels Methodology We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla. BioMed Central 2011-12-22 /pmc/articles/PMC3287250/ /pubmed/22192119 http://dx.doi.org/10.1186/1754-6834-4-61 Text en Copyright ©2011 Pereira et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Pereira, Hugo
Barreira, Luísa
Mozes, André
Florindo, Cláudia
Polo, Cristina
Duarte, Catarina V
Custódio, Luísa
Varela, João
Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
title Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
title_full Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
title_fullStr Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
title_full_unstemmed Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
title_short Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
title_sort microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287250/
https://www.ncbi.nlm.nih.gov/pubmed/22192119
http://dx.doi.org/10.1186/1754-6834-4-61
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