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Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae)

BACKGROUND: The predatory mirids of the genus Macrolophus are key natural enemies of various economically important agricultural pests. Both M. caliginosus and M. pygmaeus are commercially available for the augmentative biological control of arthropod pests in European greenhouses. The latter specie...

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Autores principales: Machtelinckx, Thijs, Van Leeuwen, Thomas, Van De Wiele, Tom, Boon, Nico, De Vos, Winnok H, Sanchez, Juan-Antonio, Nannini, Mauro, Gheysen, Godelieve, De Clercq, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287520/
https://www.ncbi.nlm.nih.gov/pubmed/22376198
http://dx.doi.org/10.1186/1471-2180-12-S1-S9
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author Machtelinckx, Thijs
Van Leeuwen, Thomas
Van De Wiele, Tom
Boon, Nico
De Vos, Winnok H
Sanchez, Juan-Antonio
Nannini, Mauro
Gheysen, Godelieve
De Clercq, Patrick
author_facet Machtelinckx, Thijs
Van Leeuwen, Thomas
Van De Wiele, Tom
Boon, Nico
De Vos, Winnok H
Sanchez, Juan-Antonio
Nannini, Mauro
Gheysen, Godelieve
De Clercq, Patrick
author_sort Machtelinckx, Thijs
collection PubMed
description BACKGROUND: The predatory mirids of the genus Macrolophus are key natural enemies of various economically important agricultural pests. Both M. caliginosus and M. pygmaeus are commercially available for the augmentative biological control of arthropod pests in European greenhouses. The latter species is known to be infected with Wolbachia -inducing cytoplasmic incompatibility in its host- but the presence of other endosymbionts has not been demonstrated. In the present study, the microbial diversity was examined in various populations of M. caliginosus and M. pygmaeus by 16S rRNA sequencing and denaturing gradient gel electrophoresis. RESULTS: Besides Wolbachia, a co-infection of 2 Rickettsia species was detected in all M. pygmaeus populations. Based on a concatenated alignment of the 16S rRNA gene, the gltA gene and the coxA gene, the first is phylogenetically related to Rickettsia bellii, whereas the other is closely related to Rickettsia limoniae. All M. caliginosus populations were infected with the same Wolbachia and limoniae-like Rickettsia strain as M. pygmaeus, but did not harbour the bellii-like Rickettsia strain. Interestingly, individuals with a single infection were not found. A PCR assay on the ovaries of M. pygmaeus and M. caliginosus indicated that all endosymbionts are vertically transmitted. The presence of Wolbachia and Rickettsia in oocytes was confirmed by a fluorescence in situ hybridisation. A bio-assay comparing an infected and an uninfected M. pygmaeus population suggested that the endosymbionts had minor effects on nymphal development of their insect host and did not influence its fecundity. CONCLUSION: Two species of the palaearctic mirid genus Macrolophus are infected with multiple endosymbionts, including Wolbachia and Rickettsia. Independent of the origin, all tested populations of both M. pygmaeus and M. caliginosus were infected with three and two endosymbionts, respectively. There was no indication that infection with endosymbiotic bacteria had a fitness cost in terms of development and fecundity of the predators.
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spelling pubmed-32875202012-02-28 Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae) Machtelinckx, Thijs Van Leeuwen, Thomas Van De Wiele, Tom Boon, Nico De Vos, Winnok H Sanchez, Juan-Antonio Nannini, Mauro Gheysen, Godelieve De Clercq, Patrick BMC Microbiol Research BACKGROUND: The predatory mirids of the genus Macrolophus are key natural enemies of various economically important agricultural pests. Both M. caliginosus and M. pygmaeus are commercially available for the augmentative biological control of arthropod pests in European greenhouses. The latter species is known to be infected with Wolbachia -inducing cytoplasmic incompatibility in its host- but the presence of other endosymbionts has not been demonstrated. In the present study, the microbial diversity was examined in various populations of M. caliginosus and M. pygmaeus by 16S rRNA sequencing and denaturing gradient gel electrophoresis. RESULTS: Besides Wolbachia, a co-infection of 2 Rickettsia species was detected in all M. pygmaeus populations. Based on a concatenated alignment of the 16S rRNA gene, the gltA gene and the coxA gene, the first is phylogenetically related to Rickettsia bellii, whereas the other is closely related to Rickettsia limoniae. All M. caliginosus populations were infected with the same Wolbachia and limoniae-like Rickettsia strain as M. pygmaeus, but did not harbour the bellii-like Rickettsia strain. Interestingly, individuals with a single infection were not found. A PCR assay on the ovaries of M. pygmaeus and M. caliginosus indicated that all endosymbionts are vertically transmitted. The presence of Wolbachia and Rickettsia in oocytes was confirmed by a fluorescence in situ hybridisation. A bio-assay comparing an infected and an uninfected M. pygmaeus population suggested that the endosymbionts had minor effects on nymphal development of their insect host and did not influence its fecundity. CONCLUSION: Two species of the palaearctic mirid genus Macrolophus are infected with multiple endosymbionts, including Wolbachia and Rickettsia. Independent of the origin, all tested populations of both M. pygmaeus and M. caliginosus were infected with three and two endosymbionts, respectively. There was no indication that infection with endosymbiotic bacteria had a fitness cost in terms of development and fecundity of the predators. BioMed Central 2012-01-18 /pmc/articles/PMC3287520/ /pubmed/22376198 http://dx.doi.org/10.1186/1471-2180-12-S1-S9 Text en Copyright ©2012 Machtelinckx et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Machtelinckx, Thijs
Van Leeuwen, Thomas
Van De Wiele, Tom
Boon, Nico
De Vos, Winnok H
Sanchez, Juan-Antonio
Nannini, Mauro
Gheysen, Godelieve
De Clercq, Patrick
Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae)
title Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae)
title_full Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae)
title_fullStr Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae)
title_full_unstemmed Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae)
title_short Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae)
title_sort microbial community of predatory bugs of the genus macrolophus (hemiptera: miridae)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287520/
https://www.ncbi.nlm.nih.gov/pubmed/22376198
http://dx.doi.org/10.1186/1471-2180-12-S1-S9
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