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Impact of Chikungunya Virus on Aedes albopictus Females and Possibility of Vertical Transmission Using the Actors of the 2007 Outbreak in Italy

We investigated the impact of CHIKV strains on some Aedes albopictus (Skuse) reproductive parameters and the possibility of vertical transmission. Two strains were collected in the area where the epidemic occurred in 2007, one isolated from mosquitoes, the other one isolated from a viraemic patient....

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Autores principales: Bellini, Romeo, Medici, Anna, Calzolari, Mattia, Bonilauri, Paolo, Cavrini, Francesca, Sambri, Vittorio, Angelini, Paola, Dottori, Michele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287980/
https://www.ncbi.nlm.nih.gov/pubmed/22383945
http://dx.doi.org/10.1371/journal.pone.0028360
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author Bellini, Romeo
Medici, Anna
Calzolari, Mattia
Bonilauri, Paolo
Cavrini, Francesca
Sambri, Vittorio
Angelini, Paola
Dottori, Michele
author_facet Bellini, Romeo
Medici, Anna
Calzolari, Mattia
Bonilauri, Paolo
Cavrini, Francesca
Sambri, Vittorio
Angelini, Paola
Dottori, Michele
author_sort Bellini, Romeo
collection PubMed
description We investigated the impact of CHIKV strains on some Aedes albopictus (Skuse) reproductive parameters and the possibility of vertical transmission. Two strains were collected in the area where the epidemic occurred in 2007, one isolated from mosquitoes, the other one isolated from a viraemic patient. Different types of blood meals, either infected or non-infected, were offered to Ae. albopictus females, that were then analyzed at increasing time post infection. The virus titre, measured by two RT-PCR methods in the blood meals, influenced the rate of infection and the rate of dissemination of CHIKV in Ae. albopictus body. We found individual variability with respect to the infection/dissemination rates and their latency both considering the female's body and appendages. The hatching rate was significantly lower for the eggs laid by the infected females than for the control eggs, while the mortality during the larval development (from first instar larva to adult emergence) was similar among the progeny of infected and non-infected female groups. Our findings seem to support the hypothesis that the vertical transmission is a rare event under our conditions, and that a certain time period is required in order to get the ovarioles infected. Field observations conducted during the Spring 2008 showed no evidence of the presence of infected overwintering progeny produced by Ae. albopictus females infected during the 2007 outbreak.
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spelling pubmed-32879802012-03-01 Impact of Chikungunya Virus on Aedes albopictus Females and Possibility of Vertical Transmission Using the Actors of the 2007 Outbreak in Italy Bellini, Romeo Medici, Anna Calzolari, Mattia Bonilauri, Paolo Cavrini, Francesca Sambri, Vittorio Angelini, Paola Dottori, Michele PLoS One Research Article We investigated the impact of CHIKV strains on some Aedes albopictus (Skuse) reproductive parameters and the possibility of vertical transmission. Two strains were collected in the area where the epidemic occurred in 2007, one isolated from mosquitoes, the other one isolated from a viraemic patient. Different types of blood meals, either infected or non-infected, were offered to Ae. albopictus females, that were then analyzed at increasing time post infection. The virus titre, measured by two RT-PCR methods in the blood meals, influenced the rate of infection and the rate of dissemination of CHIKV in Ae. albopictus body. We found individual variability with respect to the infection/dissemination rates and their latency both considering the female's body and appendages. The hatching rate was significantly lower for the eggs laid by the infected females than for the control eggs, while the mortality during the larval development (from first instar larva to adult emergence) was similar among the progeny of infected and non-infected female groups. Our findings seem to support the hypothesis that the vertical transmission is a rare event under our conditions, and that a certain time period is required in order to get the ovarioles infected. Field observations conducted during the Spring 2008 showed no evidence of the presence of infected overwintering progeny produced by Ae. albopictus females infected during the 2007 outbreak. Public Library of Science 2012-02-27 /pmc/articles/PMC3287980/ /pubmed/22383945 http://dx.doi.org/10.1371/journal.pone.0028360 Text en Bellini et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bellini, Romeo
Medici, Anna
Calzolari, Mattia
Bonilauri, Paolo
Cavrini, Francesca
Sambri, Vittorio
Angelini, Paola
Dottori, Michele
Impact of Chikungunya Virus on Aedes albopictus Females and Possibility of Vertical Transmission Using the Actors of the 2007 Outbreak in Italy
title Impact of Chikungunya Virus on Aedes albopictus Females and Possibility of Vertical Transmission Using the Actors of the 2007 Outbreak in Italy
title_full Impact of Chikungunya Virus on Aedes albopictus Females and Possibility of Vertical Transmission Using the Actors of the 2007 Outbreak in Italy
title_fullStr Impact of Chikungunya Virus on Aedes albopictus Females and Possibility of Vertical Transmission Using the Actors of the 2007 Outbreak in Italy
title_full_unstemmed Impact of Chikungunya Virus on Aedes albopictus Females and Possibility of Vertical Transmission Using the Actors of the 2007 Outbreak in Italy
title_short Impact of Chikungunya Virus on Aedes albopictus Females and Possibility of Vertical Transmission Using the Actors of the 2007 Outbreak in Italy
title_sort impact of chikungunya virus on aedes albopictus females and possibility of vertical transmission using the actors of the 2007 outbreak in italy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287980/
https://www.ncbi.nlm.nih.gov/pubmed/22383945
http://dx.doi.org/10.1371/journal.pone.0028360
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