Cargando…
An essential role for the Id1/PI3K/Akt/NFkB/survivin signalling pathway in promoting the proliferation of endothelial progenitor cells in vitro
The enhancement of re-endothelialisation is a critical therapeutic option for repairing injured blood vessels. Endothelial progenitor cells (EPCs) are the major source of cells that participate in endothelium repair and contribute to re-endothelialisation by reducing neointima formation after vascul...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3289789/ https://www.ncbi.nlm.nih.gov/pubmed/22139302 http://dx.doi.org/10.1007/s11010-011-1166-x |
_version_ | 1782224902739197952 |
---|---|
author | Li, Wei Wang, Hang Kuang, Chun-yan Zhu, Jin-kun Yu, Yang Qin, Zhe-xue Liu, Jie Huang, Lan |
author_facet | Li, Wei Wang, Hang Kuang, Chun-yan Zhu, Jin-kun Yu, Yang Qin, Zhe-xue Liu, Jie Huang, Lan |
author_sort | Li, Wei |
collection | PubMed |
description | The enhancement of re-endothelialisation is a critical therapeutic option for repairing injured blood vessels. Endothelial progenitor cells (EPCs) are the major source of cells that participate in endothelium repair and contribute to re-endothelialisation by reducing neointima formation after vascular injury. The over-expression of the inhibitor of differentiation or DNA binding 1 (Id1) significantly improved EPC proliferation. This study aimed to investigate the effects of Id1 on the phosphatidylinositol-3-kinase (PI3K)/Akt/nuclear factor kappa B (NFκB)/survivin signalling pathway and its significance in promoting EPC proliferation in vitro. Spleen-derived EPCs were cultured as previously described. Id1 was presented at low levels in EPCs, and was rapidly up-regulated by stimulation with vascular endothelial growth factor. We demonstrated that transient transfection of Id1 into EPCs activated the PI3K/Akt/NFκB/survivin signalling pathway and promoted EPC proliferation. The proliferation of EPCs was extensively inhibited by silencing of endogenous Id1, and knockdown of Id1 expression led to suppression of PI3K/Akt/NFκB/survivin signalling pathway in EPCs. In addition, blockade by the PI3K-specific inhibitor LY294002, Akt inhibitor, the NFκB inhibitor BAY 11-7082, the survivin inhibitor Curcumin, or the survivin inhibitor YM155 reduced the effects of Id1 transfection. These results suggest that the Id1/PI3K/Akt/NFκB/survivin signalling pathway plays a critical role in EPC proliferation. The Id1/PI3K/Akt/NFκB/survivin signalling pathway may represent a novel therapeutic target in the prevention of restenosis after vascular injury. |
format | Online Article Text |
id | pubmed-3289789 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-32897892012-03-16 An essential role for the Id1/PI3K/Akt/NFkB/survivin signalling pathway in promoting the proliferation of endothelial progenitor cells in vitro Li, Wei Wang, Hang Kuang, Chun-yan Zhu, Jin-kun Yu, Yang Qin, Zhe-xue Liu, Jie Huang, Lan Mol Cell Biochem Article The enhancement of re-endothelialisation is a critical therapeutic option for repairing injured blood vessels. Endothelial progenitor cells (EPCs) are the major source of cells that participate in endothelium repair and contribute to re-endothelialisation by reducing neointima formation after vascular injury. The over-expression of the inhibitor of differentiation or DNA binding 1 (Id1) significantly improved EPC proliferation. This study aimed to investigate the effects of Id1 on the phosphatidylinositol-3-kinase (PI3K)/Akt/nuclear factor kappa B (NFκB)/survivin signalling pathway and its significance in promoting EPC proliferation in vitro. Spleen-derived EPCs were cultured as previously described. Id1 was presented at low levels in EPCs, and was rapidly up-regulated by stimulation with vascular endothelial growth factor. We demonstrated that transient transfection of Id1 into EPCs activated the PI3K/Akt/NFκB/survivin signalling pathway and promoted EPC proliferation. The proliferation of EPCs was extensively inhibited by silencing of endogenous Id1, and knockdown of Id1 expression led to suppression of PI3K/Akt/NFκB/survivin signalling pathway in EPCs. In addition, blockade by the PI3K-specific inhibitor LY294002, Akt inhibitor, the NFκB inhibitor BAY 11-7082, the survivin inhibitor Curcumin, or the survivin inhibitor YM155 reduced the effects of Id1 transfection. These results suggest that the Id1/PI3K/Akt/NFκB/survivin signalling pathway plays a critical role in EPC proliferation. The Id1/PI3K/Akt/NFκB/survivin signalling pathway may represent a novel therapeutic target in the prevention of restenosis after vascular injury. Springer US 2011-12-03 2012 /pmc/articles/PMC3289789/ /pubmed/22139302 http://dx.doi.org/10.1007/s11010-011-1166-x Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Li, Wei Wang, Hang Kuang, Chun-yan Zhu, Jin-kun Yu, Yang Qin, Zhe-xue Liu, Jie Huang, Lan An essential role for the Id1/PI3K/Akt/NFkB/survivin signalling pathway in promoting the proliferation of endothelial progenitor cells in vitro |
title | An essential role for the Id1/PI3K/Akt/NFkB/survivin signalling pathway in promoting the proliferation of endothelial progenitor cells in vitro |
title_full | An essential role for the Id1/PI3K/Akt/NFkB/survivin signalling pathway in promoting the proliferation of endothelial progenitor cells in vitro |
title_fullStr | An essential role for the Id1/PI3K/Akt/NFkB/survivin signalling pathway in promoting the proliferation of endothelial progenitor cells in vitro |
title_full_unstemmed | An essential role for the Id1/PI3K/Akt/NFkB/survivin signalling pathway in promoting the proliferation of endothelial progenitor cells in vitro |
title_short | An essential role for the Id1/PI3K/Akt/NFkB/survivin signalling pathway in promoting the proliferation of endothelial progenitor cells in vitro |
title_sort | essential role for the id1/pi3k/akt/nfkb/survivin signalling pathway in promoting the proliferation of endothelial progenitor cells in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3289789/ https://www.ncbi.nlm.nih.gov/pubmed/22139302 http://dx.doi.org/10.1007/s11010-011-1166-x |
work_keys_str_mv | AT liwei anessentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT wanghang anessentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT kuangchunyan anessentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT zhujinkun anessentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT yuyang anessentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT qinzhexue anessentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT liujie anessentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT huanglan anessentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT liwei essentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT wanghang essentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT kuangchunyan essentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT zhujinkun essentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT yuyang essentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT qinzhexue essentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT liujie essentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro AT huanglan essentialrolefortheid1pi3kaktnfkbsurvivinsignallingpathwayinpromotingtheproliferationofendothelialprogenitorcellsinvitro |