Malignant Ovarian Tumors with Induced Expression of Carbonyl Reductase Show Spontaneous Regression

BACKGROUND: The present study investigated tumor proliferation in a tumor model using murine ovarian cancer cells with increased carbonyl reductase (CR) expression. METHODS: CR cDNA was transfected into murine T-Ag-MOSE ovarian cancer cells by lipofection. CR-transfected cells (CR induction group) o...

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Autores principales: Wang, Hui, Yokoyama, Yoshihito, Tsuchida, Shigeki, Mizunuma, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290113/
https://www.ncbi.nlm.nih.gov/pubmed/22408375
http://dx.doi.org/10.4137/CMO.S9005
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author Wang, Hui
Yokoyama, Yoshihito
Tsuchida, Shigeki
Mizunuma, Hideki
author_facet Wang, Hui
Yokoyama, Yoshihito
Tsuchida, Shigeki
Mizunuma, Hideki
author_sort Wang, Hui
collection PubMed
description BACKGROUND: The present study investigated tumor proliferation in a tumor model using murine ovarian cancer cells with increased carbonyl reductase (CR) expression. METHODS: CR cDNA was transfected into murine T-Ag-MOSE ovarian cancer cells by lipofection. CR-transfected cells (CR induction group) or empty vector-treated cells (control group) were injected into the backs of 8-week-old nude mice at a concentration of 0.5 × 10(6) per 0.2 mL. Subsequent tumor proliferation in both groups was observed for 5 weeks. RESULTS: The control group showed an increase in tumor volume during the 5 weeks of observation. However, tumor volume in the CR induction group increased up to the second week but then decreased continuously until the fifth week of observation. The tumor growth curves for the two groups showed a significant difference (Mann-Whitney U test, P < 0.001). Histological and biochemical experiments were performed using tumor tissues isolated in the third week. Necrosis and inflammatory cell infiltration were noted for tumors in the CR induction group. Also, the number of apoptotic cells was significantly increased in the CR induction group compared with the control group (P < 0.001). Milk fat globule EGF factor 8, an “eat-me” signal for phagocytes such as macrophages, was expressed extensively in the tumor cytoplasm and interstitial cells of the CR induction group, and engulfment of apoptotic cells by macrophages was observed. Vascular endothelial growth factor expression in tumors was notably decreased in the CR induction group compared with the control group. CONCLUSION: Increased necrosis due to engulfing of apoptotic cells by phagocytes attracted by increased milk fat globule EGF factor 8 was considered to be the mechanism of spontaneous tumor regression in the CR induction group.
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spelling pubmed-32901132012-03-09 Malignant Ovarian Tumors with Induced Expression of Carbonyl Reductase Show Spontaneous Regression Wang, Hui Yokoyama, Yoshihito Tsuchida, Shigeki Mizunuma, Hideki Clin Med Insights Oncol Original Research BACKGROUND: The present study investigated tumor proliferation in a tumor model using murine ovarian cancer cells with increased carbonyl reductase (CR) expression. METHODS: CR cDNA was transfected into murine T-Ag-MOSE ovarian cancer cells by lipofection. CR-transfected cells (CR induction group) or empty vector-treated cells (control group) were injected into the backs of 8-week-old nude mice at a concentration of 0.5 × 10(6) per 0.2 mL. Subsequent tumor proliferation in both groups was observed for 5 weeks. RESULTS: The control group showed an increase in tumor volume during the 5 weeks of observation. However, tumor volume in the CR induction group increased up to the second week but then decreased continuously until the fifth week of observation. The tumor growth curves for the two groups showed a significant difference (Mann-Whitney U test, P < 0.001). Histological and biochemical experiments were performed using tumor tissues isolated in the third week. Necrosis and inflammatory cell infiltration were noted for tumors in the CR induction group. Also, the number of apoptotic cells was significantly increased in the CR induction group compared with the control group (P < 0.001). Milk fat globule EGF factor 8, an “eat-me” signal for phagocytes such as macrophages, was expressed extensively in the tumor cytoplasm and interstitial cells of the CR induction group, and engulfment of apoptotic cells by macrophages was observed. Vascular endothelial growth factor expression in tumors was notably decreased in the CR induction group compared with the control group. CONCLUSION: Increased necrosis due to engulfing of apoptotic cells by phagocytes attracted by increased milk fat globule EGF factor 8 was considered to be the mechanism of spontaneous tumor regression in the CR induction group. Libertas Academica 2012-02-09 /pmc/articles/PMC3290113/ /pubmed/22408375 http://dx.doi.org/10.4137/CMO.S9005 Text en © the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.
spellingShingle Original Research
Wang, Hui
Yokoyama, Yoshihito
Tsuchida, Shigeki
Mizunuma, Hideki
Malignant Ovarian Tumors with Induced Expression of Carbonyl Reductase Show Spontaneous Regression
title Malignant Ovarian Tumors with Induced Expression of Carbonyl Reductase Show Spontaneous Regression
title_full Malignant Ovarian Tumors with Induced Expression of Carbonyl Reductase Show Spontaneous Regression
title_fullStr Malignant Ovarian Tumors with Induced Expression of Carbonyl Reductase Show Spontaneous Regression
title_full_unstemmed Malignant Ovarian Tumors with Induced Expression of Carbonyl Reductase Show Spontaneous Regression
title_short Malignant Ovarian Tumors with Induced Expression of Carbonyl Reductase Show Spontaneous Regression
title_sort malignant ovarian tumors with induced expression of carbonyl reductase show spontaneous regression
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290113/
https://www.ncbi.nlm.nih.gov/pubmed/22408375
http://dx.doi.org/10.4137/CMO.S9005
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