Cargando…

Dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer

The ultimate goal of the study is to provide an imaging tool to detect the earliest signs of glaucoma before clinically visible damage occurs to the retinal nerve fiber layer (RNFL). Studies have shown that the optical reflectance of the damaged RNFL at short wavelength (<560nm) is reduced much m...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Xiangyang, Hu, Jianming, Knighton, Robert W., Huang, Xiang-Run, Puliafito, Carmen A., Jiao, Shuliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290519/
https://www.ncbi.nlm.nih.gov/pubmed/21996906
http://dx.doi.org/10.1364/OE.19.019653
_version_ 1782225008557293568
author Zhang, Xiangyang
Hu, Jianming
Knighton, Robert W.
Huang, Xiang-Run
Puliafito, Carmen A.
Jiao, Shuliang
author_facet Zhang, Xiangyang
Hu, Jianming
Knighton, Robert W.
Huang, Xiang-Run
Puliafito, Carmen A.
Jiao, Shuliang
author_sort Zhang, Xiangyang
collection PubMed
description The ultimate goal of the study is to provide an imaging tool to detect the earliest signs of glaucoma before clinically visible damage occurs to the retinal nerve fiber layer (RNFL). Studies have shown that the optical reflectance of the damaged RNFL at short wavelength (<560nm) is reduced much more than that at long wavelength, which provides spectral contrast for imaging the earliest damage to the RNFL. To image the spectral contrast we built a dual-band spectral-domain optical coherence tomography (SD-OCT) centered at 808nm (NIR) and 415nm (VIS). The light at the two bands was provided by the fundamental and frequency-doubled outputs of a broadband Ti:Sapphire laser. The depth resolution of the NIR and VIS OCT systems are 4.7µm and 12.2µm in the air, respectively. The system was applied to imaging the rat retina in vivo. Significantly different appearances between the OCT cross sectional images at the two bands were observed. The ratio of the light reflected from the RNFL over that reflected from the entire retina at the two bands were quantitatively compared. The experimental results showed that the dual-band OCT system is feasible for imaging the spectral contrasts of the RNFL.
format Online
Article
Text
id pubmed-3290519
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Optical Society of America
record_format MEDLINE/PubMed
spelling pubmed-32905192012-02-29 Dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer Zhang, Xiangyang Hu, Jianming Knighton, Robert W. Huang, Xiang-Run Puliafito, Carmen A. Jiao, Shuliang Opt Express Research-Article The ultimate goal of the study is to provide an imaging tool to detect the earliest signs of glaucoma before clinically visible damage occurs to the retinal nerve fiber layer (RNFL). Studies have shown that the optical reflectance of the damaged RNFL at short wavelength (<560nm) is reduced much more than that at long wavelength, which provides spectral contrast for imaging the earliest damage to the RNFL. To image the spectral contrast we built a dual-band spectral-domain optical coherence tomography (SD-OCT) centered at 808nm (NIR) and 415nm (VIS). The light at the two bands was provided by the fundamental and frequency-doubled outputs of a broadband Ti:Sapphire laser. The depth resolution of the NIR and VIS OCT systems are 4.7µm and 12.2µm in the air, respectively. The system was applied to imaging the rat retina in vivo. Significantly different appearances between the OCT cross sectional images at the two bands were observed. The ratio of the light reflected from the RNFL over that reflected from the entire retina at the two bands were quantitatively compared. The experimental results showed that the dual-band OCT system is feasible for imaging the spectral contrasts of the RNFL. Optical Society of America 2011-09-22 /pmc/articles/PMC3290519/ /pubmed/21996906 http://dx.doi.org/10.1364/OE.19.019653 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Research-Article
Zhang, Xiangyang
Hu, Jianming
Knighton, Robert W.
Huang, Xiang-Run
Puliafito, Carmen A.
Jiao, Shuliang
Dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer
title Dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer
title_full Dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer
title_fullStr Dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer
title_full_unstemmed Dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer
title_short Dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer
title_sort dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer
topic Research-Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290519/
https://www.ncbi.nlm.nih.gov/pubmed/21996906
http://dx.doi.org/10.1364/OE.19.019653
work_keys_str_mv AT zhangxiangyang dualbandspectraldomainopticalcoherencetomographyforinvivoimagingthespectralcontrastsoftheretinalnervefiberlayer
AT hujianming dualbandspectraldomainopticalcoherencetomographyforinvivoimagingthespectralcontrastsoftheretinalnervefiberlayer
AT knightonrobertw dualbandspectraldomainopticalcoherencetomographyforinvivoimagingthespectralcontrastsoftheretinalnervefiberlayer
AT huangxiangrun dualbandspectraldomainopticalcoherencetomographyforinvivoimagingthespectralcontrastsoftheretinalnervefiberlayer
AT puliafitocarmena dualbandspectraldomainopticalcoherencetomographyforinvivoimagingthespectralcontrastsoftheretinalnervefiberlayer
AT jiaoshuliang dualbandspectraldomainopticalcoherencetomographyforinvivoimagingthespectralcontrastsoftheretinalnervefiberlayer