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Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR
A major limitation in the clinical management and experimental research of leptospirosis is the poor performance of the available methods for the direct detection of leptospires. In this study, we compared real-time PCR (qPCR), targeting the lipL32 gene, with the immunofluorescent imprint method (IM...
Autores principales: | Chagas-Junior, Adenizar D., da Silva, Caroline L. R., Soares, Luciane Marieta, Santos, Cleiton S., M. Silva, Carlos D. C., Athanazio, Daniel A., dos Reis, Mitermayer G., Cruz McBride, Flávia W., McBride, Alan J. A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290571/ https://www.ncbi.nlm.nih.gov/pubmed/22393440 http://dx.doi.org/10.1371/journal.pone.0032712 |
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