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Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium

Maintaining proper cell–cell adhesion in the intestine is essential for tissue homeostasis and barrier function. This adhesion is thought to be mediated by cell adhesion structures, including tight junctions, adherens junctions, and desmosomes, which concentrate in the apical junctional region. Whil...

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Detalles Bibliográficos
Autores principales: Sumigray, Kaelyn D., Lechler, Terry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290639/
https://www.ncbi.nlm.nih.gov/pubmed/22238362
http://dx.doi.org/10.1091/mbc.E11-11-0923
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author Sumigray, Kaelyn D.
Lechler, Terry
author_facet Sumigray, Kaelyn D.
Lechler, Terry
author_sort Sumigray, Kaelyn D.
collection PubMed
description Maintaining proper cell–cell adhesion in the intestine is essential for tissue homeostasis and barrier function. This adhesion is thought to be mediated by cell adhesion structures, including tight junctions, adherens junctions, and desmosomes, which concentrate in the apical junctional region. While clear roles for adherens and tight junctions have been established in simple epithelia, the function of desmosomes has not been addressed. In stratified epithelia, desmosomes impart mechanical strength to tissues by organizing and anchoring the keratin filament network. In this paper, we report that the desmosomal protein desmoplakin (DP) is not essential for cell adhesion in the intestinal epithelium. Surprisingly, when DP is lacking, keratin filament localization is also unperturbed, although keratin filaments no longer anchor at desmosomes. Unexpectedly, DP is important for proper microvillus structure. Our study highlights the tissue-specific functions of desmosomes and reveals that the canonical functions for these structures are not conserved in simple epithelium.
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spelling pubmed-32906392012-05-16 Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium Sumigray, Kaelyn D. Lechler, Terry Mol Biol Cell Articles Maintaining proper cell–cell adhesion in the intestine is essential for tissue homeostasis and barrier function. This adhesion is thought to be mediated by cell adhesion structures, including tight junctions, adherens junctions, and desmosomes, which concentrate in the apical junctional region. While clear roles for adherens and tight junctions have been established in simple epithelia, the function of desmosomes has not been addressed. In stratified epithelia, desmosomes impart mechanical strength to tissues by organizing and anchoring the keratin filament network. In this paper, we report that the desmosomal protein desmoplakin (DP) is not essential for cell adhesion in the intestinal epithelium. Surprisingly, when DP is lacking, keratin filament localization is also unperturbed, although keratin filaments no longer anchor at desmosomes. Unexpectedly, DP is important for proper microvillus structure. Our study highlights the tissue-specific functions of desmosomes and reveals that the canonical functions for these structures are not conserved in simple epithelium. The American Society for Cell Biology 2012-03-01 /pmc/articles/PMC3290639/ /pubmed/22238362 http://dx.doi.org/10.1091/mbc.E11-11-0923 Text en © 2012 Sumigray and Lechler. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Sumigray, Kaelyn D.
Lechler, Terry
Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium
title Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium
title_full Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium
title_fullStr Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium
title_full_unstemmed Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium
title_short Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium
title_sort desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290639/
https://www.ncbi.nlm.nih.gov/pubmed/22238362
http://dx.doi.org/10.1091/mbc.E11-11-0923
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