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Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance

Assembly of an integral Golgi complex is driven by microtubule (MT)-dependent transport. Conversely, the Golgi itself functions as an unconventional MT-organizing center (MTOC). This raises the question of whether Golgi assembly requires centrosomal MTs or can be self-organized, relying on its own M...

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Autores principales: Vinogradova, Tatiana, Paul, Raja, Grimaldi, Ashley D., Loncarek, Jadranka, Miller, Paul M., Yampolsky, Dmitry, Magidson, Valentin, Khodjakov, Alexey, Mogilner, Alex, Kaverina, Irina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290642/
https://www.ncbi.nlm.nih.gov/pubmed/22262454
http://dx.doi.org/10.1091/mbc.E11-06-0550
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author Vinogradova, Tatiana
Paul, Raja
Grimaldi, Ashley D.
Loncarek, Jadranka
Miller, Paul M.
Yampolsky, Dmitry
Magidson, Valentin
Khodjakov, Alexey
Mogilner, Alex
Kaverina, Irina
author_facet Vinogradova, Tatiana
Paul, Raja
Grimaldi, Ashley D.
Loncarek, Jadranka
Miller, Paul M.
Yampolsky, Dmitry
Magidson, Valentin
Khodjakov, Alexey
Mogilner, Alex
Kaverina, Irina
author_sort Vinogradova, Tatiana
collection PubMed
description Assembly of an integral Golgi complex is driven by microtubule (MT)-dependent transport. Conversely, the Golgi itself functions as an unconventional MT-organizing center (MTOC). This raises the question of whether Golgi assembly requires centrosomal MTs or can be self-organized, relying on its own MTOC activity. The computational model presented here predicts that each MT population is capable of gathering Golgi stacks but not of establishing Golgi complex integrity or polarity. In contrast, the concerted effort of two MT populations would assemble an integral, polarized Golgi complex. Indeed, while laser ablation of the centrosome did not alter already-formed Golgi complexes, acentrosomal cells fail to reassemble an integral complex upon nocodazole washout. Moreover, polarity of post-Golgi trafficking was compromised under these conditions, leading to strong deficiency in polarized cell migration. Our data indicate that centrosomal MTs complement Golgi self-organization for proper Golgi assembly and motile-cell polarization.
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spelling pubmed-32906422012-05-16 Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance Vinogradova, Tatiana Paul, Raja Grimaldi, Ashley D. Loncarek, Jadranka Miller, Paul M. Yampolsky, Dmitry Magidson, Valentin Khodjakov, Alexey Mogilner, Alex Kaverina, Irina Mol Biol Cell Articles Assembly of an integral Golgi complex is driven by microtubule (MT)-dependent transport. Conversely, the Golgi itself functions as an unconventional MT-organizing center (MTOC). This raises the question of whether Golgi assembly requires centrosomal MTs or can be self-organized, relying on its own MTOC activity. The computational model presented here predicts that each MT population is capable of gathering Golgi stacks but not of establishing Golgi complex integrity or polarity. In contrast, the concerted effort of two MT populations would assemble an integral, polarized Golgi complex. Indeed, while laser ablation of the centrosome did not alter already-formed Golgi complexes, acentrosomal cells fail to reassemble an integral complex upon nocodazole washout. Moreover, polarity of post-Golgi trafficking was compromised under these conditions, leading to strong deficiency in polarized cell migration. Our data indicate that centrosomal MTs complement Golgi self-organization for proper Golgi assembly and motile-cell polarization. The American Society for Cell Biology 2012-03-01 /pmc/articles/PMC3290642/ /pubmed/22262454 http://dx.doi.org/10.1091/mbc.E11-06-0550 Text en © 2012 Vinogradova et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Vinogradova, Tatiana
Paul, Raja
Grimaldi, Ashley D.
Loncarek, Jadranka
Miller, Paul M.
Yampolsky, Dmitry
Magidson, Valentin
Khodjakov, Alexey
Mogilner, Alex
Kaverina, Irina
Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance
title Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance
title_full Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance
title_fullStr Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance
title_full_unstemmed Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance
title_short Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance
title_sort concerted effort of centrosomal and golgi-derived microtubules is required for proper golgi complex assembly but not for maintenance
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290642/
https://www.ncbi.nlm.nih.gov/pubmed/22262454
http://dx.doi.org/10.1091/mbc.E11-06-0550
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