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Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance
Assembly of an integral Golgi complex is driven by microtubule (MT)-dependent transport. Conversely, the Golgi itself functions as an unconventional MT-organizing center (MTOC). This raises the question of whether Golgi assembly requires centrosomal MTs or can be self-organized, relying on its own M...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290642/ https://www.ncbi.nlm.nih.gov/pubmed/22262454 http://dx.doi.org/10.1091/mbc.E11-06-0550 |
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author | Vinogradova, Tatiana Paul, Raja Grimaldi, Ashley D. Loncarek, Jadranka Miller, Paul M. Yampolsky, Dmitry Magidson, Valentin Khodjakov, Alexey Mogilner, Alex Kaverina, Irina |
author_facet | Vinogradova, Tatiana Paul, Raja Grimaldi, Ashley D. Loncarek, Jadranka Miller, Paul M. Yampolsky, Dmitry Magidson, Valentin Khodjakov, Alexey Mogilner, Alex Kaverina, Irina |
author_sort | Vinogradova, Tatiana |
collection | PubMed |
description | Assembly of an integral Golgi complex is driven by microtubule (MT)-dependent transport. Conversely, the Golgi itself functions as an unconventional MT-organizing center (MTOC). This raises the question of whether Golgi assembly requires centrosomal MTs or can be self-organized, relying on its own MTOC activity. The computational model presented here predicts that each MT population is capable of gathering Golgi stacks but not of establishing Golgi complex integrity or polarity. In contrast, the concerted effort of two MT populations would assemble an integral, polarized Golgi complex. Indeed, while laser ablation of the centrosome did not alter already-formed Golgi complexes, acentrosomal cells fail to reassemble an integral complex upon nocodazole washout. Moreover, polarity of post-Golgi trafficking was compromised under these conditions, leading to strong deficiency in polarized cell migration. Our data indicate that centrosomal MTs complement Golgi self-organization for proper Golgi assembly and motile-cell polarization. |
format | Online Article Text |
id | pubmed-3290642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-32906422012-05-16 Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance Vinogradova, Tatiana Paul, Raja Grimaldi, Ashley D. Loncarek, Jadranka Miller, Paul M. Yampolsky, Dmitry Magidson, Valentin Khodjakov, Alexey Mogilner, Alex Kaverina, Irina Mol Biol Cell Articles Assembly of an integral Golgi complex is driven by microtubule (MT)-dependent transport. Conversely, the Golgi itself functions as an unconventional MT-organizing center (MTOC). This raises the question of whether Golgi assembly requires centrosomal MTs or can be self-organized, relying on its own MTOC activity. The computational model presented here predicts that each MT population is capable of gathering Golgi stacks but not of establishing Golgi complex integrity or polarity. In contrast, the concerted effort of two MT populations would assemble an integral, polarized Golgi complex. Indeed, while laser ablation of the centrosome did not alter already-formed Golgi complexes, acentrosomal cells fail to reassemble an integral complex upon nocodazole washout. Moreover, polarity of post-Golgi trafficking was compromised under these conditions, leading to strong deficiency in polarized cell migration. Our data indicate that centrosomal MTs complement Golgi self-organization for proper Golgi assembly and motile-cell polarization. The American Society for Cell Biology 2012-03-01 /pmc/articles/PMC3290642/ /pubmed/22262454 http://dx.doi.org/10.1091/mbc.E11-06-0550 Text en © 2012 Vinogradova et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Vinogradova, Tatiana Paul, Raja Grimaldi, Ashley D. Loncarek, Jadranka Miller, Paul M. Yampolsky, Dmitry Magidson, Valentin Khodjakov, Alexey Mogilner, Alex Kaverina, Irina Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance |
title | Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance |
title_full | Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance |
title_fullStr | Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance |
title_full_unstemmed | Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance |
title_short | Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance |
title_sort | concerted effort of centrosomal and golgi-derived microtubules is required for proper golgi complex assembly but not for maintenance |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290642/ https://www.ncbi.nlm.nih.gov/pubmed/22262454 http://dx.doi.org/10.1091/mbc.E11-06-0550 |
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